S01: Mapping the Air Pollution Metabolome: Applications, Limitations, and the Path Forward, Room 217, Floor 2, August 26, 2019, 10:30 AM - 12:00 PM
Background. Mechanisms underlying the effects of traffic-related air pollution on asthma etiology remain largely unknown, despite the abundance of observational and controlled studies reporting associations between traffic sources and asthma exacerbation and hospitalizations.
Objectives. We conducted the Atlanta Commuters Exposure (ACE-2) study: a crossover panel of commuters with and without asthma, to identify molecular pathways perturbed following traffic pollution exposures.
Methods. We measured 27 air pollutants and conducted high-resolution metabolomics profiling on blood samples from 45 commuters before and after each exposure session. We evaluated metabolite and metabolic pathway perturbations using an untargeted metabolome-wide association study framework with pathway analyses and chemical annotation.
Results. Most of the measured pollutants were elevated in highway commutes (p < 0.05). From both negative and positive ionization modes, 17,586 and 9,087 metabolic features were extracted from plasma, respectively. 494 and 220 unique features were associated with at least 3 of the 27 exposures, respectively (p<0.05), after controlling confounders and false discovery rates. Pathway analysis indicated alteration of several inflammatory and oxidative stress related metabolic pathways, including leukotriene, vitamin E, cytochrome P450, and tryptophan metabolism. We identified and annotated 45 unique metabolites enriched in these pathways, including arginine, histidine, and methionine. Most of these metabolites were not only associated with multiple pollutants, but also differentially expressed between participants with and without asthma. The analysis indicated that these metabolites collectively participated in an interrelated molecular network centering on arginine metabolism, underlying a potential etiology of traffic-related pollutants toxicity in individuals with asthma.
Conclusions. We detected numerous significant metabolic perturbations associated with in-vehicle exposures during commuting and validated metabolites that were closely linked to several inflammatory and redox pathways, elucidating the potential molecular mechanisms of traffic-related air pollution toxicity. These results support future studies of metabolic markers of traffic exposures, their corresponding molecular mechanisms, and asthma etiology.