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Histological and immunohistochemical study of the effect of long period exposure to gold nanoparticles on the fundic mucosa of adult male albino rat

Soliman, Gehan M.a; El sherbeni, El Sayed M.b; Mostafa, Hossam El Din M.b

The Egyptian Journal of Histology: March 2015 - Volume 38 - Issue 1 - p 32–40
doi: 10.1097/01.EHX.0000459886.90155.9a
Original articles

Introduction Gold nanoparticles (GNPs) offer great promise in biomedical applications, such as intracellular gene regulation, chemotherapy, and drug delivery.

Aim of the work The aim of this study was to evaluate the immunohistochemical and histological effects of the oral administration of 10 nm GNPs on the fundic mucosa of albino rats.

Materials and methods A total of 30 adult male albino rats were used. They were divided into two groups. Group I (six animals) served as control. Group II, the experimental group (24 animals), received 100 μg/ml gold NPs daily by oral route. After 8 weeks, animals were sacrificed and the fundi of their stomachs were obtained for light and scanning electron microscopic studies.

Results Examination of H&E-stained sections of group II revealed deposition of GNPs in the glandular epithelium and the lamina propria of the mucosa. Focal areas of ulceration, vacuolation of glandular epithelium, and mononuclear cellular infiltration were seen with increased PAS-positive material and collagen fibers. Immunohistochemical study displayed significant increase in the number of nuclear Bcl-2-positive cells and CD68-positive cells. SEM examination revealed loss of normal architecture of the fundic mucosa.

Conclusion It could be concluded that long period exposure of adult albino rats to GNPs induced gold particle deposition in the fundic mucosa of the stomach, with histological and immunohistochemical changes.

aHistology Department, Faculty of Medicine, Tanta University, Tanta

bMedical Applications of Lasers (MAL) Department, National Institute of laser Sciences, Cairo University, Cairo, Egypt

Correspondence to Gehan M. Soliman, MD, Histology Department, Faculty of Medicine, Tanta University, 3111, Tanta, Egypt Tel: + 20403302545; fax: +20403334609; e-mail:

Received September 7, 2014

Accepted December 12, 2014

© 2015 The Egyptian Journal of Histology
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