RENAL IMMUNOLOGY AND PATHOLOGY: Edited by Agnes FogoMass spectrometry based proteomics in the diagnosis of kidney diseaseSethi, Sanjeev; Vrana, Julie A.; Theis, Jason D.; Dogan, AhmetAuthor Information Division of Anatomic Pathology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA Correspondence to Sanjeev Sethi, MD, PhD, Department of Laboratory Medicine and Pathology, Mayo Clinic, 200 1st Street SW, Rochester, MN 55905, USA. Tel: +1 507 538 1414; fax: +1 507 284 1875; e-mail: [email protected] Current Opinion in Nephrology and Hypertension: May 2013 - Volume 22 - Issue 3 - p 273-280 doi: 10.1097/MNH.0b013e32835fe37c Buy Metrics Abstract Purpose of review Laser microdissection (LMD) and mass spectrometry (MS) is a new technique that consists of dissection of glomeruli, tryptic digestion of dissected material, analysis by MS and generation of a protein profile using different algorithms. The review focuses on the use of this methodology as an ancillary technique in a clinical laboratory for the diagnosis of kidney diseases. Recent findings LMD/MS is used in the diagnosis and typing of kidney diseases with organized deposits such as amyloidosis. Uncommon and familial forms of renal amyloidosis are diagnosed and typed on the basis of the presence of specific amyloidogenic proteins. LMD/MS is used to confirm and identify immunoglobulins and complement factors in immune complex mediated and complement-mediated proliferative glomerulonephritis, respectively. In particular, LMD/MS can detect monoclonal immunoglobulins in cases of equivocal immunofluorescence studies in monoclonal immunoglobulins-associated glomerulonephritis. LMD/MS can detect specific complement factors of the alternative pathway and terminal pathway in complement-mediated glomerulonephritis. Summary LMD/MS is currently used for diagnosis and typing of amyloidosis. In addition, LMD/MS is useful in determining the type of immunoglobulins and complement factors in immune complex and complement-mediated glomerulonephritis, respectively. © 2013 Lippincott Williams & Wilkins, Inc.