Original Article: PDF OnlyAnalysis of Pemoline in Serum by High Performance Liquid Chromatography Clinical Application to Optimize Treatment of Hyperactive ChildrenTomkins, Christine P.; Soldin, Steven J.; MacLeod, Stuart M.; Rochefort, J. Guy; Swanson, James M.Author Information Departments of Clinical Biochemistry, Pediatrics and Pharmacology, University of Toronto, and Research Institute, Hospital for Sick Children, Toronto, Ontario, Canada Therapeutic Drug Monitoring: Volume 2 - Issue 3 - p 255-260 Buy Abstract Summary The analysis of pemoline (2-imino-5-phenyl-4-oxazolidine) by a rapid, sensitive, and specific high performance liquid chromatographic assay using ultraviolet detection is described. Only 100 μl of plasma or serum is required. Analytical recoveries of 88% for pemoline and 93% for the internal standard (4-methylprimidone) are obtained by this procedure. Between-day precision studies of serum controls containing 10.0, 2.0, and 0.5 mg pemoline/liter produce coefficients of variation of 6.2, 9.4, and 16.2%, respectively. A clinical study of 28 children treated with pemoline demonstrated a linear relationship between drug dose and serum concentrations with an apparent therapeutic range falling between 1.7 and 7.0 mg/liter. These serum concentrations were achieved at drug dosages between 37.5 and 112.5 mg/day. © Lippincott-Raven Publishers.