Basic InvestigationUbiquinol Supplementation of Donor Tissue Enhances Corneal Endothelial Cell Mitochondrial RespirationSkeie, Jessica M. PhD*,†; Aldrich, Benjamin T. PhD*,†; Nishimura, Darryl Y. PhD*,†; Schmidt, Gregory A. MBA, CEBT†; Zimmerman, M. Bridget PhD‡; Ling, Jennifer J. MD*,†; Naguib, Youssef W. PhD§; Salem, Aliasger K. PhD§; Greiner, Mark A. MD*,† Author Information *Department of Ophthalmology and Visual Sciences, Carver College of Medicine, University of Iowa, Iowa City, IA; †Iowa Lions Eye Bank, Coralville, IA; ‡Department of Biostatistics, College of Public Health, University of Iowa, Iowa City, IA; and §Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy, University of Iowa, Iowa City, IA. Correspondence: Mark A. Greiner, MD, Cornea and External Diseases, Department of Ophthalmology and Visual Sciences, Carver College of Medicine, University of Iowa, 200 Hawkins Drive, Iowa City, IA 52242 (e-mail: [email protected]). Eversight Eye and Vision Research Grant, awarded July 1, 2016. The authors have no funding or conflicts of interest to disclose. Cornea: October 2020 - Volume 39 - Issue 10 - p 1285-1290 doi: 10.1097/ICO.0000000000002408 Buy Metrics Abstract Purpose: To determine whether ubiquinol improves mitochondrial function and cell viability in human donor corneal endothelial cells during hypothermic corneal tissue storage. Methods: Endothelial cell Descemet membrane tissues were treated with 10 μM ubiquinol, the reduced form of the antioxidant coenzyme Q10, for 5 days in Optisol-GS storage media before assaying for mitochondrial activity using extracellular flux analysis of oxygen consumption. In addition, endothelial cell Descemet membrane tissues were analyzed for cell viability using apoptosis and necrosis assays. Control tissues from mate corneas were treated with diluent only, and comparisons were analyzed for differences. Results: A total of 13 donor corneal tissues with a mean (SEM) preservation time of 11.8 days (0.4) were included for the analysis. Treatment with 10 μM ubiquinol increased spare respiratory capacity by 174% (P = 0.001), maximal respiration by 93% (P = 0.003), and proton leak by 80% (P = 0.047) compared with controls. Cells treated with ubiquinol had no significant change in cell necrosis or apoptosis. Conclusions: Preliminary testing in donor corneal tissue at specified doses indicates that ubiquinol may be a useful biocompatible additive to hypothermic corneal storage media that increases corneal endothelial cell mitochondrial function. Additional investigations are indicated to further study and optimize the dose and formulation of ubiquinol for use in preserving donor corneal tissue function during hypothermic storage. Copyright © 2020 Wolters Kluwer Health, Inc. All rights reserved.