Basic InvestigationEfficacy and Safety of Voriconazole as an Additive in Optisol GS A Preservation Medium for Corneal Donor TissueRitterband, David C MD*; Shah, Mahendra K MS†; Meskin, Seth W MD*; Seedor, John A MD*; Koplin, Richard S MD*; Perez, Wilma BS†; Yang, Renee MD*; Hu, Dan Ning MD†; Dahl, Patricia BS‡Author Information From the *Department of Ophthalmology and †Department of Pathology and Laboratory Medicine, The New York Eye & Ear Infirmary, New York, NY, and New York Medical College, Valhalla, NY; and ‡The Eye Bank for Sight Restoration New York, NY. Received for publication July 27, 2006; revision received October 18, 2006; accepted October 25, 2006. Presented in part at The Association for Research in Vision and Ophthalmology (ARVO) Annual Meeting, April 30-May 4, 2006, Ft. Lauderdale, FL. The authors state that they have no proprietary interest in the products named in this article. Reprints: David C. Ritterband, Ophthalmic Consultants PC, The New York Eye and Ear Infirmary, 310 E. 14th Street, New York, NY 10003 (e-mail: [email protected]). Cornea: April 2007 - Volume 26 - Issue 3 - p 343-347 doi: 10.1097/ICO.0b013e31802d82e8 Buy Metrics Abstract Purpose: To assess the endothelial toxicity and the microbiological efficacy of voriconazole (100 μg/mL) as an antimicrobial additive to Optisol GS. Methods: A total of 533 donor rims were studied. One half of each donor rim was placed in standard Optisol GS and the other half rim in Optisol GS fortified with voriconazole (100 μg/mL). All rims were refrigerated for 24 hours at 3°C and placed in thioglycolate broth and incubated at 37°C for 7 days. A pair of donor buttons not used in transplantation was stored for 2 days in each solution and examined for endothelial changes with electron microscopy (EM). A second pair of cornea buttons was examined for toxicity by endothelial staining with 0.3% trypan blue and 0.2% alizarin red. Results: Seven of 533 corneal rim cultures were positive for fungal organisms in the Optisol GS group. No rims were positive for fungal growth in the voriconazole-fortified Optisol GS medium. The difference was statistically significant (P = 0.015; Fisher exact test). There was no difference in the cellular morphology of the button stored in voriconazole fortified Optisol GS compared with Optisol GS using EM. In the bioassay, the percentage of nonviable cells in the voriconazole-fortified medium compared with the control medium was nonsignificant (P < 0.05, Student t test). Conclusions: Voriconazole seems to be safe as a fortifying agent for cornea storage medium. It significantly reduces the rate of positive fungal rim cultures and shows no signs of endothelial cytotoxicity as viewed by EM and by a bioassay of trypan blue and alizarin red. © 2007 Lippincott Williams & Wilkins, Inc.