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Effect of experimentally induced chronic pancreatitis on the structure of hepatocytes and the duodenal villi in adult male albino rats

Eltantaway, Eman H.; Khalaf, Gehan; Hamam, Ghada G.

The Egyptian Journal of Histology: March 2015 - Volume 38 - Issue 1 - p 1–9
doi: 10.1097/01.EHX.0000457300.50130.58
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Introduction Chronic pancreatitis (CP) is a serious disease affecting many organs but mainly the exocrine pancreas.

Aim The aim of the work was to evaluate the effect of experimentally induced CP on the structure of hepatocytes and the duodenal villi in adult male albino rats.

Materials and methods Twenty adult male albino rats were divided equally into two groups. Group I (the control group) received five intraperitoneal injections of saline at 3-day intervals. Group II (CP) was intraperitoneally injected five times at 3-day intervals with freshly prepared l-arginine solution at a dose of 500 mg/100 g body weight to induce CP. After 60 days of the first injection of l-arginine, rats were sacrificed and blood samples were collected to measure the level of random blood glucose. The pancreas, liver, and duodenum were processed for light microscopic study. The liver and duodenum were further processed for transmission electron microscopic and scanning electron microscopic study, respectively.

Results The light microscopic examination of the pancreas in group II (CP) revealed that most of the tissue had been replaced by fat cells. Some distorted pancreatic acini were seen. The islets of Langerhans and a few acini were comparable to that of the control group. The blood glucose level was nonsignificantly changed (P>0.05) compared with that of the control group. The liver of rats in group II (CP) showed vacuolated hepatocytes with multiple fat droplets. The duodenal villi of rats in group II (CP) showed indistinct boundaries between the enterocytes with an irregularity of their nuclei. Some of the nuclei appeared rounded, faint, and central in position. The number of intraepithelial lymphocytes was significantly higher than that of the control group.

Conclusion CP produced fat accumulation in hepatocytes and structural changes in the duodenal villi.

Histology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt

Correspondence to Gehan Khalaf, PhD, Histology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt Tel: +20 106 057 0468; fax: +20224445137; e-mail: dr_gehankhalaf@hotmail.com

Received October 29, 2014

Accepted November 7, 2014

© 2015 The Egyptian Journal of Histology
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