Lessons learned from human HIV vaccine trials : Current Opinion in HIV and AIDS

Secondary Logo

Journal Logo


Lessons learned from human HIV vaccine trials

Pollara, Justina; Easterhoff, Davidb; Fouda, Genevieve G.b

Author Information
Current Opinion in HIV and AIDS 12(3):p 216-221, May 2017. | DOI: 10.1097/COH.0000000000000362
  • Open



Despite recent advances in HIV-1-prevention strategies, there remain over 2 million new HIV-1 infections each year [1–4]. Therefore, an effective HIV vaccine is needed in order to abrogate new infections and reach the target of ending the global AIDS epidemic by the year 2030. There have been six human HIV-vaccine efficacy trials conducted to date [5]. Only one – the RV144 Thai trial – has demonstrated any evidence of vaccine-mediated protection, with a modest estimated vaccine efficacy of 31% [6]. Similar to most licensed vaccines [7], a comprehensive analysis of the immune correlates of reduced infection risk in the RV144 trial identified multiple aspects of vaccine-induced humoral immune responses as contributing to reduced risk of infection [5,8–10,11▪]. Unexpectedly, non-neutralizing antibodies capable of mediating Fc-dependent antiviral effector functions were identified as a correlate of reduced infection risk (see review by C. Moog [12]) [8,13]. The limited success of the RV144 trial demonstrated the proof of principle that vaccination can impart protection from HIV-1 infection, and provides a foundation for development of the next generation of candidate vaccines designed for improved effectiveness in diverse and higher-risk populations. Passive protection studies conducted in nonhuman primate model systems have provided evidence that broadly neutralizing antibodies (bNAbs), defined as those capable of neutralizing multiple difficult to neutralize (tier 2 [14]) HIV-1 primary isolates, are highly effective in preventing infection [15▪,16,17]. These results suggest that inducing bNAb responses by vaccination will be required to improve upon the results of the RV144 clinical trial and to develop a highly effective global HIV vaccine.

Although bNAb responses have not yet been observed in human HIV-1 vaccine trials, recent data from the RV144 and RV305 clinical trials suggest that antibody lineages with long third heavy chain complementarity determining regions (HCDR3s), a characteristic associated with some bNAb lineages [18], were initiated by the vaccine regimen [19]. In this review, we summarize studies of antibody effector functions that have been induced to date with experimental HIV vaccines, and speculate on what may be needed to achieve improved vaccine efficacy.

Box 1:
no caption available


There have been six HIV-vaccine efficacy trials [5,11▪], and a recent search of clinicaltrials.gov (search term: HIV vaccine; intervention: biological; search date: 2 November 2016) identified over 400 completed human clinical studies of candidate HIV vaccines. A general overview of these studies indicated the evaluation of diverse assortments of vaccine regimens, vectors, routes of vaccine administration, adjuvants, immunogens, and study populations (many strategies recently reviewed in [10]). These clinical trials were themselves preceded and informed by preclinical studies in various animal models, further multiplying the number of studies and varieties of approaches that have been conducted in search of an effective vaccine to prevent HIV. Despite this robust and earnest effort, there have been no publications describing induction of HIV-1 antibody responses capable of broad neutralization by a human vaccine trial. The VAX004 trial provided the only evidence of tier 2 virus neutralization, but at low titer and only in a subset of vaccine recipients [20]. Thus, from the human HIV vaccine trials conducted to date, detectable bNAb responses are difficult to induce by vaccination. Nonetheless, careful analysis of these unsuccessful efforts and of bNAbs generated during natural infection is necessary to inform vaccine design.


The neutralizing breadth observed for plasma or serum antibodies generated during natural infection has been studied by isolation of individual monoclonal antibodies (mAbs). bNAbs can be detected in up to 50% of HIV-1-infected individuals, but these responses are subdominant to those lacking neutralizing breadth and develop only after several years of exposure to replicating virus [21–25]. In rare cases, HIV-infected individuals can develop a dominant bNAb response with the ability to overcome the vast genetic diversity and glycan-shielding that characterize the HIV-1 envelope protein (Env) [26,27,28▪▪]. Antibodies with the greatest breadth target conserved regions of neutralization vulnerability on the HIV envelope trimer: the CD4-binding site (CD4bs), the variable region 1 and 2 (V1/V2) glycan, the variable region 3 (V3) glycan, the gp120–gp140 interface, and the gp41 membrane proximal external region [29▪▪]. Significant advances have recently been made in identifying common immunogenetic characteristics of bNAbs – extensive somatic hypermutation, polyreactivity or autoreactivity, and atypically long heavy chain complementarity determining region 3 (HCDR3) or short CDRL3s – all of which are normally restricted by immune tolerance mechanisms and therefore uncommon in the B-cell repertoire [21,29▪▪]. The collective observations from natural HIV infection (reviewed in more detail by A. McKnight [30]) illuminate the underlying reasons why human HIV vaccine trials have yet to induce bNAb responses.


Given the slow development, rarity, and subdominance of bNAb responses in natural HIV-1 infection, it is unsurprising that vaccine-recipient plasma or serum samples with broad HIV-1-neutralizing activity have not yet been induced by candidate vaccines. However, this observation is not sufficient to rule out the possibility that subdominant bNAb responses were induced, or more likely, whether the vaccine has stimulated expansion of B cells that produce bNAb precursors. Detailed characterization of the vaccine-induced B-cell response is critical for identifying strategies that could be used to further drive maturation and expansion of early bNAb responses through subsequent vaccination. The techniques required to identify early potential bNAb lineages or subdominant responses in vaccine recipients include antigen-specific B-cell isolation or total B-cell isolation for memory B-cell culture, sequencing and deep sequencing of immunoglobulin heavy and light chain genes, and production of mAbs by recombinant production methods. These techniques are resource and labor-expensive, and require considerable time and expertise (contribution of D. Corti [31]). Thus, practical constraints have limited the widespread deployment of this level of analysis to only a subset of human vaccine trials, and to only a small subset of vaccine recipients within these trials. Regardless of these limitations, application of B-cell repertoire analysis to vaccine recipients enrolled in the RV144 and RV305 clinical trials has provided new insight into the antigen-specific B-cell responses.


The ALVAC-HIV (vCP1521) prime AIDSVAX B/E gp120 boost vaccine regimen used in the RV144 HIV-1 vaccine trial reduced HIV-1 acquisition risk with an estimated vaccine efficacy of 60% at 12 months [32] and 31% at 42 months [6]. Risk of infection correlated inversely with plasma IgG antibody binding of Env V1/V2, and directly with plasma Env-specific IgA levels [8,33]. Neither the polyclonal plasma, nor mAbs isolated from RV144 vaccine recipients had neutralization activity beyond tier 1 viruses. The impact of additional vaccine boosts on the quality and quantity of the vaccine-induced humoral response was evaluated in the follow-up study, RV305.

The RV305 HIV-1 clinical trial was a delayed (6–8 years later) and repetitive boosting of RV144 vaccine -recipients with the same immunogens that were used in the RV144 vaccine regimen. In the primary plasma analysis of RV305, like in the RV144 trial, no heterologous tier 2 neutralizing activity was detected [34,35]. Whereas Env-specific antibody responses rapidly waned after completion of the RV144 vaccine- regimen [36], a longitudinal interrogation of the post-RV144 and post-RV305 vaccine-induced memory B-cell repertoires found that B-cell clonal lineages started in the RV144 vaccine trial could be boosted many years later [19]. These data suggested that even though plasma Env antibody titers were not sustained at high levels, Env vaccination did induce long-lived memory B cells, and possibly memory CD4+ T cells, that can be recalled with boosting [37,38▪].

The CD4bs, V2-glycan, and V3-glycan bNAbs frequently have a long HCDR3 [18,39,40]. Compared to the post-RV144 Env-specific memory B cells, after RV305 there was a substantial increase in Env-reactive antibodies with long HCDR3s [19]. A subset of the long HCDR3 antibodies isolated after RV305 were CD4bs antibodies with an epitope that overlapped the CD4bs bNAb B12 (Easterhoff et al., [41▪▪]). Structural analysis of one of these antibodies found preferential binding to open Env trimers, thus limiting their ability to neutralize more difficult-to-neutralize isolates with closed trimers. Whereas the CD4bs antibodies isolated after RV305 were not bNAbs, these data reiterate that immunizing with Env protein induces a spectrum of responses from easy-to-induce dominant responses to more difficult-to-induce subdominant responses, and that repetitive boosting with the same immunogen can expand subdominant responses. Given that most HIV-1 vaccine trials have abbreviated vaccine regimens and contain a restricted Env sequence diversity compared to natural infection, it is unlikely that if and when a bNAb precursor is induced, there will be broad and potent neutralizing activity present in the plasma. The only way to determine if the vaccine-induced memory B-cell responses contain antibodies that target a vulnerable site on the virion and have the capacity to develop neutralization breadth and potency is by a deep and careful interrogation of the Env-reactive B-cell repertoire.


Although it is not yet possible to induce bNAb responses with vaccination, antibodies with limited neutralization breadth and with non-neutralizing Fc receptor (FcR)-mediated effector functions can be elicited. These types of antibodies have been correlated with reduced risk of infection during vertical transmission [42,43], in nonhuman primate challenge studies [44], and in the RV144 clinical trial [8,45,46]. In RV144, two immune correlates of decreased transmission risk were identified: high levels of V1/V2 binding antibodies, with subsequent analysis demonstrating a key role for antibodies specific to a linear V2 epitope centered on the lysine at amino acid position 169 (K169) in providing immune pressure that selected for virus escape [13,46]; and increased antibody-dependent cell-mediated cytotoxicity (ADCC) activity in the presence of low circulating Env-specific IgA [8,11▪]. Correlating with these observations, an unbiased analysis of the vaccine-induced memory B-cell repertoire identified V2-specific K169-dependent linear peptide-binding antibodies [46], and antibodies that target conformation epitopes in the first conserved region (C1). The C1 antibodies preferentially used the VH1 gene family, and constituted the major component of the ADCC response [45]. The isolated V2 mAbs are presumed to be representative of the class of antibodies associated with reduced infection risk, and were demonstrated to be capable of multiple antiviral functions including autologous tier 1 neutralization, ADCC activity against tier 2 virus infected cells, and the ability to capture infectious virus [46]. The levels of circulating V2 antibodies in vaccine recipients were not high enough to be effective based on in-vitro testing, but it was demonstrated that the anti-C1 and V2 antibodies could synergize to mediate ADCC at levels compatible with those present in vaccinees [47]. In contrast, a RV144 vaccine-induced IgA mAb was able to block the ADCC activity of vaccine-induced IgG antibodies [33]. Collectively, these observations generated the hypothesis that Fc-dependent immune functions including ADCC likely contributed to the protection observed for RV144, and high levels of IgA may have limited the vaccine efficacy through competition with beneficial antiviral functions of IgG.

The results of the RV144 correlates study highlighted the potential utility of non-neutralizing antibodies in prevention of HIV, leading to interest in exploring ADCC antibodies as immunotherapeutics for treatment and possible cure of HIV infection. Non-neutralizing antibodies including the C1 mAb A32 have recently been engineered into bi-specific antibody-based molecules that allow redirection of endogenous polyclonal CD8+ T cells for potent killing of HIV-infected cells and reactivated latently infected cells [48,49]. A phase I clinical trial is being planned to evaluate one of these bi-specific molecules in HIV-infected humans, and other novel therapeutics based both on ADCC antibodies and bNAbs are currently being tested in preclinical studies [50▪▪].

Collectively, the data from RV144 suggest that in absence of bNAbs, protection can be mediated by a polyclonal and polyfunctional antibody response. However, the RV144 study was conducted in a cohort with low risk of HIV infection [51]. It is unclear if the efficacy observed for RV144 would translate to higher-risk populations. Results from a high-dose mucosal challenge study conducted in nonhuman primates failed to demonstrate protection by an ADCC antibody, although the number of founder viruses that established infection were reduced compared to controls [52]. In a recent vertical transmission study, ADCC activity of passively acquired antibodies did not correlated with transmission risk, but higher ADCC was associated with reduced risk of infant mortality [53]. These data suggest that a vaccine with efficacy beyond that observed for RV144, and effective in higher-risk groups, will likely need to induce both neutralizing and non-neutralizing antibody responses working in concert to prevent infection, and possibly to control viremia and limit disease progression in the event of a breakthrough infection (contributions of C. Moog [12] and R. Ruprecht [54]).


Inducing bNAbs will likely require novel immunization strategies that selectively recapitulate the immunological milieu in chronic HIV-1 infection. Potential strategies being explored include the use of novel immunogens based on structural understanding of the native envelope trimer and soluble stable trimers [55–59] (contribution of M. Ramirez [60]), B-cell lineage immunogen design [61,62], use of novel adjuvants (see review by J. McElrath [63]) to induce robust T-follicular helper cell responses [64,65▪▪], and the inclusion of immunomodulators to transiently inhibit immune tolerance checkpoints in order to overcome constraints that limit the development of polyreactive or autoreactive bNAbs [29▪▪]. Many of these candidate strategies will require several immunizations over an extended period of time. Therefore, immunization in infancy could be an attractive strategy to achieve durable broad anti-HIV neutralizing antibody responses prior to sexual debut. Although it is generally assumed that the developing infant immune system responds poorly to vaccination, recent studies have demonstrated that infants can mount robust responses following HIV vaccination [66▪]. Moreover, recent studies have indicated that young children can develop bNAb responses earlier than adults, and with neutralization breadth comparable to the top 1% of adult neutralizers [67]. Similarly, Adland et al.[68] measured neutralizing antibody responses in HIV-1-infected children and found that 70% of infant slow progressors, but only 15% of HIV-1 clade C chronically infected adults, were able to neutralize at least 50% of viruses tested. Children also had higher neutralization titers than adults. Importantly, Simonich et al.[69▪] reported that a broad neutralizing mAb isolated from a HIV-infected child has lower levels of mutation when compared to adult bNAbs, further suggesting that it could be easier to induce bNAbs in children than in adults. It is therefore critical to test novel immunization strategies in pediatric populations.


All the HIV vaccine trials conducted to date, including the moderately efficacious RV144 Thai trial, failed to induce plasma bNAb responses. Recent analysis of the vaccine-induced memory B-cell repertoire in RV144/305 vaccine recipients demonstrated that repetitive immunization can shift the Env-specific repertoire expanding subdominant populations of B cells. These data highlight the importance of analyzing vaccine-elicited antibody responses beyond plasma. A thorough analysis of vaccine-elicited responses at the B-cell repertoire level is crucial to determine if a candidate vaccine was able to recruit subdominant B-cell lineages with characteristics of bNAbs, and to inform rational design of a boosting immunogen to select for B-cell clonal lineage members with increased neutralization breadth. Ultimately, an effective HIV vaccine will likely need to elicit a diverse antibody response that includes bNAbs and non-neutralizing antibodies with FcR-mediated antiviral effector functions.


We thank Drs Barton F. Haynes, Georgia D. Tomaras, Sallie R. Permar, and Guido Ferrari for helpful discussion in preparation of the manuscript.

Financial support and sponsorship

The study was supported by the Center for HIV/AIDS Vaccine Immunology-Immunogen Discovery (CHAVI-ID; UM1-AI100645), the Duke University Center for AIDS Research (CFAR; NIH 5P30 AI064518), the University of North Carolina Collaboratory of AIDS Researchers for Eradication (CARE; U19 A1096113), by NIH Grants R21 AI127022 and R03 HD085871–01, Duke CTSA KL2 career development award (KL2TR001115), Duke Surgery Clarence E. Gardner award, and Collaboration for AIDS Vaccine Discovery grants from the Bill and Melinda Gates Foundation.

Conflicts of interest

There are no conflicts of interest.


Papers of particular interest, published within the annual period of review, have been highlighted as:

  • ▪ of special interest
  • ▪▪ of outstanding interest


1. Joint United Nations Program on HIV/AIDS. Global AIDS update 2016. Switzerland: UNAIDS; 2016.
2. World Health Organization. Guideline On When To Start Antiretroviral Therapy and On Preexposure Prophylaxis For HIV. WHO Guidelines Approved by the Guidelines Review Committee. Geneva: WHO; 2015.
3. Davies O, Ustianowski A, Fox J. Preexposure prophylaxis for HIV prevention: why, what, who and how. Infect Dis Ther 2016; 5:407–416.
4. UNAIDS, Joint United Nations Program on HIV/AIDS. 2015 Progress report on the global plan towards the elimination of new HIV infections among children and keeping their mothers alive. 2015.
5. Excler JL, Michael NL. Lessons from HIV-1 vaccine efficacy trials. Curr Opin HIV AIDS 2016; 11:607–613.
6. Rerks-Ngarm S, Pitisuttithum P, Nitayaphan S, et al. Vaccination with ALVAC and AIDSVAX to prevent HIV-1 infection in Thailand. N Engl J Med 2009; 361:2209–2220.
7. Plotkin SA. Correlates of protection induced by vaccination. Clin Vaccine Immunol 2010; 17:1055–1065.
8. Haynes BF, Gilbert PB, McElrath MJ, et al. Immune-correlates analysis of an HIV-1 vaccine efficacy trial. N Engl J Med 2012; 366:1275–1286.
9. Corey L, Gilbert PB, Tomaras GD, et al. Immune correlates of vaccine protection against HIV-1 acquisition. Sci Transl Med 2015; 7:310rv317.
10. Excler JL, Tomaras GD, Russell ND. Novel directions in HIV-1 vaccines revealed from clinical trials. Curr Opin HIV AIDS 2013; 8:421–431.
11▪. Tomaras GD, Haynes BF. Advancing toward HIV-1 vaccine efficacy through the intersections of immune correlates. Vaccines (Basel) 2014; 2:15–35.

This study comprehensively reviews the immune correlates of infection risk identified in human efficacy trials.

12. Mayr L, Su B, Moog C. Role of non neutralizing antibodies in vaccines and/or HIV infected individual. Curr Opin HIV AIDS 2017; 12:209–215.
13. Rolland M, Edlefsen PT, Larsen BB, et al. Increased HIV-1 vaccine efficacy against viruses with genetic signatures in Env V2. Nature 2012; 490:417–420.
14. Seaman MS, Janes H, Hawkins N, et al. Tiered categorization of a diverse panel of HIV-1 Env pseudoviruses for assessment of neutralizing antibodies. J Virol 2010; 84:1439–1452.
15▪. Gautam R, Nishimura Y, Pegu A, et al. A single injection of anti-HIV-1 antibodies protects against repeated SHIV challenges. Nature 2016; 533:105–109.

This study demonstrates the efficacy of bNAbs in prevention of infection following passive infusion. A single administration of bNAbs protected nonhuman primates against repeated intrarectal challenge for up to 23 weeks, supporting the continued effort to develop an HIV vaccine that can elicit these types of antibody responses.

16. Moldt B, Rakasz EG, Schultz N, et al. Highly potent HIV-specific antibody neutralization in vitro translates into effective protection against mucosal SHIV challenge in vivo. Proc Natl Acad Sci U S A 2012; 109:18921–18925.
17. Yamamoto H, Matano T. Patterns of HIV/SIV prevention and control by passive antibody immunization. Front Microbiol 2016; 7:1739.
18. Mascola JR, Haynes BF. HIV-1 neutralizing antibodies: understanding nature's pathways. Immunol Rev 2013; 254:225–244.
19. Moody M, Easterhoff D, Gurley T, et al. Induction of antibodies with long variable heavy third complementaryity determining regions by repetitive boosting with AIDSVAX B/E in RV144 vaccinees. HIV R4P 2014; OA12.06; Cape Town, South Africa.
20. Gilbert P, Wang M, Wrin T, et al. Magnitude and breadth of a nonprotective neutralizing antibody response in an efficacy trial of a candidate HIV-1 gp120 vaccine. J Infect Dis 2010; 202:595–605.
21. Burton DR, Mascola JR. Antibody responses to envelope glycoproteins in HIV-1 infection. Nat Immunol 2015; 16:571–576.
22. Hraber P, Seaman MS, Bailer RT, et al. Prevalence of broadly neutralizing antibody responses during chronic HIV-1 infection. AIDS 2014; 28:163–169.
23. Gray ES, Madiga MC, Hermanus T, et al. The neutralization breadth of HIV-1 develops incrementally over four years and is associated with CD4+ T cell decline and high viral load during acute infection. J Virol 2011; 85:4828–4840.
24. Landais E, Huang X, Havenar-Daughton C, et al. Broadly neutralizing antibody responses in a large longitudinal sub-Saharan HIV primary infection cohort. PLoS Pathog 2016; 12:e1005369.
25. Sather DN, Armann J, Ching LK, et al. Factors associated with the development of cross-reactive neutralizing antibodies during human immunodeficiency virus type 1 infection. J Virol 2009; 83:757–769.
26. Scanlan CN, Offer J, Zitzmann N, et al. Exploiting the defensive sugars of HIV-1 for drug and vaccine design. Nature 2007; 446:1038–1045.
27. Simek MD, Rida W, Priddy FH, et al. Human immunodeficiency virus type 1 elite neutralizers: individuals with broad and potent neutralizing activity identified by using a high-throughput neutralization assay together with an analytical selection algorithm. J Virol 2009; 83:7337–7348.
28▪▪. Rusert P, Kouyos RD, Kadelka C, et al. Determinants of HIV-1 broadly neutralizing antibody induction. Nat Med 2016; 22:1260–1267.

A detailed study of the virus and host factors that drive development of bNAb responses in a large study cohort. Specifically, the study evaluated the role of viral load, CD4 level, duration of infection, HIV pol subtype, patient ethnicity, and patient sex for association with induction of bNAbs.

29▪▪. Haynes BF, Shaw GM, Korber B, et al. HIV-host interactions: implications for vaccine design. Cell Host Microbe 2016; 19:292–303.

A comprehensive review of the co-evolution of the transmitted virus and the host response, and how this knowledge is being used to develop novel strategies for HIV vaccine design.

30. McCoy LE, McKnight Á. Lessons learned from humoral responses of HIV patients. Curr Opin HIV AIDS 2017; 12:195–202.
31. Corti D. HT generation of mABs from HIV infected Individuals - Game changer. Curr Opin HIV AIDS 2017; 12:000.
32. Robb ML, Rerks-Ngarm S, Nitayaphan S, et al. Risk behaviour and time as covariates for efficacy of the HIV vaccine regimen ALVAC-HIV (vCP1521) and AIDSVAX B/E: a posthoc analysis of the Thai phase 3 efficacy trial RV 144. Lancet Infect Dis 2012; 12:531–537.
33. Tomaras GD, Ferrari G, Shen X, et al. Vaccine-induced plasma IgA specific for the C1 region of the HIV-1 envelope blocks binding and effector function of IgG. Proc Natl Acad Sci U S A 2013; 110:9019–9024.
34. Karasavvas N, Karnasuta C, Ngauy V, et al. Investigation of antibody responses induced in RV305 a late boost vaccination of HIV-1 uninfected volunteers that participated in RV144, a Thai trial. AIDS Vaccine 2013; P03.68LB; Barcelona, Spain.
35. Akapirat S, Karnasuta C, Madnote S, Savadsuk H, et al. HIV-specific antibody in rectal secretions following late boosts in RV144 participants (RV305). HIV R4P 2014; OA11.05; Cape Town, South Africa.
36. Yates NL, Liao HX, Fong Y, et al. Vaccine-induced Env V1-V2 IgG3 correlates with lower HIV-1 infection risk and declines soon after vaccination. Sci Transl Med 2014; 6:228ra239.
37. Moody MA, Yates NL, Amos JD, et al. HIV-1 gp120 vaccine induces affinity maturation in both new and persistent antibody clonal lineages. J Virol 2012; 86:7496–7507.
38▪. Wang Y, Sundling C, Wilson R, et al. High-resolution longitudinal study of HIV-1 Env vaccine-elicited B cell responses to the virus primary receptor binding site reveals affinity maturation and clonal persistence. J Immunol 2016; 196:3729–3743.

This study uses B-cell repertoire analysis to characterize the affinity maturation of CD4bs-lineage antibodies in a preclinical model.

39. Tiller T, Tsuiji M, Yurasov S, et al. Autoreactivity in human IgG+ memory B cells. Immunity 2007; 26:205–213.
40. Wu TT, Johnson G, Kabat EA. Length distribution of CDRH3 in antibodies. Proteins 1993; 16:1–7.
41▪▪. Easterhoff D, Moody MA, Ferra D, et al. Boosting of HIV envelope CD4 binding site antibodies with long variable heavy third complementarity determining region in the randomized double blind RV305 HIV-1 vaccine trial. PLoS Pathogens. In press.

Memory B cell repertoire analysis was used to define a set of CD4 binding site reactive B cell clonal lineages that were initiated by the RV144 vaccine-regimen and expanded by vaccine-boosting in the RV305 trial.

42. Mabuka J, Nduati R, Odem-Davis K, et al. HIV-specific antibodies capable of ADCC are common in breastmilk and are associated with reduced risk of transmission in women with high viral loads. PLoS Pathog 2012; 8:e1002739.
43. Permar SR, Fong Y, Vandergrift N, et al. Maternal HIV-1 envelope-specific antibody responses and reduced risk of perinatal transmission. J Clin Invest 2015; 125:2702–2706.
44. Gordon SN, Liyanage NP, Doster MN, et al. Boosting of ALVAC-SIV vaccine-primed macaques with the CD4-SIVgp120 fusion protein elicits antibodies to V2 associated with a decreased risk of SIVmac251 acquisition. J Immunol 2016; 197:2726–2737.
45. Bonsignori M, Pollara J, Moody MA, et al. Antibody-dependent cellular cytotoxicity-mediating antibodies from an HIV-1 vaccine efficacy trial target multiple epitopes and preferentially use the VH1 gene family. J Virol 2012; 86:11521–11532.
46. Liao HX, Bonsignori M, Alam SM, et al. Vaccine induction of antibodies against a structurally heterogeneous site of immune pressure within HIV-1 envelope protein variable regions 1 and 2. Immunity 2013; 38:176–186.
47. Pollara J, Bonsignori M, Moody MA, et al. HIV-1 vaccine-induced C1 and V2 Env-specific antibodies synergize for increased antiviral activities. J Virol 2014; 88:7715–7726.
48. Ferrari G, Pollara J, Kozink D, et al. An HIV-1 gp120 envelope human monoclonal antibody that recognizes a C1 conformational epitope mediates potent antibody-dependent cellular cytotoxicity (ADCC) activity and defines a common ADCC epitope in human HIV-1 serum. J Virol 2011; 85:7029–7036.
49. Sung JA, Pickeral J, Liu L, et al. Dual-affinity re-targeting proteins direct T cell-mediated cytolysis of latently HIV-infected cells. J Clin Invest 2015; 125:4077–4090.
50▪▪. Ferrari G, Haynes BF, Koenig S, et al. Envelope-specific antibodies and antibody-derived molecules for treating and curing HIV infection. Nat Rev Drug Discov 2016; 15:823–834.

This review discusses antibody-based immunotherapies under evaluation for treatment and cure of HIV infection.

51. Ministry of Public Health-Thai AVEG. Screening and evaluation of potential volunteers for a phase III trial in Thailand of a candidate preventive HIV vaccine (RV148). Vaccine 2011; 29:4285–4292.
52. Santra S, Tomaras GD, Warrier R, et al. Human nonneutralizing HIV-1 envelope monoclonal antibodies limit the number of founder viruses during SHIV mucosal infection in rhesus macaques. PLoS Pathog 2015; 11:e1005042.
53. Milligan C, Richardson BA, John-Stewart G, et al. Passively acquired antibody-dependent cellular cytotoxicity (ADCC) activity in HIV-infected infants is associated with reduced mortality. Cell Host Microbe 2015; 17:500–506.
54. Ruprecht RM, Lakhashe SK. Antibody-mediated immune exclusion of HIV. Curr Opin HIV AIDS 2017; 12:222–228.
55. de Taeye SW, Ozorowski G, Torrents de la Pena A, et al. Immunogenicity of stabilized HIV-1 envelope trimers with reduced exposure of nonneutralizing epitopes. Cell 2015; 163:1702–1715.
56. Derking R, Ozorowski G, Sliepen K, et al. Comprehensive antigenic map of a cleaved soluble HIV-1 envelope trimer. PLoS Pathog 2015; 11:e1004767.
57. Julien JP, Lee JH, Ozorowski G, et al. Design and structure of two HIV-1 clade C SOSIP.664 trimers that increase the arsenal of native-like Env immunogens. Proc Natl Acad Sci U S A 2015; 112:11947–11952.
58. Sanders RW, Derking R, Cupo A, et al. A next-generation cleaved, soluble HIV-1 Env trimer, BG505 SOSIP.664 gp140, expresses multiple epitopes for broadly neutralizing but not nonneutralizing antibodies. PLoS Pathog 2013; 9:e1003618.
59. Ward AB, Wilson IA. Insights into the trimeric HIV-1 envelope glycoprotein structure. Trends Biochem Sci 2015; 40:101–107.
60. Medina-Ramı’rez M, Sanders RW, Sattentau QJ. Stabilized HIV-1 envelope glycoprotein trimers for vaccine use. Curr Opin HIV AIDS 2017; 12:241–249.
61. Jardine JG, Ota T, Sok D, et al. HIV-1 vaccines priming a broadly neutralizing antibody response to HIV-1 using a germline-targeting immunogen. Science 2015; 349:156–161.
62. Haynes BF, Kelsoe G, Harrison SC, et al. B-cell-lineage immunogen design in vaccine development with HIV-1 as a case study. Nat Biotechnol 2012; 30:423–433.
63. McElrath MJ. Adjuvants tailoring humoral immune responses. Curr Opin HIV AIDS 2017; 12:278–284.
64. Locci M, Havenar-Daughton C, Landais E, et al. Human circulating PD-1+CXCR3-CXCR5+ memory Tfh cells are highly functional and correlate with broadly neutralizing HIV antibody responses. Immunity 2013; 39:758–769.
65▪▪. Moody M, Pedroza-Pacheco I, Vandergrift NA, Chui C. Immune perturbations in HIV-1–infected individuals who make broadly neutralizing antibodies. Sci Immunol 2016; 1:aag0851.

Seminal study describing unique immunological characteristics of individuals who make HIV bNABs.

66▪. Fouda GG, Cunningham CK, McFarland EJ, et al. Infant HIV type 1 gp120 vaccination elicits robust and durable anti-V1V2 immunoglobulin G responses and only rare envelope-specific immunoglobulin A responses. J Infect Dis 2015; 211:508–517.

This study demonstrates the ability of infants to develop a robust and durable antibody response to HIV Env vaccination.

67. Goo L, Chohan V, Nduati R, et al. Early development of broadly neutralizing antibodies in HIV-1-infected infants. Nat Med 2014; 20:655–658.
68. Adland E, Muenchhoff AC, Thumbi PN, Jooste P. Potent broadly neutralising antibody responses in slow-progressing pediatric HIV. CROI 2016; 2016; Boston, Massachusetts.
69▪. Simonich CA, Williams KL, Verkerke HP, et al. HIV-1 Neutralizing Antibodies with Limited Hypermutation from an Infant. Cell 2016; 166:77–87.

Recent description of a bNAb isolated from an HIV-infected infant, with lower levels of mutation compared to most adult bNAbs; suggesting these types of antibodies may be easier to elicit in infants compared to adults and supporting the evaluation of HIV vaccine strategies in pediatric populations.


B-cell repertoire; broadly neutralizing antibodies; vaccine

Copyright © 2017 Wolters Kluwer Health, Inc. All rights reserved.