To evaluate the growth factor responses associated with myocardial angiogenesis
Mice were treated with transmyocardial revascularization (TMR) and evaluated for angiogenic and growth factor responses.
TMR was performed via thoractomy with a 27 g needle. At 2, 5, and 7 days post-treatment, hearts were removed from the TMR treated and control groups, then assayed for angiogenesis
, fibroblast growth factor
(FGF)-2 expression and vascular endothelial cell growth factor
TMR caused an angiogenic reaction in the myocardial blood vessels at 7 days post-TMR treatment. Elevated FGF-2 corresponded with increased TMR related angiogenesis
. VEGF levels only increased in hearts that were prewounded then TMR treated.
The data show that TMR stimulates myocardial angiogenesis
. The angiogenic reaction is mediated by FGF-2 which increased in most experimental treatment groups. The VEGF response was more specific, requiring prewounding then TMR treatment for a VEGF increase.