Clinical SciencePenetrating Keratoplasty With Small-Diameter and Glycerin-Cryopreserved Grafts for Eccentric Corneal PerforationsShi, Weiyun MD, PhD; Liu, Mingna MD; Gao, Hua MD, PhD; Li, Suxia MD; Wang, Ting MD; Xie, Lixin MDAuthor Information From the State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, Qingdao, China. Received for publication December 12, 2007; revision received September 12, 2008; accepted October 12, 2008. Supported by the Qingdao Municipal Science and Technology Bureau (02KGYSH-01). Reprints: Prof Lixin Xie, MD, State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, 5 Yanerdao Rd, Qingdao 266071, China (e-mail: [email protected]). Cornea: July 2009 - Volume 28 - Issue 6 - p 631-637 doi: 10.1097/ICO.0b013e318191b857 Buy Metrics Abstract Purpose: To report the outcome of penetrating keratoplasty with small-diameter and glycerin-cryopreserved grafts for eccentric corneal perforation. Methods: In this retrospective consecutive case series, 15 eyes of 15 patients with eccentric corneal perforations underwent small-diameter penetrating keratoplasties with glycerin-cryopreserved grafts. The corneal grafts used in this study were 2.5-4.5 mm in size and preserved at −20°C in pure glycerin for 6 months to 5 years. The donors were secured with 6-8 sutures that spared the visual axis. The follow-up ranged from 7 to 36 months. Clarity of the grafts was observed, and changes in visual acuity and keratometric astigmatism were analyzed. Results: Global integrity was achieved in all eyes. All grafts remained clear at the end of the follow-up time. No immune rejection was detected. No complicated cataract or secondary glaucoma was developed. The uncorrected visual acuity improved from preoperative 20/2000 ~ 20/60 to postoperative 20/40 ~ 20/20, and the best-corrected visual acuity after surgery was 20/30 or better. The mean value of keratometric astigmatism was 2.47 ± 0.78 diopters postoperatively. Conclusions: Small-diameter penetrating keratoplasty seems to be effective in treating various eccentric corneal perforations. Glycerin-cryopreserved donors may be a suitable alternative to fresh material for this technique, which solves the problem of shortage in supplying corneas and can be used in an emergency. Copyright © 2009 Wolters Kluwer Health, Inc. All rights reserved.