First Report on Detection of Dengue Virus in the Donor CorneaJanani, Madhuravasal Krishnan, PhD; Durgadevi, Parthasarathy, PhD; Padmapriya, Jayavel, MSc; Malathi, Jambulingam, PhD; Kulandai, Lily Therese, PhD; Rao Madhavan, Hajib Narahari, MD, PhD, FAMS, FIC PathCornea: December 2018 - Volume 37 - Issue 12 - p 1586–1589 doi: 10.1097/ICO.0000000000001706 Case Report Buy Abstract Author InformationAuthors Article MetricsMetrics Background: Dengue fever is the most prevalent form of flavivirus infection in humans. We have investigated whether corneoscleral tissue of the donor affected by dengue virus (DENV) harbors the virus. Purpose: To identify the risk for viral transmission through corneal transplants in areas where DENV circulates. Methods: Excised corneoscleral tissue from a cadaver with a history of viral hemorrhagic fever was analyzed using reverse transcriptase-polymerase chain reaction for the presence of DENV and chikungunya virus (CHIV). Results: DENV was detected in RNA extracted from the donor corneoscleral rim. Further genotyping of the viral isolate from the virus-infected cell harvest revealed DENV type 3 as the causative agent. CHIV was not detected. Conclusions: The data presented in this study recommend the implementation of polymerase chain reaction for detection of DENV and CHIV to analyze excised corneoscleral tissue of a donor with viral hemorrhagic fever. Larsen & Toubro Microbiology Research Centre, Vision Research Foundation, Chennai, India. Correspondence: Jambulingam Malathi, PhD, Larsen & Toubro Microbiology Research Centre, Vision Research Foundation, Sankara Nethralaya, No. 41, College Rd, Chennai 600 006, India (e-mail: email@example.com). The authors have no funding or conflicts of interest to disclose. Received March 22, 2018 Received in revised form June 04, 2018 Accepted June 12, 2018 Copyright © 2018 Wolters Kluwer Health, Inc. All rights reserved.