Original Articles: PDF OnlyAntonios Sobhi R. M.D.; Cameron, James A. M.D., F.R.C.S(C); Badr, Ihsan A. F.R.C.S; Habash, Nadim R. M.D., F.R.C.S.(C); Cotter, John B. M.D.Cornea: May 1991 - p 217-220 Free Abstract Nine cases (0.41%) of bacterial and fungal endophthalmitis developed out of a total of 2,210 consecutive penetrating keratoplasties performed between November 1983 and April 1989. Five of the nine cases (0.23%) had endophthalmitis related to donor tissue contamination. The donor tissue of these cases had a storage time of>5 days. A retrospective analysis of 1,399 consecutive corneoscleral rim cultures showed a contamination rate of 29%. The most common organisms isolated were Propionibacterium 26%, diphtheroids 24%, Staphylococcus epidermidis 22%, and fungi 9%. There is a statistically significant increase (p < 0.005) in the percentage of contaminated donor rims with a preservation time of more than five days. The risk of developing endophthalmitis is 12 times greater with a positive donor rim culture. Prolonged preservation of donor tissue can be a risk factor in developing endophthalmitis. © Lippincott-Raven Publishers.