Secondary Logo

Journal Logo

Institutional members access full text with Ovid®

0.01% Hypochlorous Acid as an Alternative Skin Antiseptic

An In Vitro Comparison

Anagnostopoulos, Apostolos G. MD*; Rong, Andrew MD*; Miller, Darlene DHSc, MPH, CIC; Tran, Ann Q. MD*; Head, Trajen PhD; Lee, Michael C.§; Lee, Wendy W. MD*

doi: 10.1097/DSS.0000000000001594
Original Article
Buy

OBJECTIVE Compare the in vitro efficacy of hypochlorous acid 0.01% (HA), povidone iodine 5% (PI), chlorhexidine gluconate 4% (CHG), and isopropyl alcohol 70% (IPA) against common skin microorganisms.

MATERIALS AND METHODS Time-kill studies were conducted against methicillin-susceptible Staphylococcus aureus (MSSA) and Staphylococcus epidermidis (MSSE), methicillin-resistant S. aureus (MRSA) and S. epidermidis (MRSE), Candida albicans, Corynebacterium species (striatum and amycolatum), Propionibacterium acnes, Pseudomonas aeruginosa, Streptococcus pyogenes, Staphylococcus capitis, and Staphylococcus xylosus.

RESULTS Methicillin-resistant S. aureus: Bactericidal effect was immediate for HA and IPA. For PI and CHG, the effect occurred at 1 and 10 minutes, respectively. Methicillin-resistant S. epidermidis: Hypochlorous acid, IPA, and PI had immediate bactericidal effects, whereas CHG required 1 minute. Methicillin-susceptible Staphylococcus aureus: All agents had bactericidal effects at 1 minute. C. species, S. pyogenes, P. aeruginosa, and P. acnes: All antiseptics demonstrated immediate bactericidal effects. Methicillin-susceptible Staphylococcus epidermidis and S. capitis: Hypochlorous acid and IPA had immediate effect, whereas PI and CHG required 1 minute. C. albicans: Hypochlorous acid, IPA, and PI were immediately bactericidal, whereas CHG required 1 minute. S. xylosus: Hypochlorous acid and CHG were immediately bactericidal, whereas IPA and PI required 1 and 2 minutes, respectively.

CONCLUSION In vitro studies of HA 0.01% were observed to have equal or more efficacious antiseptic properties compared with IPA, CHG, and PI. Future studies will be needed to investigate its role in periocular use.

*Department of Ophthalmology, Oculoplastic & Reconstructive Surgery, Bascom Palmer Eye Institute, Miami, Florida;

Department of Microbiology, Bascom Palmer Eye Institute, Miami, Florida;

Department of Biochemistry and Molecular Biology, University of Miami, Miami, Florida;

§Westminster High School, Palmetto Bay, Florida

Address correspondence and reprint requests to: Wendy W. Lee, MD, Department of Ophthalmology, Oculoplastic and Reconstructive Surgery, Bascom Palmer Eye Institute, 900 NW 17th Street, Miami, FL 33136, or e-mail: Wlee@med.miami.edu

Avenova was supplied by Novabay Pharmaceuticals, Inc. for the purposes of this study.

The authors have indicated no significant interest with commercial supporters.

© 2018 by the American Society for Dermatologic Surgery, Inc. Published by Wolters Kluwer Health, Inc. All rights reserved.
You currently do not have access to this article

To access this article:

Note: If your society membership provides full-access, you may need to login on your society website