Secondary Logo

Journal Logo

Institutional members access full text with Ovid®

A histological and immunohistochemical study of different therapeutic modalities for experimentally induced ulcerative colitis in rats

El-Azab, Nahla El-Eraky; Salem, Mohamed Y.; Abd El-Salam, Sherifa

The Egyptian Journal of Histology: March 2016 - Volume 39 - Issue 1 - p 12–24
doi: 10.1097/01.EHX.0000481746.43677.e1
Original articles
Buy

Background Ulcerative colitis (UC) is a recurrent bowel disease. Several medications are used to treat patients with active UC. However, these are associated with side effects that add to the disease-related complications.

Aim The aim of the study was to evaluate the therapeutic effect of pentoxifylline, nanocurcumin, and mesenchymal stem cells on experimentally induced UC in rats.

Materials and methods Sixty-five adult male rats were divided into six groups: group I was the control group; group II (UC) consisted of rats that received a single intrarectal injection of 2 ml of 4% acetic acid; group ΙIΙ consisted of rats that received a daily oral dose of pentoxifylline starting 3 days after induction of colitis, for 2 weeks; group ΙV consisted of rats that received a daily oral dose of nanocurcumin starting 3 days after induction of colitis, for 2 weeks; group V comprised rats that received a single injection of bone marrow-derived mesenchymal stem cells through the tail vein 3 days after induction of colitis; and group VI consisted of rats that received a single intrarectal injection of 2 ml of 4% acetic acid and were left without treatment for 2 weeks. Colonic samples were processed and examined using histological and immunohistochemical techniques.

Results Group ΙIΙ showed improvement in the histopathological picture of colitis. Group IV showed a near-normal histological picture, except for a few areas of surface simple columnar cells that showed discontinuity and a few distorted crypts. Group V showed a histological picture that was nearly similar to that of the control group. Group VI showed a picture that was nearly similar to that of the UC group. There was a significant increase in alcian blue-positive reaction and a significant decrease in the amount of collagen fiber deposition and in tumor necrosis factor α expression (P<0.05) in groups III, IV, and V compared with group II, but these parameters were nonsignificant in the recovery group.

Conclusion Bone marrow-derived mesenchymal stem cells and nanocurcumin are more effective than pentoxifylline in the treatment against UC in rats. Nanocurcumin is cheaper and hence more cost-effective.

Department of Histology and Cell Biology, Faculty of Medicine, Benha University, Benha, Egypt

Correspondence to Nahla El-Eraky El-Azab, MD., Department of Histology and Cell Biology, Faculty of Medicine, Benha University, Benha, Egypt Tel.: +20 102 763 6357; fax: +013-3227518; e-mail: eleraky_nahla@yahoo.com

Received October 19, 2015

Accepted February 21, 2016

© 2016 The Egyptian Journal of Histology
You currently do not have access to this article

To access this article:

Note: If your society membership provides full-access, you may need to login on your society website