Basic InvestigationIn Situ Ultrahigh-resolution Optical Coherence Tomography Characterization of Eye Bank Corneal Tissue Processed for Lamellar KeratoplastyBrown, Jamin S MD*; Wang, Danling PhD†; Li, Xiaoli BS†; Baluyot, Florence MS†; Iliakis, Bernie MHA‡; Lindquist, Thomas D MD, PhD‡; Shirakawa, Rika MD*§; Shen, Tueng T MD, PhD*†; Li, Xingde PhD†Author Information From the *Department of Ophthalmology and †Department of Bioengineering, University of Washington, Seattle, WA; the ‡Northwest Lions Foundation for Sight and Hearing, Seattle, WA; and the §Department of Ophthalmology, University of Tokyo, Tokyo, Japan. Received for publication September 28, 2007; revision received December 18, 2007; accepted January 19, 2008. Supported in part by the National Science Foundation Career Award (X.L.) and Eye Bank Association of America Scientific Research Award and Northwest Lions research grant (T.S.). Reprints: Tueng T. Shen, Department of Ophthalmology, University of Washington, Box 356485, Seattle, WA 98195 (e-mail: firstname.lastname@example.org). Cornea: August 2008 - Volume 27 - Issue 7 - p 802-810 doi: 10.1097/ICO.0b013e318169d6b7 Buy Metrics Abstract Purpose: To use optical coherence tomography (OCT) as a noninvasive tool to perform in situ characterization of eye bank corneal tissue processed for lamellar keratoplasty. Methods: A custom-built ultrahigh-resolution OCT (UHR-OCT) was used to characterize donor corneal tissue that had been processed for lamellar keratoplasty. Twenty-seven donor corneas were analyzed. Four donor corneas were used as controls, whereas the rest were processed into donor corneal buttons for lamellar transplantation by using hand dissection, a microkeratome, or a femtosecond laser. UHR-OCT was also used to noninvasively characterize and monitor the viable corneal tissue immersed in storage medium over 3 weeks. Results: The UHR-OCT captured high-resolution images of the donor corneal tissue in situ. This noninvasive technique showed the changes in donor corneal tissue morphology with time while in storage medium. The characteristics of the lamellar corneal tissue with each processing modality were clearly visible by UHR-OCT. The in situ characterization of the femtosecond laser-cut corneal tissue was noted to have more interface debris than shown by routine histology. The effects of the femtosecond laser microcavitation bubbles on the corneal tissue were well visualized at the edges of the lamellar flap while in storage medium. Conclusions: The results of our feasibility study show that UHR-OCT can provide superb, in situ microstructural characterization of eye bank corneal tissue noninvasively. The UHR-OCT interface findings and corneal endothelial disc thickness uniformity analysis are valuable information that may be used to optimize the modalities and parameters for lamellar tissue processing. The UHR-OCT is a powerful approach that will allow us to further evaluate the tissue response to different processing techniques for posterior lamellar keratoplasty. It may also provide information that can be used to correlate with postoperative clinical outcomes. UHR-OCT has the potential to become a routine part of tissue analysis for any eye bank or centers creating customized lamellar corneal tissue for transplantation. © 2008 Lippincott Williams & Wilkins, Inc.