Original Articles: PDF OnlyMeasurement of Phenytoin in Serum Using In-House Reagents Employing the Syva Enzyme-Multiplied Immunoassay Technique PrincipleColbert, D. L. Author Information Department of Chemical Pathology, The Medical College of St. Bartholomew's Hospital, London, England Therapeutic Drug Monitoring: October 1992 - Volume 14 - Issue 5 - p 386-390 Buy Abstract Reagents have been developed for the measurement of phenytoin in serum, using the enzyme-multiplied immunoassay approach first introduced by Syva. The assay uses a commercially available antiserum and phenytoin valeric acid coupled to glucose-6-phosphate dehydrogenase as the tracer. It has been adapted for use on an Eppendorf EPOS analyser, which allows 300 assays/h with high precision. Excellent agreement was achieved with commercial reagents, and costs were reduced markedly. © Lippincott-Raven Publishers.
