Original Article: PDF OnlyPhenytoin and Phenobarbital Assayed by the ACCULEVEL Method Compared with EMIT in an Outpatient Clinic SettingMorris, Raymond G.; Schapel, Graham J.Author Information Department of Clinical Pharmacology, The Queen Elizabeth Hospital, Woodville, South Australia Therapeutic Drug Monitoring: December 1988 - Volume 10 - Issue 4 - p 469-473 Buy Abstract The ACCULEVEL (Syntex) therapeutic drug assay technique was evaluated for phenytoin and phenobarbital in 30 patients with epilepsy who attended a neurology outpatient clinic. This finger-prick whole blood method is calibrated by the manufacturer to give assay results equivalent to plasma drug concentration. The results were compared with EMIT (Syva) technique measurements on the plasma from venous blood drawn simultaneously. The results presented show regression lines of y = 0.91x + 10.8, (r2 = 0.92) and y = 0.97x + 5.01, (r2 = 0.77) for phenytoin and phenobarbital (mUM), respectively, when the ACCULEVEL finger-prick blood assay was compared with the EMIT venous plasma assay. Acceptable precision and accuracy data are presented for replicated ACCULEVEL assays. The ACCULEVEL method was found to be reliable when performed by a laboratory technician and provides a very convenient quantitative drug assay that could easily be performed by a variety of individuals at a site remote from laboratory facilities. © Lippincott-Raven Publishers.