SYMPOSIUM 1Anatomy and Histopathology of Human Meibomian GlandObata, Hiroto M.D., Ph.D.Author Information Department of Ophthalmology, Jichi Medical School, Tochigi, Japan. Address correspondence and reprint requests to Dr. H. Obata, Department of Ophthalmology, Jichi Medical School, Minamikawachi-machi, Kawachi-gun, Tochigi 329-0498, Japan. E-mail: [email protected] Submitted April 26, 2002. Accepted June 28, 2002. Cornea: October 2002 - Volume 21 - Issue - p S70-S74 doi: 10.1097/01.ico.0000263122.45898.09 Buy Metrics Abstract Purpose To investigate histopathologic changes in human meibomian gland. Methods Human meibomian gland samples were obtained at autopsy from 50 men and 33 women aged from 17 to 87 years with a mean age (±SD) of 61±13 years. Pieces of tarsal plate measuring 3×3 mm including meibomian glands were excised from the center of both upper eyelids, then fixed and embedded in paraffin. Sections 4μ-pm thick were stained with hematoxylin and eosin and periodic acid-Schiff. Light microscopy was used to observe any histopathologic changes. Results The following histopathologic changes were observed: (1) cystic dilatation of acini and/or ducts, (2) atrophy of acini, (3) basement membrane thickening of acini, (4) granulation tissue, and (5) lipogranulomatous inflammation. Conclusion Various histopathologic changes were observed in the human meibomian gland. Hyperkeratinization of ductal epithelium and atrophy of acinar cells may cause meibomian gland dysfunction. © 2002 Lippincott Williams & Wilkins, Inc.