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Epidemiologic Analysis of Change in Eyelash Characteristics With Increasing Age in a Population of Healthy Women

Glaser, Dee A. MD*; Jones, Derek MD; Carruthers, Jean MD; Campo, Antoinette§; Moench, Susan PhD§; Tardie, Greg PhD§; Largent, Joan MPH, PhD; Caulkins, Carrie PhD

doi: 10.1097/DSS.0000000000000170
Original Article

BACKGROUND Observations that eyelashes become thinner, shorter, and lighter, as women age has not been previously quantified.

OBJECTIVE This study was conducted to investigate associations between eyelash characteristics and age.

MATERIALS AND METHODS The upper natural eyelashes of 179 subjects were photographed and analyzed (digital image analysis); length, thickness, and darkness (intensity: 0 = white and 255 = black) were calculated. Linear regression, including race as a potentially confounding factor, was used to assess the association between age and mean eyelash characteristics.

RESULTS Subjects' mean age was 40.3 (±10.3) years; 46.1% were white, 36.5% Asian, 9.0% Hispanic, 5.1% East Indian, and 3.4% black. Mean eyelash length ranged from 6.39 (±1.02) to 7.98 (±1.15) mm (subjects aged 50–65 years and 22–29 years, respectively). Mean thickness ranged from 1.17 (±0.42) to 1.62 (±0.56) mm2 (subjects aged 50–65 years and 20–29 years, respectively). Mean intensity ranged from 118.2 (±19.8) to 129.4 (±17.3) (subjects aged 30–39 years and 50–65 years, respectively). Adjusted for race, eyelash length, thickness, and darkness decreased significantly with increasing age (p < .000, p = .0090, and p < .05, respectively).

CONCLUSION Advancing age among an ethnically diverse population of healthy women is associated with significant decreases in eyelash length, thickness, and darkness.

*Department of Dermatology, Saint Louis University, St. Louis, Missouri;

Division of Dermatology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California;

Department of Ophthalmology and Visual Sciences, University of British Columbia, Vancouver, British Columbia;

§Scientific Communications and Information, Parsippany, New Jersey;

Allergan, Inc., Irvine, California

Address correspondence and reprint requests to: Dee A. Glaser, MD, Department of Dermatology, Saint Louis University, 4th Floor, 1755 S Grand Boulevard, St. Louis, MO 63104, or e-mail:

Supported by Allergan, Inc. Writing and editorial assistance provided by Scientific Communications and Information.

D. A. Glaser, D. Jones, and J. Carruthers are consultants and investigators for Allergan, Inc. J. Largent and C. Caulkins are employees and stockholders of Allergan, Inc. At the time of manuscript preparation, A. Campo, S. Moench, and G. Tardie were employees of Scientific Communications and Information, which received compensation from Allergan, Inc. for medical writing and editorial services.

© 2014 by the American Society for Dermatologic Surgery, Inc. Published by Lippincott Williams & Wilkins
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