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Regulation of stress-induced hematopoiesis

Zhao, Jimmy L.a; Baltimore, Davidb

Current Opinion in Hematology: July 2015 - Volume 22 - Issue 4 - p 286–292
doi: 10.1097/MOH.0000000000000149
HEMATOPOIESIS: Edited by Hal E. Broxmeyer
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Purpose of review Hematopoietic stem cells can self-renew and also give rise to the entire repertoire of hematopoietic cells. During acute infectious and inflammatory stresses, the hematopoietic system can quickly adapt to demand by increasing output of innate immune cells many-fold, often at the expense of lymphopoiesis and erythropoiesis. We review recent advances in understanding the regulation of stress-induced hematopoiesis with a specific focus on the direct effects of inflammatory signaling on hematopoietic stem and progenitor cells (HSPCs).

Recent findings Recent studies have highlighted several areas of exciting new developments in the field, including the complex interaction and crosstalk within HSPCs and between bone marrow mesenchymal stem cells and endothelial cells needed to achieve regulated myelopoiesis, identification of increased number of inflammatory and infectious molecules with direct effects on HSPCs, the critical role of inflammatory signaling on embryonic specification of hematopoietic stem cells, and the ability of cytokines to instruct lineage choice at the HSPC level.

Summary These exciting new findings will shape our fundamental understanding of how inflammatory signaling regulates hematopoiesis in health and disease, and facilitate the development of potential interventions to treat hematologic diseases associated with altered inflammatory signaling.

aDavid Geffen School of Medicine, University of California, Los Angeles

bDivision of Biology and Bioengineering, California Institute of Technology, California, USA

Correspondence to Jimmy L. Zhao, 10833 Le Conte Avenue, Los Angeles, CA 90095, USA. E-mail: jzhao@caltech.edu

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INTRODUCTION

During infection and inflammation, a stress-induced hematopoietic response is often elicited, which is characterized by increased output of cells of the myeloid lineage from the bone marrow hematopoietic stem and progenitor cells (HSPCs) and mobilization of bone marrow neutrophils and HSPCs to the peripheral tissues. Similarly, hematopoiesis is shifted toward erythrocyte production during stresses such as hemorrhage and anemia. The remarkable adaptability of the hematopoietic system to meet the demand of various stressed situations is critical for survival. Myeloid cells are mostly postmitotic and need to be produced from bone marrow HSPCs when they are consumed during a fight against infection. How is the increased production of myeloid cells achieved in response to an infection? In general, committed myeloid progenitor cells are induced to proliferate and mature as a rapid immediate response, a process termed ‘emergency granulopoiesis’. This process is covered in a recent comprehensive review [1▪]. However, studies in the recent decade have convincingly shown that uncommitted HSPCs are actively involved in stress-induced hematopoiesis.

HSPCs may respond to an infection via five general mechanisms: (i) respond to the depletion of downstream neutrophils. Although an intuitively attractive hypothesis, how HSPCs can sense and ‘numerate’ peripheral neutrophils remains unclear. There is some evidence that a ‘neutrostat’ system exists to sense the phagocytosis of apoptotic neutrophils consumed during an infection and thus signal the bone marrow to replenish the neutrophil pool accordingly [2,3]. (ii) Respond to inflammatory cytokines produced by various hematopoietic and nonhematopoietic cells during an infection. There is clear evidence that HSPCs, including long-term (LT)-HSCs, have the ability to directly respond to cytokines, including thrombopoietin, IL-3, stem cell factors, Flt3 ligand, interferons (IFNs), tumor necrosis factor alpha (TNFα), tumor growth factor beta (TGFβ), IL-6, granulocyte colony stimulating factor (G-CSF), and macrophage colony stimulating factor (M-CSF). (iii) Respond to pathogen-associated molecular patterns (PAMPs) and danger-associated molecular patterns (DAMPs) directly through toll-like receptors (TLRs). The evidence to support this mechanism is also strong (iv) Respond to paracrine signals from the stem cell niche. Stem cell niche cells, including but not limited to osteoblasts, perivascular stromal cells, and mesenchymal stem cells (MSCs), and endothelial cells are affected in various ways during an infection, which can influence proliferation, differentiation, and mobilization of HSPCs. (v) Theoretically, pathogens can affect HSPC activity by infecting them. However, we are not aware of any convincing evidence to support this. In this review, we will focus our discussion of stress-induced hematopoiesis to mechanisms ii and iii – the direct effects of PAMPs, DAMPs, and cytokines on HSPCs, including LT-HSCs and short-term HSCs and multipotent progenitor cells (MPPs). The important effects of infectious and inflammatory signaling on the bone marrow niche have been the subjects of several recent excellent reviews [4▪▪,5▪▪] and will not be discussed here. We will also provide recent evidence on the pathologic consequences of chronic inflammation on the hematopoietic system, and how we may utilize our knowledge in this area to treat human hematologic malignancies.

Box 1

Box 1

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REGULATION OF STRESS-INDUCED HEMATOPOIESIS BY TOLL-LIKE RECEPTOR SIGNALING

TLRs, belonging to a group of pattern recognition receptors, bind to conserved PAMPs, for example, lipopolysaccharide (LPS) of the bacteria cell wall and single-stranded RNA of viruses, and signal through either MyD88-dependent or TIR-domain-containing adapter-inducing interferon beta (TRIF)-dependent pathways, leading to activation of the nuclear factor kappa light chain enhancer of activated B cells (NF-κB) and/or the interferon regulatory factor (IRF) family of transcription factors [6]. Nagai et al.[7] first demonstrated that HSPCs (defined as Flk2lineageSca1+cKit+ cells) expressed TLR2 and TLR4 receptors; furthermore, stimulation of HSPCs through either TLR2 or TLR4 drove myeloid differentiation in vitro in a MyD88-dependent manner. Soon after, Massberg et al.[8] showed that both bone marrow HSPCs and egressed HSPCs from peripheral lymph differentiated into myeloid cells after implantation under the kidney capsule when stimulated with LPS in vivo. Since then, many studies have confirmed these initial findings [9,10,11▪,12–15]. Furthermore, when highly purified LT-HSCs (lineageSca1+cKit+ CD150+CD48) were stimulated directly with LPS in vitro, NF-κB activation was readily detected using a green fluorescent protein-reporter mouse, demonstrating a functional TLR-4–NF-κB axis in LT-HSCs [11▪]. The consequences of TLR stimulation on HSPCs are many. In-vitro activation of TLRs augments myeloid differentiation [7,11▪]. In-vivo stimulation in mice generally results in increased HSC proliferation, decreased quiescence, skewed myeloid differentiation, and decreased long-term repopulating ability [8–10,13–18]. TLR stimulation of human HSPCs also induces preferentially myeloid differentiation [19,20]. Although TLR4 and TLR2 are among the most commonly studied TLRs in the setting of infection, other TLRs may mediate similarly robust myelopoiesis when challenged with a pathogen. This is highlighted in one study that subjected various genetic knockout mice to Staphylococcus aureus infection or polymicrobial peritonitis and found that stress-induced hematopoiesis did not depend on any single TLRs, cytokines, or interferons, suggesting that a tremendous redundancy has evolved in the mammalian hematopoietic system to sense and respond to bacterial infection [21].

Recent studies by us and others have shed additional insight into the complex crosstalk between hematopoietic and nonhematopoietic cells that corroboratively achieve efficient pathogen detection and subsequent upregulation of myelopoiesis (Fig. 1a). Taking advantage of a microfluidic single cell proteomics platform, we found that a large subset of short-term HSCs and MPPs produced a surprisingly wide range of hematopoietic growth factors and cytokines in response to direct LPS and Pam3CSK4 stimulation [11▪]. This was regulated by the TLR–NF-κB axis, as tuning up or down the strength of NF-κB activity could change the amounts of cytokines produced. Interestingly, the quantity and breadth of cytokines produced by HSPCs trumped those produced by mature myeloid and lymphoid cells by many fold. More importantly, instead of a vestigial feature, HSPC-produced cytokines, especially IL-6, promoted myeloid differentiation in an autocrine or paracrine manner. This was demonstrated in vitro and during neutropenic in-vivo conditions. The short distance autocrine and paracrine communication within HSPCs is likely also functionally important in the bone marrow stem cell niche or extramedullary sites where circulating HSPCs reside. These HSPC-initiated hematopoietic centers may be able to generate a wide range of hematopoietic responses tailored to particular pathogens or other stress signals. Future studies will be needed to address the functional significance of HSPC-produced cytokines in a physiological bone marrow niche in the absence of neutropenia or in an extramedullary site containing egressed HSPCs. In addition to HSPCs as a direct translator of pathogen signals into self-directing myelopoietic cytokine signals, bone marrow MSCs were shown to be involved in promoting myelopoiesis during a viral infection. A recent study nicely demonstrated that in response to antigen stimulation or acute viral infection, cytotoxic T cells released IFNγ, acting on MSCs to produce IL-6. Similarly, IL-6 acted on bone marrow HSPCs to promote myeloid differentiation [22▪]. Given the known effect of IFNγ on HSCs [15], it will be interesting to separate the direct and indirect effects of IFNγ on HSCs and the stem cell niche, respectively, during a viral infection.

FIGURE 1

FIGURE 1

Furthermore, endothelial cells have been shown to be an important sensor of systemic LPS or Escherchia coli challenge, leading to G-CSF production in a MyD88-dependent but TRIF-independent manner [23▪]. In these models, endothelium-produced G-CSF was shown to be the primary stimulator of emergency granulopoiesis. It is interesting to note that, in contrast to LPS injection, E. coli challenge stimulated emergency granulopoiesis, albeit at a reduced level, when MyD88 was deleted in endothelial cells. These suggest that cells other than endothelial cells and cytokines other than G-CSF can function to promote granulopoiesis in a more complex infectious model. In fact, G-CSF has been shown to be dispensable for emergency granulopoiesis in systemic fungal infection [24]. G-CSF signaling regulates granulopoiesis at multiple stages, from uncommitted HSPCs to neutrophils. However, the predominant effect of G-CSF is on lineage-committed granulocytic precursor cells, at least for steady-state granulopoiesis [25,26]. It will be interesting to determine whether G-CSF acts primarily on committed myeloid progenitor cells, bone marrow niche cells, or directly on HSPCs to promote myeloid differentiation during stressed conditions.

Mechanistically, the TLR–MyD88/TRIF–NF-κB axis is among the most commonly implicated pathway in regulating stress-induced hematopoiesis. Interestingly, a recent study showed that LPS induced granyzme B expression in LT-HSCs through the TLR4-TRIF-p65 NF-κB axis and granzyme B caused cell-autonomous apoptosis in HSCs [27▪]. However, LPS induced cytokine production in ST-HSCs and MPPs through the TLR-p50 NF-κB pathway, leading to cytokine production and autocrine/paracrine signaling [11▪]. Suffice it to say that stress-induced hematopoiesis is a highly complex and dynamic process, which involves crosstalk between HSPCs, bone marrow stromal cells, and nonhematopoietic tissues to sense a pathogenic organism and convert the signal of an infection into the signal for myeloid differentiation.

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REGULATION OF STRESS-INDUCED HEMATOPOIESIS BY CYTOKINE SIGNALING

In addition to TLR ligands, cytokines produced during stressed conditions can directly act on HSPCs to regulate hematopoiesis. Stem cell factors, thrombopoietin, Flt3 ligand, IL-3, and IL-6 are among some of the first identified cytokines to regulate HSPC proliferation, self-renewal, and survival [28–33]. For example, we and other groups recently showed that IL-6 can be produced by bone marrow HSPCs during TLR stimulation and by MSCs during IFNγ stimulation and can act on neighboring HSPCs, in particularly MPPs, to stimulate myelopoiesis [11▪,22▪,34]. Recent studies have also expanded the list of cytokines and PAMPs with direct effects on HSPCs (Fig. 1b) [12,15,35,36]. Both IFNα and IFNγ were shown to directly stimulate quiescent HSCs to proliferate [37–40]. More recent evidence suggested that the effects of interferons on HSCs may be complex. Although the finding that IFNγ exposure has a negative impact on HSC's long-term repopulating potential is generally agreed upon, whether IFNγ stimulates or inhibits HSC proliferation has been debated [41,42]. A recent study showed that IFNα-driven HSC proliferation was only transient and HSCs rapidly returned to a quiescent state with chronic exposure, a protective mechanism from chronic infection-driven HSC exhaustion [43▪]. Effects of TNFα on HSCs are also dose and context dependent. In general, it appears that activation of TNF signaling in HSCs inhibits proliferation and results in decreased long-term repopulating potential [44–46]. However, studies using aged mice or different doses of TNFα showed that activation of TNFα signaling in HSCs was protective against apoptosis and thus enhanced hematopoietic engraftment in a transplant setting [47,48]. TGFβ1 appeared to have differential regulatory effects on different subsets of HSCs, stimulating myeloid-biased HSCs to proliferate while inhibiting lymphoid-biased HSCs [49]. Mechanistically, TGFβ1 signaling in HSCs was regulated in part by transcription intermediary factor 1γ (Tif1γ) and RNA binding protein Musashi 2 (Msi-2) [50,51].

Recent years have also seen a renewed debate over whether cytokines can instruct lineage choice of uncommitted HSPCs versus simply promoting preferential proliferation and survival of stochastically committed progenitor cells [52]. Mossadegh-Keller et al. took advantage of time-lapse single cell imaging and single cell gene expression analysis to show that M-CSF, but not G-CSF or GM-CSF, directly induced PU.1 expression in LT-HSCs and instructed LT-HSCs toward myeloid lineage in the absence of proliferative or survival advantage, thus providing a strong case that cytokines can instruct lineage choice in uncommitted HSCs [53▪▪]. Furthermore, a high level of erythropoietin was also shown to instruct HSCs and MPPs towards the erythroid lineage at the expense of myeloid and lymphoid cells by suppressing nonerythroid transcriptional programs in uncommitted stem and progenitor cells [54▪]. These studies underscore the ability of hematopoietic cytokines released under different stressed conditions to actively instruct uncommitted HSPCs to differentiate into a particular cell lineage on demand (Fig. 1c).

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REGULATION OF EMBRYONIC HEMATOPOIETIC STEM CELL SPECIFICATION BY INFLAMMATORY SIGNALING

Another important concept has emerged recently – inflammatory signaling plays a vital role in embryonic HSC specification. Several studies have shown that inflammatory pathways, including TNFα, IFNγ, IFNα, and TLR signaling, serve a surprisingly important role in the emergence of early embryonic HSPCs in mice and zebrafish (Fig. 1d) [55▪–58▪]. Sawamiphak et al.[58▪] found that overexpressing IFNγ increased the number of emerging HSCs from the hemogenic endothelium in zebrafish, while knocking down IFNγ or its receptor had the opposite effect; the authors went on to show that IFNγ likely acted downstream of Notch signaling and blood flow in an cell-autonomous manner through STAT3 to drive HSC emergence. In a parallel study, Espin-Palazon et al.[57▪] examined a different inflammatory pathway involving TNFα in both zebrafish and mice and found that primitive neutrophils were the source of TNFα, which acted through TNFR2 to activate again Notch signaling and NF-κB to promote the emergence of HSCs from hemogenic endothelium. Similar findings were reported almost concurrently in two additional studies. Li et al.[56▪] demonstrated that both IFNγ and IFNα played important roles in HSPC formation in both mice and zebrafish and the source of interferons was also from primitive myeloid population, whereas He et al.[55▪] showed that inflammatory signaling involving the TLR–NF-κB–Notch axis was an important regulator of hemogenic endothelium-derived HSPC development. These studies are intriguing and have opened a new area of investigation in HSC development in the context of inflammatory signaling. Clearly, a conserved set of molecules and signaling pathways involving interferons, TNFα, TLRs, and NF-κB that are traditionally linked to inflammation and infection is also critical in the early embryonic emergence of HSCs. From a more philosophical perspective, how did inflammatory molecules evolve to regulate both embryonic HSC development and stressed hematopoiesis during an infection? It is interesting that mice with genetic deletion in many of these pathways are not only viable but have surprisingly few defects in the hematopoietic stem cell compartment, suggesting that there may be substantial redundancy and/or compensatory mechanism in the signaling pathways for hematopoietic stem cell specification. Perhaps stress is required to reveal the HSC defects in these mice with genetic deletions.

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CONCLUSION

The remarkable ability of HSPCs to adapt to various stresses, such as infection and inflammation, is critical for an organism's survival. In the acute setting, pathogen-associated molecules and inflammatory cytokines generally induce HSPCs to proliferate and differentiate toward the myeloid lineage, serving as a protective immune response against pathogens. However, chronic uncontrolled stimulation may be detrimental to HSC's self-renewal and long-term multilineage differentiation ability. Recent studies have increasingly recognized an active role of HSPCs at the heart of a well-orchestrated hematopoietic response during stressed conditions. However, several major areas of research have not been addressed. First, viral, bacterial, and fungal pathogens often activate multiple TLRs and stimulate production of numerous cytokines. A systems biology approach may be needed to understand how HSPCs integrate different combinations of TLR and cytokine signals into a unique hematopoietic response. Second, the recent discovery that basal activation of inflammatory signaling in the absence of infection promotes embryonic HSC development opens up many areas of research. Can we identify the core inflammatory signaling to aid ex-vivo differentiation of HSCs from embryonic stem cells or induced pluripotent stem cells, one of the major hurdles in regenerative medicine? Is basal activation of TLR or cytokine signaling also important for steady-state hematopoiesis? If so, what are the endogenous sources of TLR ligands and inflammatory cytokines? Finally, how can we apply the knowledge of physiological stress-induced hematopoiesis to human hematologic diseases? One important question is whether dysregulation of stress-induced hematopoiesis contributes to bone marrow failure and hematopoietic malignancies in humans. Interestingly, several large-scale epidemiological studies have shown that chronic immune stimulation from infections and autoimmune diseases increases the risk of developing myeloproliferative neoplasms, bone marrow fibrosis, acute myelogenous leukemia, and myelodysplastic syndrome (MDS) later on [59–61]. Using a genetic mouse model with deficiency in microRNA-146a, we have provided direct experimental evidence that chronic low-grade inflammation in genetic susceptible hosts was sufficient to drive HSC exhaustion, leading to bone marrow failure and myeloid malignancies in mice. Furthermore, downregulating the p50 subunit of NF-κB or IL-6 significantly dampened chronic inflammation-driven HSC exhaustion and oncogenesis, providing a potential strategy for intervention [62]. A strategy to target IRAK1, a kinase linking TLR and cytokine stimulation to NF-κB, has been tested in a preclinical model of MDS and shown to be effective in eliminating MDS cells while sparing normal human HSPCs [63]. One other area of clinical application lies in the utilization of cytokines to activate dormant leukemic stem cells for targeted molecular therapies. This has been tried in chronic myelogenous leukemia (CML) and polycythemia vera by combining interferon therapy with Bcr-Abl and JAK2 inhibitors, respectively [64,65]. In fact, combination of imatinib and IFNα was more effective in achieving a molecular response in (CML) patients in a phase III clinical trial; however, the mechanism underlying the improved efficacy was not studied [66,67]. Controlling chronic inflammation and targeting inflammatory signaling that is aberrantly activated in hematologic malignancies will be a promising chemopreventive and therapeutic strategy; furthermore, waking up leukemic stem cells from dormancy with PAMPs or cytokines may finally make them vulnerable to therapeutic targeting.

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Acknowledgements

None.

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Financial support and sponsorship

This work was supported by an R01 research grant from NIAID (D.B.) and a F30 NRSA fellowship from NHLBI (J.L.Z.) of National Institutes of Health.

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Conflicts of interest

There are no conflicts of interest.

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REFERENCES AND RECOMMENDED READING

Papers of particular interest, published within the annual period of review, have been highlighted as:

  • ▪ of special interest
  • ▪▪ of outstanding interest
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Keywords:

cytokines; hematopoiesis; hematopoietic stem cells; infection; inflammation; toll-like receptors

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