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A Simple and Fast Method for Quantification of Ertapenem using Meropenem as Internal Standard in Human Plasma in a Clinical Setting

D'Avolio, Antonio BSc, MSc; Baietto, Lorena BSc; De Rosa, Francesco Giuseppe MD; Garazzino, Silvia MD; Sciandra, Mauro; Siccardi, Marco BSc; Bonora, Stefano MD; Di Perri, Giovanni MD, DTM & H, PhD

doi: 10.1097/FTD.0b013e31815f4f7e
Original Article
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A high-performance liquid chromatography method for the determination of ertapenem in human plasma using ultraviolet detection (300 nm) was developed and validated. The method involved a simple organic protein precipitation that guarantees rapid sample preparation. By using meropenem as internal standard, it also guarantees improved precision (relative standard deviation <3.70) and accuracy (deviation from true value <6.58%). The method was linear from 0.59 to 150 μg/mL and reproducible. In conclusion, this method allows the measurement of ertapenem in human plasma and may be used for both clinical routine applications and pharmacokinetic studies.

From the Department of Infectious Diseases, University of Torino, Amedeo di Savoia Hospital, Corso Svizzera, Turin, Italy.

Received for publication April 2, 2007; accepted October 15, 2007.

Reprints: Antonio D'Avolio, BSc, MSc, Clinica delle Malattie Infettive, Ospedale Amedeo di Savoia Padiglione Q, Corso Svizzera 16410149, Torino, Italia (e-mail: antodav@inwind.it).

© 2008 Lippincott Williams & Wilkins, Inc.