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Middle Ear Muscle Reflex and Word Recognition in “Normal-Hearing” Adults

Evidence for Cochlear Synaptopathy?

Mepani, Anita M.1,5; Kirk, Sarah A.1,5; Hancock, Kenneth E.1,2; Bennett, Kara3; de Gruttola, Victor3; Liberman, M. Charles1,2,4; Maison, Stéphane F.1,2,4

Author Information
doi: 10.1097/AUD.0000000000000804

Abstract

INTRODUCTION

Acoustic overexposure can lead to hair cell damage, threshold elevation, degraded frequency tuning, and loss of important cochlear nonlinearities (e.g., Liberman & Dodds 1984; Schmiedt 1984). A longstanding dogma was that hair cells are the primary targets of noise, and that cochlear neurons only die as a result of hair cell degeneration (Bohne & Harding 2000). Indeed, hair cell loss can be seen within hours of exposure, while loss of spiral ganglion neurons, the primary sensory nerves of the auditory pathway, is not detectable for months to years (Johnsson 1974; Johnsson & Hawkins 1976). According to this old view, cochlear neuropathy is a delayed downstream consequence of noise-induced hair cell loss, so that an exposure causing a temporary threshold elevation is benign and causes no permanent impairment. This assumption underlies the current damage-risk criteria for occupational noise exposure set by federal agencies (Arenas & Suter 2014).

Recent animal studies have altered the view that temporary threshold shift is always benign (Kujawa & Liberman 2009). After acoustic exposure, loss of synapses between cochlear neurons and surviving inner hair cells (IHCs) can occur, even if cochlear thresholds return to normal. Loss of synapses between primary afferents and sensory cells is also seen in the aging ear (Sergeyenko et al. 2013) or after exposure to ototoxic drugs (Bourien et al. 2014; Kujawa & Liberman 2015; Ruan et al. 2014). This cochlear synaptopathy remained undetected because the synapse is not visible in routine histologic preparations, and the ultimate loss of spiral ganglion cells is extremely slow (Liberman & Kiang 1978). Cochlear synaptopathy is also “hidden” because neural degeneration per se does not elevate behavioral or electrophysiologic thresholds until it becomes extreme (Lobarinas et al. 2013; Woellner & Schuknecht 1955). This is true, in part, because the most vulnerable cochlear neurons, to both noise and aging, are those with high thresholds and low (and medium) spontaneous rates (SRs) (Furman et al. 2013; Schmiedt et al. 1996). These low-SR fibers are major players in the coding of transient stimuli in noisy environments (Costalupes et al. 1984) because the high-SR fibers saturate in noise by virtue of their low thresholds and limited dynamic range. Together, these findings suggest that cochlear synaptopathy could be a contributor to poorer speech discrimination observed in older or noise-exposed patients (Alvord 1983; Dubno et al. 1984; Kujawa & Liberman 2015; Rajan & Cainer 2008). It also may be important in limiting psychophysical performance among human listeners with normal-hearing sensitivity, as deficits in binaural temporal processing are highly correlated with changes in auditory brain stem response (ABR) responses consistent with cochlear synaptopathy (Bharadwaj et al. 2014, 2015). Cochlear synaptopathy may also be key to the genesis of hyperacusis and tinnitus (Hickox & Liberman 2014; Knipper et al. 2013), via induction of central gain changes secondary to loss of afferent input to the auditory central nervous system (Hesse et al. 2016).

In animal studies of noise and aging, cochlear synaptopathy has been diagnosed by suprathreshold amplitude of ABR wave I (Kujawa & Liberman 2009, 2015; Shaheen et al. 2015), the summed activity of cochlear neurons. The fractional reduction in responses to moderate-level (60 to 80 dB SPL) tone-pips is correlated with the fractional reduction in synaptic counts in appropriate cochlear regions (Sergeyenko et al. 2013). In humans, the diagnostic utility of ABR wave I for cochlear synaptopathy remains controversial (Grinn et al. 2017; Tufts & Skoe 2018; Bramhall et al. 2019), possibly because, for ABR wave I amplitude in humans, intersubject variability is considerable, due to heterogeneity in head shape/size, tissue conductivity, sex, etc (Nikiforidis et al. 1993). However, recent histopathologic studies clearly show that cochlear synaptopathy is widespread in human ears, at least in age-related hearing loss (Wu et al. 2019).

Recent data from animal studies suggest that the middle ear muscle reflex (MEMR) may be a sensitive metric of cochlear synaptopathy in animals with normal thresholds (Valero et al. 2016, 2018), as suggested by the longstanding speculation that low-SR cochlear nerve fibers may be especially important in driving this sound-evoked feedback (Kobler et al. 1992; Liberman & Dodds 1984). The test was first introduced for the diagnosis of middle ear pathology. Years later, it was shown that, in the absence of conductive hearing loss, the MEMR could also be useful to assess (1) “retrocochlear pathology” such as vestibular schwannoma (Stach 1987) or auditory neuropathy (Berlin et al. 2005) and (2) “third-window” lesions of the inner ear such as semicircular canal dehiscence and enlarged vestibular aqueduct (Merchant & Rosowski 2008). Historically, the diagnostic power of the MEMR has seemed limited, given the wide range of test results in “normal-hearing” people (Margolis 1993; McGregor et al. 2018). However, some of this “normal” variation could be due to underlying cochlear synaptopathy, as suggested by the correlation, among normal-hearing subjects, between noise-induced tinnitus and MEMR strength (Wojtczak et al. 2017). On the other hand, other recent studies failed to see a relationship between classic measures of the acoustic reflex and tinnitus or speech-in-noise performance (Guest et al. 2018).

The present study aims at assessing the utility of the MEMR in the assessment of cochlear synaptopathy in humans by comparing MEMR measures with electrocochleographic measures with respect to the correlations with word recognition scores. We recruited normal-hearing subjects with a wide range of performance on challenging word recognition tasks and found significant correlations between word scores and MEMR thresholds; however, the MEMR tests did not outperform the summating potential (SP)/action potential (AP) ratio extracted from auditory-evoked potentials in predicting word scores. The results are consistent with contributions of cochlear synaptopathy to the degradation of word recognition in challenging listening environments.

MATERIALS AND METHODS

Subject Pool, Cognitive Assessment, and Inclusion Criteria

A total of 165 subjects in good health, between 18 and 63 years of age, with no history of ear or hearing problems, no history of neurologic disorders, and unremarkable otoscopic examinations were recruited as subjects. All had normal audiometric thresholds from 0.25 to 8 kHz in both ears (≤25 dB HL) with no interaural asymmetry and normal middle ear function. Thresholds were considered asymmetrical if there was an interaural difference of ≥10 dB at two test frequencies or ≥15 dB at one test frequency. Tympanometry was performed using the Titan Suite from Interacoustics, with a probe-tone frequency of 226 Hz and an ear-canal pressure change ranging from −300 to +200 daPa in each ear to ensure that ear-canal volume, tympanic membrane mobility, and middle ear pressure were within normal limits, as defined by Margolis and Heller (1987). There were no other initial inclusion criteria beyond the ability to give voluntary informed written consent before participation. This study was reviewed and approved by the Institutional Review Board of the Massachusetts Eye & Ear.

The Montreal Cognitive Assessment (MoCA) was administered to all subjects to screen for mild cognitive dysfunction related to deficits in attention and concentration, executive functions, memory, language, visuoconstructional skills, conceptual thinking, calculations, and orientation. The test was administered following the MoCA Version 8.1 Administration and Scoring Instructions (www.mocatest.org).

Audiometric Thresholds and Distortion Product Otoacoustic Emissions

Audiometric thresholds were obtained using Interacoustics Equinox 4.0 with the high hertz option. Pure-tone air conduction (AC) thresholds were measured at standard audiometric frequencies from 0.25 to 8 kHz and also at 3 and 6 kHz using DD45 headphones. To minimize changes in sound levels due to standing waves and improve intrasubject reliability of threshold estimates above 8 kHz, we measured AC thresholds at extended high frequencies (EHFs: 9, 10, 11.2, 12.5, 14, and 16 kHz) using warble tones delivered via a circumaural HDA200 high-frequency headset.

Distortion product otoacoustic emissions (DPOAEs) provide an objective, rapid, and independent measure of cochlear amplifier function. To complement behavioral audiometry, we measured DPOAEs as amplitude versus level functions with two primary tones f1 and f2 (f2/f1 = 1.22) with f2 = 0.5, 1, 2, 4, or 6 kHz in either ear (random selection), using the Interacoustics Titan v.3.4.0. For DPOAEs generated at f2 = 8, 11.2, 12.5, 14, and 16 kHz, stimulus generation and data acquisition were handled by a custom rig based on 24-bit digital input–output boards from National Instruments in a PXI chassis, with custom software control via LabVIEW. Response and stimulus waveforms, to and from the input–output boards, were transduced via microphone and dual-sound sources in an ER-10X system (Etymotics Research). DPOAEs were measured in both ears as amplitude versus level functions in 5 dB steps from 5 dB below threshold to 80 dB SPL. The DPOAE at 2f1–f2 was extracted from the ear-canal sound pressure after both time-domain and spectral averaging. Threshold was defined as the lowest level required to elicit a DPOAE >5 dB above the noise floor.

Middle Ear Muscle Reflex

Three assays of the MEMR were performed in both ears on each subject. Two are based on commercially available packages designed for clinical use, and a third was a custom assay based on animal experiments showing strong correlations between cochlear synaptopathy and MEMR strength (Valero et al. 2016). All these methods rely on the basic principle that middle ear muscle contractions evoked by an elicitor stimulus, presented either ipsilaterally or contralaterally, stiffen the ossicular chain and thereby change the ratio of absorbed and reflected sound measured in the ear canal in response to a probe stimulus.

The first clinical test, the acoustic reflex threshold (ART), used the Titan Suite from Interacoustics. It measures changes in acoustic admittance in response to an ipsilateral probe tone at 226 Hz evoked by an ipsilateral elicitor at 0.5, 1, 2, or 4 kHz, presented at increasing sound levels from 65 to 95 dB HL in 1 dB steps. Threshold at each elicitor frequency was defined as the lowest level producing a change in admittance >0.03 mmho. The final ART was defined as the averaged threshold obtained across the four elicitor frequencies.

Second, wideband tympanometry (WBT) was also performed using the Titan Suite from Interacoustics. The program measures changes in absorbance of an ipsilateral noise probe (≈65 dB nHL) evoked by a contralateral noise elicitor. Absorbance was measured (at atmospheric pressure) from 0.226 to 8 kHz with and without a white-noise elicitor at 95 dB SPL. Each condition was repeated twice in alternation. Absorbance spectra were averaged for each condition. Reflex strength was obtained by averaging spectral differences from 500 to 2000 Hz, where effects were the largest.

Last, MEMR effects were assessed using a custom method (MEMC) similar to that of Keefe et al. (2010), recently used to study cochlear synaptopathy in mice (Valero et al. 2016, 2018). Stimulus generation and data acquisition were controlled by the same custom rig used to measure high-frequency DPOAEs, using the ER-10X system as transducers to deliver and measure sound. As illustrated in Figure 1, this approach measures changes in ear-canal sound pressure to a click probe evoked by an ipsilateral noise elicitor. Specifically, we use a pair of 100-µsec clicks at 95 dB pSPL separated by a 500-msec elicitor (noise burst with a 2.5 msec ramp) presented 30 msec after the first click and preceding the second by 5 msec. This click-noise-click complex was repeated every 1535 msec, leaving 1 sec of silence between noise bursts to allow relaxation of the MEMs. Four complexes were presented at each elicitor level, and elicitor level was raised in 5 dB steps from 40 to 95 dB SPL. To eliminate click-evoked otoacoustic emissions, the waveforms were truncated at 2 msec after the peak of the click. For each ear, the whole process was repeated three times and averaged. For each average, the spectral difference (gain) between the two click waveforms was computed. Growth functions (gain versus elicitor level) were then displayed for each 500-Hz window from 500 to 5000 Hz. Threshold was assessed by visual inspection of these growth functions by two observers blinded to all other test results. Threshold was defined as the lowest elicitor level, for any of the frequency windows, at which the gain emerged from the noise floor. The mean interobserver difference was 0.71 dB ± 0.14 (standard error of the mean). To compute MEMR strength, the absolute values of the gain were summed across all frequencies from 500 to 5000 Hz, for an elicitor level of 95 dB SPL.

Fig. 1
Fig. 1:
Custom MEMR (MEMC) assay with a click-noise-click paradigm. A, Schematic of the stimulus complex for measuring MEMR threshold and strength. B–C, Data from one subject. Each curve in (B) is the spectrum of the difference in sound–pressure waveforms between the pre- and post-elicitor clicks at one elicitor level, color coded as shown. Gain vs. level functions (C) were derived by summing (at each elicitor level) the absolute spectral values (in dB) within a 500-Hz window, positioned where the signal to noise ratio was best. Threshold was defined by visual inspection of these growth functions from 2 observers blinded to the other test results. MEMR indicates middle ear muscle reflex.

Word Recognition

Of the many clinical speech-in-noise tests, we used the Northwestern university auditory test number six (NU-6) corpus (from Auditec, Inc.) and a modified version of the QuickSIN Speech-in-Noise test (from Etymotic Research, Inc.). We randomly selected one ear and presented four different NU-6 word lists of 50 consonant-vowel nucleus-consonant phonemically balanced words at 55 dB HL (≈75 dB SPL) under different conditions: (1) in the absence or presence of an ipsilateral speech-shaped noise masker (weighted random noise with a constant amplitude from 125 to 1000 Hz and falling 12 dB/octave from 1000 to 6000 Hz) at 0 dB signal to noise ratio (SNR) or (2) speeded up (“time compression”) at 45% or 65% with added reverberation (0.3 sec echo) (Noffsinger et al. 1994). Each participant was presented with the same lists in the same order. Our modified (m)QuickSIN test consisted of four lists of six sentences. Each sentence within one list was presented in the presence of a four-talker babble noise at decreasing SNRs (from 10 to 5, 3, 2, 1, and 0 dB SNR). Each sentence contained five key words. The first list of six sentences was used as practice. The overall score was obtained by averaging the number of correctly repeated key words (up to 30 per list) across the three subsequent lists.

Word scores from 26 participants were excluded because they were not native speakers of English (n = 12), were familiar with the word tests (n = 2), and/or failed the MoCA with a score <26 out of 30 (n = 12).

Electrocochleography

Stimuli were generated by our custom rig, stimulus waveforms were transduced via ER-3A insert earphones, and data acquisition was handled by the Interacoustics Eclipse computer system and software. Subjects' ear canals were prepped by scrubbing with a cotton swab coated in Nuprep. Electrode gel was applied on the cleaned portion of the canal and over the gold foil of ER3-26A/B tiptrodes before insertion. A horizontal montage was used, with a ground on the forehead at midline, one tiptrode as the inverting electrode and the other as the noninverting electrode in the opposite ear. Low (<5 kΩ) and balanced impedance readings were obtained with interelectrode impedance values within 2 kΩ of each other. Acoustic stimuli were delivered via silicone tubing connected to the ER-3A earphones. Stimuli were 100-μsec clicks delivered at 125 dB pSPL in alternating polarity at 9.1 Hz. Electrical responses were amplified 100,000× and 2000 sweeps were averaged, with artifact rejection enabled in the software. Average traces acquired by the Eclipse software (passband 3.3 to 5000 Hz) were exported for further analysis, including (optional) digital filtering with a 10 to 3000 Hz passband. The SP and AP peaks were defined by visual inspection by two observers blinded to all other test results. Interobserver reliability was assessed: discrepancies, observed in ≈10% of cases, were resolved while still blinded to the other test results. The SP peak was defined as the highest inflection point preceding the AP. The AP was defined in two ways: (1) as the difference between baseline and the maximum value from 1 to 2 msec post onset or (2) the difference between SP peak and the maximum value from 1 to 2 msec post onset. SP and AP identities were confirmed by comparing waveforms obtained at repetition rates of 9.1 versus 40.1 per second: a significant reduction of AP amplitude is seen at the higher presentation rate. The total noise dose for all electrocochleographic measurements was well within Occupational Safety and Health Administration and National Institute for Occupational Safety and Health standards.

Statistics

Four speech recognition measures were considered as outcome variables: the word recognition score in (1) noise, (2) with 45%, or (3) 65% time compression plus reverberation, and (4) the number of correct words on the modified QuickSIN (mQuickSIN) test. These outcome measures were not ear specific, so there is only one measure per subject. The following 14 measures were considered as predictors: (1) mean AC thresholds at standard frequencies, (2) mean AC thresholds at EHF, (3) mean DPOAE thresholds at standard frequencies, (4) mean DPOAE thresholds at EHFs, (5) MEMC threshold, (6) MEMc strength, (7) AR thresholds averaged across all four elicitor, (8) WBT strength summed from 500 to 200 Hz, (9) WBT strength summed from 256 to 8000 Hz, (10) SP amplitude, (11) AP amplitude defined as the difference between baseline and AP peak, (12) AP amplitude defined as the difference between SP peak and AP peak, (13) SP/AP ratio with AP defined as the difference between baseline and AP peak, and (14) SP/AP ratio with AP amplitude defined as the difference between SP peak and AP peak. All variables were measured in both ears except DPOAEs at standard frequencies. For all remaining predictors, we transformed the two measurements (one for each ear) into the mean of the two ears and the difference between the right ear and the mean, thereby including information from both ears in the models while avoiding collinearity.

Pearson correlation coefficients were used to assess the strength of the pairwise correlations between each predictor and each outcome measure. A Fisher r-to-Z transformation was used to assess the significance of the differences among correlation coefficients. To investigate combinations of variables in a multivariable regression, “stepwise selection methods” were then applied using all predictors to determine the best model separately for each outcome variable. The criterion for inclusion or exclusion from the model was a significance level of p = 0.10. Individual predictors were added to the model, in order of decreasing pairwise correlation, until the adjusted r2 stopped increasing. As a final step, the confounding variables of age (continuous) and sex (binary) were added to the models. Data were analyzed using SAS (SAS Institute, version 9.4).

RESULTS

Audiometric Thresholds

Threshold audiometry was performed on 165 subjects (93 females and 72 males), 18 to 63 years of age. All had normal thresholds (≤25 dB HL) from 0.25 to 8 kHz in both ears (Fig. 2A); however, significant threshold variability was observed at EHFs (9 to 16 kHz). Not surprisingly, mean EHF threshold was significantly correlated with age (Fig. 2B), with no obvious effect of sex. As shown in Figure 2A, 137 of the 165 subjects had mean EHF thresholds ≤20 dB HL, while the remaining 28 were worse. To further probe cochlear function, we measured DPOAEs. Mean DPOAE thresholds were highly correlated with mean AC thresholds for either standard audiometric frequencies (Fig. 2C) or EHFs (Fig. 2D).

Fig. 2
Fig. 2:
Threshold sensitivity was highly variable at extended high frequencies. A, AC thresholds for all subjects, color coded according to mean EHF thresholds (9 to 16 kHz), as shown in the key, along with the number of subjects falling within the mean EHF values cited. B, Mean AC thresholds at EHF were correlated with age. C–D, AC thresholds were correlated with corresponding DPOAE thresholds (f2 matched with audiometric frequency), both for standard audiometric frequencies (C) and EHFs (D). Correlation coefficients are shown for each panel. ***p < 0.001. AC indicates air conduction; DPOAE, distortion product otoacoustic emission; EHF, extended high frequency.

Word recognition performance was assessed using the NU-6 corpus because these lists of phonemically balanced words offer no contextual clues. When a list of 50 NU-6 words was presented monaurally at 55 dB HL in quiet, scores were excellent (≥96%) in all subjects (Fig. 3A). However, when word lists were presented in speech-shaped noise at 0 dB SNR or when words were time compressed (45% or 65%) with added reverberation, a large range in performance was observed (Figs. 3B–D). For instance, for words in noise, scores were as low as 18% and as high as 62% correct (Fig. 3B). A similar range of scores was observed with a modified version of the QuickSIN, a test based on phonemically balanced sentences in increasingly high-level background babble (Fig. 3E), with scores ranging from 10 to 26 correctly repeated key words out of 30.

Fig. 3
Fig. 3:
Distribution of word recognition scores. Histograms show the score distribution for each word recognition test (A–D) or for the mQuickSIN test (E). mQuickSIN indicates modified QuickSIN.

Given the wide range of EHF thresholds, as measured by either AC or DPOAEs (Fig 2A), it was important to examine the effect of thresholds on word scores. As summarized in Figure 4A, there were statistically significant correlations (poorer thresholds—worse scores) between standard frequency averages (AC only, not DPOAEs) and scores on the 45% time-compressed words and the QuickSIN. There were stronger correlations between EHF averages (either AC or DPOAE) and both time-compression/reverberation tests and the QuickSIN test. However, none of these correlations was statistically significant after adjustment for age, as illustrated for the 65% time-compression test in Figures 4B, C. We also considered the relationship between word scores and three different measures of the pure-tone average (0.5–1–2 kHz, 1–2–4 kHz, and 1–2–3–4 kHz), as significant correlations have been observed among the speech recognition in noise test, words-in-noise, and NU-6 test in quiet (Wilson & Cates 2008). None of these relationships was statistically significant after adjustment for age.

Fig. 4
Fig. 4:
Thresholds were uncorrelated with word recognition scores, after adjustment for age and sex. A, Table shows correlation coefficients (r) and p values [before (p), and after (p A), adjusting for age and sex] obtained between word scores and thresholds, at standard or extended high frequencies, and as measured by AC or DPOAEs, as indicated. Shaded boxes indicate relations that were significant (p < 0.05) before adjusting for age and sex. None was significant after adjusting. B–C, Word scores vs. mean AC thresholds at EHFs for the 65% time-compression test, before (B) and after adjusting for age (C). Correlation coefficients are shown for each panel. AC indicates air conduction; DPOAE, distortion product otoacoustic emission; EHF, extended high frequency; mQuickSIN, modified QuickSIN.

Middle Ear Muscle Reflex

MEMR strength and/or threshold was assessed bilaterally in each subject using three methods. First, (1) we used a custom wideband method (MEMc) (Keefe et al. 2010) that uses a pair of click probes flanking an ipsilateral noise elicitor (Fig. 1A). Because the offset time constant of MEM effects is ≈100 msec (Pang & Guinan 1997), the ear-canal response to the second click is modified by lingering effects of MEM contraction on middle ear reflectance. In addition, we used two clinical tests: (2) the ART, where the acoustic admittance of a low-frequency probe is compared with and without an ipsilateral tonal elicitor; and (3) WBT, where changes in absorbance elicited by a contralateral white noise are measured with a broadband probe.

MEMc effects differed greatly across our normal-hearing subjects, with thresholds ranging from 45 to 95 dB SPL (Fig. 5A). Subjects with the lowest MEMc thresholds tended to have the highest reflex strength, and vice versa (Fig. 5B), and there was high degree of correlation between MEMc thresholds in the two ears (Fig. S1, Supplemental Digital Content 2, http://links.lww.com/EANDH/A577). The intersubject variability in MEMc thresholds was not associated with sex or with thresholds, either at standard frequencies or EHFs, as measured either behaviorally or by DPOAEs (Fig. S2, Supplemental Digital Content 3, http://links.lww.com/EANDH/A578). Intersubject variation in the ART was also uncorrelated with threshold at standard frequencies or EHFs, as measured behaviorally or by DPOAEs (data not shown).

Fig. 5
Fig. 5:
MEMc thresholds and strengths were highly variable. A, Box and whiskers plot of MEMC thresholds from each ear for all subjects, coded as shown in (B), defines a lower (<68 dB SPL), median, and upper quartile (>77 dB SPL) distribution of MEMC reflex thresholds. B, Box and whiskers plots of MEMC strength for the three MEMC threshold groups defined in (A). **p < 0.01, ***p < 0.001. MEMc indicates custom middle-ear-muscle reflex assay; Thr., threshold.

Comparing MEMc thresholds to word scores (Fig. 6) revealed significant negative correlations, even after adjusting for age and sex, for words in noise (Fig. 6A), words with 45% time compression (Fig. 6B), or words with 65% time compression (Fig. 6C). In contrast, the correlation with the mQuickSIN was not significant (Fig. 6D). Similarly, the ART was negatively correlated with performance on the all three word tasks after adjusting for age and sex, but not with the mQuickSIN (Fig. 6). Estimated correlations were not increased by separately considering any of the four elicitor frequencies that are combined into the ART measure (Supplementary Table 1, Supplemental Digital Content 1, http://links.lww.com/EANDH/A576).

Fig. 6
Fig. 6:
MEM reflex thresholds were correlated with word recognition scores. MEM reflex thresholds, as assessed with MEMc or ART for each subject, are plotted against % correct scores on the different word tests or the QuickSIN as indicated. Arrows indicate that no response was detected at any elicitor level. Regression lines are plotted only for statistically significant correlations obtained after adjusting the data shown in this figure for age and sex: *p < 0.05, ***p < 0.001. ART indicates acoustic reflex threshold; Comp, compression; MEM, middle ear muscle; MEMc, custom middle-ear-muscle reflex assay; mQuickSIN, modified QuickSIN.

It is interesting that the correlations between word scores and MEMR function were lower when measured as strength rather than threshold, either by the custom assay or by the WBT test (data not shown). Only for words with 45% time compression were the correlations with MEMR strength statistically significant after adjusting for age and sex (r = 0.27; p < 0.05).

Electrocochleography

To further probe the peripheral contributions to deficits in word recognition, we compared word scores with measures of cochlear function seen via electrocochleography. We measured click-evoked potentials from ear-canal electrodes and extracted the amplitudes of both the “SP” and the “AP,” as illustrated in Figure 7A. In animal studies (Sergeyenko et al. 2013; Shaheen et al. 2015), the suprathreshold AP is reduced by cochlear synaptopathy, while SP is not. These observations are consistent with the classic view that AP represents the summed activity of auditory nerve fibers (some of which are silenced by cochlear synaptopathy), whereas the SP is dominated by presynaptic potentials from hair cells (which remain intact) (Zheng et al. 1997; Durrant et al. 1998).

Fig. 7
Fig. 7:
Some electrocochleographic measures were highly correlated with word scores. A, The mean waveform (± SEM) for the click-evoked responses from all subjects is used to illustrate the two methods for measuring AP amplitude. B–D, SP amplitude (B) and the SP/AP ratio (D) were correlated with scores on the words presented with time compression (65%) and reverberation, whereas AP amplitude, when measured baseline to peak, was not (C). D, AP amplitudes were correlated with word scores, when AP was measured shoulder to peak, as illustrated in (A). Regression lines are shown for significant correlations obtained after adjusting the data shown in this figure for age and sex. **p < 0.01; ***p < 0.001. AP indicates action potential; SEM, standard error of the mean; SP, summating potential.

As noted with the SP/AP ratio in our previous study of word recognition in normal-hearing subjects (Liberman et al. 2016), there was a significant negative correlation between SP amplitude and word scores on all three tests, after adjusting for age and sex. Data for words with 65% time compression are shown in Figure 7B; for the other word recognition tests, the correlations were as follows: r = −0.34, p < 0.001 for noise at 0 dB SNR; r = −0.34, p < 0.001 for 45% time compression; and r = −0.28, p = 0.002 for mQuickSIN. As in our previous study, the correlation was even higher for the SP/AP ratio. Data for 65% time compression are shown in Figure 7; for the other outcome measures, the correlations were as follows: r = −0.35, p < 0.001 for noise at 0 dB SNR; r = −0.44, p < 0.001 for 45% time compression; and r = −0.39, p < 0.001 for mQuickSIN.

The strength, and statistical significance, of the correlations between word scores and AP amplitudes depended on how AP was measured. If measured from baseline to peak, there were no significant correlations (Fig. 7C). However, if measured from the SP shoulder to peak, the correlation with word scores was significant (Fig. 7E). Data for 65% time compression are shown in Figure 7. Correlation for the other outcomes was r = 0.14, p = 0.149 for noise at 0 dB SNR; r = 0.30, p = 0.006 for 45% time compression; and r = 0.32, p = 0.003 for mQuickSIN. Measuring from shoulder to peak is more appropriate here because (1) the use of ear-canal electrodes yields a relatively large SP and (2) the lower corner of our response filter (10 Hz) leaves the SP “pedestal” largely unattenuated within the 1 to 2 msec latency window of the AP. Given these considerations, expressing the AP amplitude re the SP shoulder should better approximate the measure of AP amplitude with minimal contamination from the SP.

Statistical Comparisons and Multivariable Models

A Pearson correlation coefficient matrix (Fig. 8) summarizes the strength and significance of the pairwise correlations between word scores and the functional assays used in this study. The lack of statistical significant correlation for DPOAEs and behavioral thresholds, after adjustment for age and sex, suggests that differences in cochlear amplifier function do not explain the differences in performance. The correlations between MEM reflex measures and word recognition performance suggest an antimasking role for the MEMs and/or a contribution of auditory nerve loss to the performance deficit. The further association between word recognition and both SP and AP amplitudes (in opposite directions) supports the existence of a peripheral deficit in participants with poor performance and are consistent with a cochlear neuropathy, although a presynaptic contribution cannot be excluded based on the overall pattern of results.

Fig. 8
Fig. 8:
Visual representation of the pairwise correlations between 12 assays and four word recognition scores. In this matrix of Pearson bivariate correlations, the diameter of each disk is proportional to unadjusted r values. Gray disks indicate lack of statistical significance after adjustment for age and sex. Blue and red disks indicate statistical significance after adjusting for age and sex (*p < 0.05, **p < 0.01, ***p < 0.001). The color indicates the slope of the regression (blue, r > 0; red, r < 0). AC indicates air conduction; AP, action potential; AR, acoustic reflex threshold; Ba-Pk, baseline to peak; Comp, compression; DPOAE, distortion product otoacoustic emission; EcochG, electrocochleography; EHF, extended high frequency; MEMc, custom middle-ear-muscle reflex assay; MEMR, middle ear muscle reflex; mQuickSIN, modified QuickSIN; Rev, reverberation; Sh-Pk, shoulder to peak; SP, summating potential; WBT, wideband tympanometry.

Multivariable regression (Table 1) showed that, once the SP/AP ratio (the single strongest predictor) is included in the model, addition of MEMR metrics provides minimal additional predictive power: in only one case (45% time compression with reverberation) do MEMR thresholds (ART) improve the prediction. Thus, MEMR and SP/AP effects are likely to share the same underlying mechanism. The latter suggests that poor performance in word recognition is likely to arise from the degradation of stimulus coding due to cochlear dysfunction that also attenuates the MEMR.

TABLE 1
TABLE 1:
Stepwise multivariate regression identifies the best model for each word test

DISCUSSION

Acoustic Injury, Cochlear Synaptopathy, and Hearing in Noise

Two people with the same hearing sensitivity can have very different speech discrimination scores, particularly in noisy environments (Vermiglio et al. 2012). The contribution of cochlear neural loss to this difference has always been a logical possibility; however, the present results, showing a significant correlation between SP/AP ratio and word scores in a large normal-hearing cohort, provide strong evidence for a peripheral contribution to these differences. Recent animal work has suggested that deafferentation of IHCs may be the rule rather than the exception in acquired sensorineural hearing loss, and that hair cell deafferentation occurs well before threshold elevation in the noise-exposed or aging ear (Kujawa & Liberman 2015; Liberman 2017). Recent human studies have corroborated the finding that the loss of auditory nerve connections to IHCs greatly outstrips the loss of IHCs themselves in the aging ear (Viana et al. 2015; Wu et al. 2019), and another human study suggests an association between poor word scores in quiet and the loss of auditory nerve peripheral axons in cases of presbyacusis with high-tone hearing loss (Felder & Schrott-Fischer 1995).

In animal studies of acoustic overexposure and aging, cochlear synaptopathy can be detected using the suprathreshold amplitude of ABR wave I (Kujawa & Liberman 2009, 2015; Shaheen et al. 2015). As long as cochlear thresholds remain normal, the fractional reduction in ABR responses is correlated with the fractional reduction in synaptic counts (Sergeyenko et al. 2013). Attempts to translate these animal results to human subjects with normal hearing sensitivity have produced mixed results (Bramhall et al. 2019). While some failed to observe an association between ABR wave I amplitude and noise exposure (Fulbright et al. 2017; Grinn et al. 2017; Guest et al. 2017, 2018; Prendergast et al. 2017; Spankovich et al. 2017), others have found signs of neural damage in aging populations (Johannesen et al. 2019; Grose et al. 2019) or cohorts likely to have suffered occupational or recreational overexposure (Bramhall et al. 2017, 2018; Grose et al. 2017; Liberman et al. 2016; Ridley et al. 2018; Skoe & Tufts 2018; Valderrama et al. 2018). Possible reasons for the lack of agreement include (1) difficulties in accurately estimating cumulative noise exposure, (2) significant intersubject differences in noise vulnerability, (3) lower noise vulnerability in humans compared with other mammals (Dobie et al. 2018), and (4) large variability in human ABR amplitudes, due to differences in head size, electrode impedance, etc (Nikiforidis et al. 1993).

In an attempt to reduce the intersubject variability in ABR amplitudes, we tried normalizing the neural peak (wave I or AP) to the SP peak (Liberman et al. 2016), thought to be dominated by contributions from the IHCs (Zheng et al. 1997; Durrant et al. 1998). This approach was inspired by animal work on aging and noise exposure, which showed that SP remained stable as wave I amplitude was reduced by synaptopathy (Sergeyenko et al. 2013), and by human electrocochleography showing that a robust SP remains, despite an attenuated or reduced AP, in people with genetic deafness arising from IHC synaptic dysfunction (Santarelli et al. 2009).

In a previous study, we noted that SP was enhanced, as AP was reduced, in those subjects assumed to have the worst acoustic overexposure (Liberman et al. 2016). These results echo a study of the acute effects of recreational music exposure traumatic enough to cause a 10-dB temporary threshold shift (Kim et al. 2005): click-evoked electrocochleography showed postexposure enhancement of SP coupled with an attenuation of AP. In our previous study, the high-risk group (with elevated SP/AP ratios) also performed more poorly on word recognition tests (the same battery as used here). Correspondingly, in the present study, elevated SP or SP/AP ratio was the predictors most robustly correlated with word scores (Fig. 8), and the SP/AP ratio dominated all the multivariate models derived for each of the word tests (Table 1).

These correlations from electrocochleography suggest a cochlear contribution to the differences in word scores among normal-hearing listeners. But how strong is the link to synaptopathy? The lack of correlation between word scores and thresholds, either behavioral or otoacoustic emission based, at either standard or EHF (Fig. 8), suggests that the differences cannot be ascribed to cochlear amplifier function, including the cochlear battery, as powered by the stria vascularis, which is required for normal DPOAEs (Mills 2006). By this logic, the only viable candidates are the IHC (mechanoelectric transduction or synaptic transmission) and/or the auditory nerve. Thus, changes in SP are not inconsistent with cochlear synaptopathy. However, at present, any explanation of SP enhancement would be highly speculative, for example, that it comprises a complex sum of presynaptic and postsynaptic potentials with different latencies and/or polarities (Pappa et al. 2019) such that reduction of a postsynaptic component (due to synaptopathy) could lead to an enhancement of the measured SP. A reduction in AP is pathognomonic for synaptopathy, at least when threshold sensitivity is still normal, but the present results suggest that an SP enhancement can mask an AP reduction, depending on the response-filtering protocols and response-measurement algorithms. SP and AP responses from humans with a genetic mutation compromising IHC synaptic transmission (Santarelli et al. 2009), and from animals in which the AP is acutely silenced by round-window application of a neural blocker (Yuan et al. 2014), both strongly suggest that the AP rides on top of the SP when the filter settings do not eliminate the steady state SP. Such an effect was likely pronounced in this study because SP is larger with an ear-canal electrode than with an earlobe electrode, for example. Differences in the conventions for measuring AP (or wave I), and the possible masking of an AP/wave-I reduction by a simultaneous SP enhancement, could contribute to negative results in other studies assessing the correlations between wave I amplitude and word recognition scores.

MEM Reflexes and Hearing in Noise

Here, we noted that MEM reflex thresholds were correlated with word scores in difficult listening environments, and data from noise-exposed animals have suggested that the MEM reflex is a sensitive measure of synaptopathy (Valero et al. 2016, 2018) that can be a better predictor of primary neural degeneration than wave I amplitude reduction. The underlying logic is that the high-threshold, low-SR auditory nerve fibers, which are the first to degenerate in the noise-exposure model (Furman et al. 2013) and the aging model (Lang et al. 2010), may also be important afferent drivers of the MEM feedback loop (Valero et al. 2016, 2018).

There are several clinical assays of MEM reflexes available in commercial audiology equipment. Because these assays are all relatively quick to administer, we chose to use two established tests (ART and WBT) and to design a third (MEMC). There are potentially important differences among the tests with respect to the probe stimuli (tones versus clicks versus noise), elicitor stimuli (ipsilateral versus contralateral and tones versus noise), and whether they measure only threshold or also suprathreshold reflex strength. Given data showing smaller MEM effects contralateral to the stimulated ear (Moller 1961), our custom assay uses an ipsilateral noise elicitor and an ipsilateral wideband probe to maximize sensitivity. Indeed, although the correlation between MEMR threshold assessment methods was significant (ART versus MEMC; r = 0.35; p < 0.001), the MEMc test produced the lowest reflex thresholds, regardless of which elicitor frequencies were included in the ART (Fig. 5).

The stapedius muscle, when activated, stiffens the ossicular chain and reduces sound transmission to the inner ear, especially for frequencies <1 kHz (Rabinowitz, Reference Note 1). A protective role has been suggested (Brask 1979), and people lacking the reflex are more vulnerable to acoustic injury in the workplace (Borg et al. 1983). However, the most important function of the reflex may be in preventing the upward spread of masking (Liberman & Guinan 1998; Pang & Guinan 1997).

Patients without acoustic reflex tend to show poorer speech recognition in quiet at high SPLs versus moderate SPLs (Borg & Zakrisson 1973; French & Steinberg 1947; Hannley & Jerger 1981; Jerger & Jerger 1971; McCandless & Goering 1974; Wormald et al. 1995), a phenomenon known as “rollover.” For example, patients with vestibular schwannoma, and absent MEM reflexes, have greater rollover compared with subjects with normal ART (Dorman et al. 1987; Hannley & Jerger 1981). With respect to noise masking, subjects without a measurable acoustic reflex performed more poorly on a sentence identification test compared with audiometrically matched control with a functional reflex (Anastasio & Momensohn-Santos 2005). Similarly, stapedectomy patients (who lack a functional MEMR) scored lower on speech tests in low-pass noise in the affected versus contralateral ear (Weisz et al. 2006), and these interaural differences were not seen when words were presented in quiet (Chadwell & Greenberg 1979).

MEM Reflexes and Cochlear Synaptopathy

Here, we show, in audiometrically normal adults, that word recognition scores in difficult listening situations are linked to MEMR thresholds (Fig. 6). This relationship could not be attributed to outer hair cell dysfunction because no correlation was detected between word scores and thresholds, either in the standard audiometric range or at EHF, as measured behaviorally and with DPOAEs (Fig. 8), after adjusting for age and sex.

Poor listening performance in subjects with high MEMR thresholds could arise either (1) directly from loss of the antimasking function of the MEMR, arising from dysfunction of the brainstem circuitry driving the reflex, or (2) from the degradation of stimulus coding due to cochlear dysfunction that also attenuates the reflex, or (3) from both mechanisms. In support of option (2), the electrocochleography results discussed earlier suggest that cochlear pathology may underlie both elevated MEMR thresholds and poor word scores. Further support derives from the observation that, once the SP/AP ratio is included in the multivariate predictor models, the addition of MEMR results only improved the predictive power in one of the four models (Table 1). The apparent redundancy of SP/AP and MEMR results in predicting word scores suggests a common mechanism, and the SP/AP changes suggest that the dysfunction is in the cochlea.

Additional hints that an attenuated MEM reflex may reflect underlying cochlear neural degeneration are provided by a recent study of normal-hearing subjects with and without tinnitus (Wojtczak et al. 2017). When suprathreshold growth of MEM reflex strength was assessed with a type of click-probe assay similar to that used here, a striking difference was observed. Subjects with tinnitus had significant weaker MEM reflexes than those without, consistent with emerging ideas that the loss of auditory nerve fibers is a key elicitor of the amplification in “central gain” that leads to the establishment and persistence of the tinnitus (Hesse et al. 2016).

The antimasking function of the MEMR arises because it acts as a high-pass filter with a cutoff at ≈1000 Hz (Rabinowitz, Reference Note 1). This filtering can minimize the upward spread of masking from low-frequency noise on high-frequency signals (Liberman & Guinan 1998) because, in the absence of the MEMR, low-frequency stimuli strongly suppress the responses of high-frequency auditory nerve fibers to stimuli near their best frequencies (Delgutte 1990). Indeed, the noise masker used here was low-pass filtered with a cutoff at 1000 Hz and a 12 dB/octave slope from 1000 to 6000 Hz. Thus, it is exactly the type of masker for which the MEMR should be particularly effective. On the other hand, the known antimasking effects of the MEMR cannot explain improved identification of time-compressed words with reverberation, and scores on both these tests also showed significant correlations with MEMR thresholds (Figs. 6B, C). Together, these observations also support the idea that cochlear pathology underlies the poor word scores, rather than the loss of the MEMR function per se.

The antimasking effect “would” be expected to improve performance on the mQuickSIN, which comprises sentences (Engineers 1969) from a female talker in four-talker babble. A robust MEMR should decrease the masking of (high-frequency) consonants by (low-frequency) speech babble with a spectrum dominated by vowels and peaking at ≈650 Hz (Killion et al. 2004). However, we did not observe a significant correlation between mQuickSIN scores and MEMR thresholds (Fig. 6D). This sentence-based test, with its increased importance of contextual clues, engages more high-level processing, such that differences in central auditory function may obscure the underlying differences in the quality of the signal coded in the response of the auditory nerve.

Another recent study failed to detect a correlation between MEMR thresholds and a speech in noise measure, among normal-hearing listeners (Guest et al. 2019). There are many possible reasons for a cross-study discrepancy such as this, including the fact that their speech-in-noise test was very different (binaural listening with spatially separated two-talker babble in a 16-alternative forced-choice test, scored as a signal to noise threshold criterion for 50% performance), their subject pool was smaller (n = 67), and their MEMR test was the ART. Here, we found that the ART did not correlate as well as did the custom assay with word scores (Fig. 6A versus Fig. 6E). Furthermore, using a bootstrapping approach, we randomly sampled 70-member subsets from our larger dataset and concluded that the correlations would have reached statistical significance in only one of the four speech tests (45% time compression with reverberation).

Future Directions

Establishing diagnostic indicators for cochlear synaptopathy in humans is important if we are to understand the prevalence of primary neural degeneration in clinical and “not-yet-clinical” human populations. Animal studies show that ear abuse at a young age exacerbates the progression of age-related hearing impairment (Fernandez et al. 2015). Thus, early diagnosis is critical in identifying those with “tender ears” who may already be incurring significant inner ear damage, long before there is elevation of standard audiometric thresholds. Furthermore, clarification of the true risks of noise exposure is important to public policy on noise abatement and to raising general consciousness about the dangers of ear abuse. On the basis of the present results, it seems unlikely that electrocochleography or a MEM reflex assay as presented here could diagnose the presence or absence of mild or moderate cochlear neural degeneration on a case-by-case basis. Although statistically significant, the correlation coefficients were weak and power to detect modest levels of correlation reflects the large number of participants. The interpretation of these tests is even more difficult if there is hair cell damage, and threshold elevation is superimposed on any neural damage. However, despite these difficulties, these tests may be useful in longitudinal studies to track the accumulation of neural degeneration in individual subjects. Recent animal research suggests that reconnecting surviving spiral ganglion cells to hair cells is possible after noise damage, by local delivery of growth factors to the round window (Sly et al. 2016; Suzuki et al. 2016). Thus, assays similar to those used here could conceivably be applied in a future clinical trial to track the repair of synaptic connections in human subjects.

ACKNOWLEDGMENTS

The authors gratefully acknowledge Mrs. Inge Knudson for coordinating subject recruitment. The authors thank Drs. J. J. Guinan, Jr., S. G. Kujawa, and M. D. Valero for their comments on earlier versions of this manuscript. The authors also gratefully acknowledge a gift from Decibel Therapeutics for the purchase of the commercial audiometric equipment. A.M.M. and S.A.K. performed the experiments and contributed equally to this work. K.E.H. developed software for data acquisition and analysis. K.B. and V.de.G. ran the statistical analyses. M.C.L. and S.F.M. designed the study and wrote the article. S.F.M. also performed experiments and data analysis.

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REFERENCE NOTE

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    Keywords:

    Acoustic reflex; Cochlear synaptopathy; Electrocochleography; Hearing in noise; Hidden hearing loss; Middle ear muscle reflex; Normal hearing; Word recognition

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