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Subconjunctival Injection of Low-Molecular-Weight Heparin–Taurocholate 7 Inhibits Corneal Neovascularization

Yoon, Sam Young MD; Kim, Jae Yong MD, PhD; Kim, Eun-Soon PhD; Kim, Soo Yeon MS; Kim, Myoung Joon MD, PhD; Tchah, Hungwon MD, PhD

doi: 10.1097/ICO.0b013e3182a48009
Basic Investigation

Purpose: To evaluate the effects of a subconjunctival injection of low-molecular-weight heparin–taurocholate 7 (LHT7) on corneal neovascularization (CoNV) in rats.

Methods: Twenty-four Sprague–Dawley rats were divided into 4 groups of 6 animals each. Corneal centers were cauterized by the application of a silver/potassium nitrate solution for 8 seconds. Either 0.02 or 0.04 mL of 25 mg/mL of LHT7 (low- and high-LHT7 groups, respectively) was subconjunctivally injected on days 2 and 4 after the cauterization was done; 0.02 mL of 25 mg/mL of bevacizumab was injected into rats in the positive control group, with normal saline (NS) being administered to a negative control group. Digital photographs of the cornea were taken 1 and 2 weeks later to calculate the percentage of CoNV using the area of the neovascularized cornea. To compare the differences in CoNV between weeks 1 and 2, the change in CoNV was calculated by subtracting the percentage of CoNV at 1 week from that at 2 weeks.

Results: The percentage of CoNV did not differ among the 4 groups either 1 or 2 weeks after the cauterization (P > 0.05). In all groups except the NS group, the percentage of CoNV significantly decreased at 2 weeks compared with that at 1 week (all P < 0.05). Moreover, the changes of CoNV in the high-LHT7 and bevacizumab groups significantly decreased compared with that in the NS group (all P < 0.05). Two corneal stromal hemorrhages occurred, 1 in each LHT7 group.

Conclusions: Despite complications, including corneal stromal hemorrhage, subconjunctival injection of LHT7 attenuated CoNV after chemical cauterization.

*Department of Ophthalmology, Kangdong Sacred Heart Hospital, Hallym University College of Medicine, Seoul, Korea;

Department of Ophthalmology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea; and

Research Institute for Biomacromolecules, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea.

Reprints: Hungwon Tchah, Department of Ophthalmology, University of Ulsan College of Medicine, Asan Medical Center, 388-1 Pungnap-2dong, Songpa-gu, Seoul 138-736, Korea (e-mail:

S. Y. Yoon and J. Y. Kim contributed equally to this study.

The authors have no conflicts of interest to disclose.

Supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) and funded by the Ministry of Education, Science, and Technology (MEST; NRF-2010-0025662) and a grant (2012-464) from the Asan Institute for Life Sciences, Seoul, Korea.

Received March 27, 2013

Accepted July 10, 2013

Copyright © 2013 Wolters Kluwer Health, Inc. All rights reserved.