Propranolol in Serum by High Pressure Liquid Chromatography Using Nitrile (CN) Bonded Columns in Reverse Phase: PDF OnlyPropranolol in Serum by High Pressure Liquid Chromatography Using Nitrile (CN) Bonded Columns in Reverse PhaseSimon, M.; Terry, R.Author Information Bio-Science Laboratories, Van Nuys, California Address reprint requests to Dr. Simon at Research Department, Bio-Science Laboratories, 7600 Tyrone Avenue, Van Nuys, California 91405. Therapeutic Drug Monitoring: Volume 1 - Issue 2 - p 265-276 Buy Abstract Summary: A method for monitoring therapeutic levels of the betablocking and antiarrhythmic drug propranolol in serum has been developed as a high performance liquid chromatography technique using bonded nitrile columns in a reverse phase mode with an aqueous methanol mobile phase containing acetate as a counterion. The performance characteristics of eight different types of reverse phase columns are discussed with evaluation for use in the method. The present method combines basic organic (cyclohexane) extraction with fluorescence detection of the chromatographic effluent to provide a specific, rapid, precise, and reliable technique that has been successfully used to monitor propranolol therapy in several hundred patients without interference by propranolol metabolites or other drugs. The method is shown to compare well with other methods and is suitable for therapeutic drug monitoring by routine clinical laboratories. © Lippincott-Raven Publishers.