The aim of this study was to describe the ultrastructure of the host–donor interface in the eye of a recently deceased patient, who had undergone Descemet membrane endothelial keratoplasty.
The eye was enucleated postmortem, and after standard decontamination, the corneoscleral button was excised, cut into 4 quadrants, and processed for light and transmission electron microscopy evaluation.
Transmission electron microscopy revealed close attachment of the donor's Descemet membrane to the host's stroma and projection of stromal collagen fibers into the interfacial matrix, resembling a normal “virgin” corneal architecture.
Ultrastructurally, an attached Descemet membrane endothelial keratoplasty graft closely resembles that of an unoperated, healthy eye with no appreciable adventitious or missing structures.
*Melles Cornea Clinic Rotterdam, Rotterdam, the Netherlands;
†Netherlands Institute for Innovative Ocular Surgery, Rotterdam, the Netherlands;
‡Amnitrans Eye Bank Rotterdam, Rotterdam, the Netherlands; and
§Department of Neuroscience, Erasmus Medical Center Rotterdam, the Netherlands.
Reprints: Gerrit R.J. Melles, MD, PhD, Netherlands Institute for Innovative Ocular Surgery, Laan op Zuid 88, 3071 AA, Rotterdam, the Netherlands (e-mail: email@example.com).
G. R. J. Melles is a consultant for DORC International/Dutch Ophthalmic, USA. The remaining authors have no funding or conflicts of interest to disclose.
Received January 23, 2014
Accepted April 13, 2014