Short communicationBreast cancer chemopreventive properties of pomegranate (Punica granatum) fruit extracts in a mouse mammary organ cultureMehta, R1; Lansky, E P2Author Information 1Department of Surgical Oncology, College of Medicine, University of Illinois at Chicago, Chicago, Illinois, USA 2Rimonest Ltd., Horev Center, Box 9945, Haifa, Israel Correspondence to: E P Lansky. Fax: (+972) 4 834 0966 E-mail: [email protected] Received 15 July 2003 Accepted 6 February 2004 European Journal of Cancer Prevention: August 2004 - Volume 13 - Issue 4 - p 345-348 doi: 10.1097/01.cej.0000136571.70998.5a Buy Metrics Abstract We previously reported anticancer effects of pomegranate extracts in human breast cancer cells in vitro and also chemopreventive activity of pomegranate fermented juice polyphenols (W) in a mouse mammary organ culture (MMOC). In the present study we decided to expand the MMOC investigations to also include an evaluation of the potential chemopreventive efficacy of a purified chromatographic peak of W (Peak B), and also of whole pomegranate seed oil. In brief, an MMOC was established according to a known method. For the first 10 days of culture, the glands were treated with pomegranate fermented juice polyphenols (W), a high-performance liquid chromatographic (HPLC) peak separated from W (peak B), or pomegranate seed oil (Oil, and on day 3, exposed to the carcinogen 7,12-dimethylbenz[a]anthracene (DMBA), and for 10 days treated with the putative pomegranate chemopreventive. The glands were subsequently harvested and tumours counted by visual inspection. While W effected a 42% reduction in the number of lesions compared with control, peak B and pomegranate seed oil each effected an 87% reduction. The results highlight enhanced breast cancer preventive potential both for the purified compound peak B and for pomegranate seed oil, both greater than that previously reported for pomegranate fermented juice polyphenols. © 2004 Lippincott Williams & Wilkins, Inc.