ArticlesDetermination of Platinum Complexes in Clinical Samples by a Rapid Flameless Atomic Absorption Spectrometry AssayKloft, Charlotte* ; Appelius, Helge* ; Siegert, Wolfgang† ; Schunack, Walter*; Jaehde, Ulrich‡Author Information *Institute of Pharmacy, Department of Clinical Pharmacy, Free University of Berlin, Germany; †Charité — Virchow University Hospital, Department of Hematology and Oncology, Humboldt University of Berlin, Germany; and ‡Institute of Pharmacy, Department of Clinical Pharmacy, University of Bonn, Germany Received December 28, 1998; accepted July 18, 1999. Address correspondence and reprint requests to Ulrich Jaehde, PhD, Professor of Clinical Pharmacy, Institute of Pharmacy, University of Bonn, An der Immenburg 4, D-53121 Bonn, Germany. Therapeutic Drug Monitoring: December 1999 - Volume 21 - Issue 6 - p 631 Buy Abstract Summary The frequent use of platinum (Pt) complexes in cancer chemotherapy and the application of new therapeutic options and dosing strategies have increased the need for rapid analytic procedures to determine Pt concentrations in the biologic fluids of patients. Therefore a flameless atomic absorption spectrometry method for the quantification of Pt in plasma and ultrafiltrate was developed and validated. A simple sample preparation of only one dilution step was established. Only 400 μL of whole blood was required for duplicate analysis of Pt in both matrices. The matrix-specific temperature programs took less than 75 seconds. The lower limit of quantification was 40 ng Pt/mL and 20 ng Pt/mL for plasma and ultrafiltrate, respectively. Suitable linearity could be reached using separate calibration curves for the high and low Pt concentration ranges. Recovery of Pt was complete, and there were no major stability problems. The accuracy and precision of the new method met the international criteria for the validation of bioanalytic methods. In addition, the use of different anticoagulants for clinical sampling, ultrafiltration systems, and ultrafiltration conditions were investigated. The assay has already been extensively applied to pharmacokinetic studies. In conclusion, the new Pt assay proved to be rapid, simple, sensitive, and suitable for clinical use. © 1999 Lippincott Williams & Wilkins, Inc.