Secondary Logo

Journal Logo

Institutional members access full text with Ovid®

Ultrastructural study of the possible protective effects of milk thistle seeds on the toxic effects of titanium dioxide nanoparticles in cardiac muscle of adult male albino rats

Mohammed, Doha S.; NorEldin, Eman K.

The Egyptian Journal of Histology: June 2016 - Volume 39 - Issue 2 - p 162–169
doi: 10.1097/01.EHX.0000482394.93661.d3
Original Articles

Background Titanium dioxide nanoparticles (TiO2NPs) are manufactured worldwide in large quantities for use in many applications, including manufacturing of pigments, plastics, paper, ink, food colorants, toothpastes, and cosmetics. Milk thistle is a herbal supplement used to treat toxic disorders, especially liver damage. Silymarin, an active ingredient of milk thistle, is a strong antioxidant that promotes liver cell regeneration and stabilizes cell membranes.

Aim The aim of this study was to investigate the histological changes in cardiac muscles after administration of different doses of TiO2NPs and the protective role of milk thistle in the possible toxic effects of TiO2NPs.

Materials and methods The present study was carried out on 15 adult male rats that were divided into five groups: group I, the control group; group IIa, which was injected with 100 mg/kg of TiO2; group IIb, which was treated with milk thistle orally during administration of 100 mg/kg of TiO2; group IIIa, which was injected with 150 mg/kg TiO2; and group IIIb, which was treated with milk thistle orally during administration of 150 mg/kg TiO2.

Results Changes in cardiomyocytes can be summarized as loss of their normal morphological appearance and appearance of degenerated myofibrils. Cardiomyocytes showed signs of apoptosis and degeneration as well as fragmented myofibrils with nuclear changes. These changes were improved by milk thistle in groups treated with low doses of TiO2.

Conclusion TiO2NPs are toxic to cardiomyocytes. Milk thistle may be of benefit when added to materials containing TiO2NPs and if used orally. More research is needed to verify the suitable dose and mode of administration of milk thistle.

Department of Histology, Faculty of Medicine, Sohag University, Sohag, Egypt

Correspondence to Doha S. Mohammed MD., Department of Histology, Faculty of Medicine, Sohag University, 82515, Sohag, Egypt Tel: +20 100 784 3762; fax: +20 934 602 963; e-mails:,

Received November 24, 2015

Accepted March 15, 2016

© 2016 The Egyptian Journal of Histology
You currently do not have access to this article

To access this article:

Note: If your society membership provides full-access, you may need to login on your society website