Ocular Surface Reconstruction with the Autologous Conjunctival Epithelium and Establishment of a Feeder-Free and Serum-Free Culture SystemYamagami, Satoru, MD, PhD*; Yokoo, Seiichi, PhD†; Sakimoto, Tohru, MD, PhD*Cornea: November 2018 - Volume 37 - Issue - p S39–S41 doi: 10.1097/ICO.0000000000001729 Article Buy Abstract Author InformationAuthors Article MetricsMetrics Abstract: Transplantation of the autologous cultured corneal limbal epithelium and oral mucosal epithelium is a standard technique for ocular surface reconstruction under corneal limbal stem cell deficiency. As an option for bilateral cases, we recommend utilization of autologous conjunctivae for ocular surface reconstruction. Autologous conjunctival epithelium sheet transplantation was effective for bilateral corneal limbal stem cell deficiency without symblepharon or severe keratinization. Moreover, we established a feeder-free and serum-free culture system of the limbal epithelium. This system can be applied for culturing conjunctival epithelia. Autologous cultured conjunctival epithelium transplantation is a practical option for treating bilateral corneal limbal stem cell deficiency. *Department of Ophthalmology, Nihon University School of Medicine, Tokyo, Japan; and †Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan. Correspondence: Satoru Yamagami, MD, PhD, Department of Ophthalmology, Nihon University School of Medicine, 30-1 Itabashi-ku, Tokyo 173-8610, Japan (e-mail: firstname.lastname@example.org). The authors have no funding or conflicts of interest to disclose. Received June 29, 2018 Accepted July 03, 2018 Copyright © 2018 Wolters Kluwer Health, Inc. All rights reserved.