Original ArticleHigh-Performance Liquid Chromatography Using UV Detection for the Simultaneous Quantification of Ropivacaine and Bupivacaine in Human PlasmaGaudreault, François BSc*; Drolet, Pierre MD†; Varin, France BPharm, PhD*Author Information From the *Faculté de pharmacie, Université de Montréal; and †Département d'anesthésie-réanimation, Hôpital Maisonneuve-Rosemont, Montréal, Canada. Received for publication June 10, 2009; accepted August 15, 2009. Supported by Canadian Institutes of Health Reasearch (CIHR; MOP 84519). F. Gaudreault's studentship was supported by Faculté de pharmacie de l'Université de Montréal. Correspondence: France Varin, BPharm, PhD, Faculté de pharmacie, Université de Montréal, 2900 boulevard Edouard Montpetit, Succursale Centre-ville, Pavillon Jean Coutu, Québec H3T 1J4, Canada (e-mail: [email protected]). Therapeutic Drug Monitoring: December 2009 - Volume 31 - Issue 6 - p 753-757 doi: 10.1097/FTD.0b013e3181bc8014 Buy Metrics Abstract A specific high-performance liquid chromatography assay coupled with UV detection has been developed and validated for the simultaneous determination of ropivacaine and bupivacaine in human plasma. A liquid-liquid back extraction procedure was used to increase specificity, and very good and consistent recoveries were obtained: 93%-95% for ropivacaine and 90%-96% for bupivacaine. The lowest limit of quantification was 4 and 8 ng/mL for ropivacaine and bupivacaine, respectively. The method was sensitive, reproducible (coefficient of variation ≤ 6.9%), and accurate (96%-102%) and was applied successfully to study the pharmacokinetics of ropivacaine and bupivacaine in a perioperative context. © 2009 Lippincott Williams & Wilkins, Inc.