HEMATOPOIESIS: Edited by Hal E Broxmeyer and Maegan L CapitanoSplicing regulation in hematopoiesisChen, Sisia; Abdel-Wahab, Omara,bAuthor Information aHuman Oncology and Pathogenesis Program bLeukemia Service, Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, New York, USA Correspondence to Omar Abdel-Wahab, 1275 York Ave., New York, NY 10065, USA. Tel: +646 888 3487; e-mail: [email protected] Current Opinion in Hematology: July 2021 - Volume 28 - Issue 4 - p 277-283 doi: 10.1097/MOH.0000000000000661 Buy Metrics Abstract Purpose of review Splicing mutations are among the most recurrent genetic perturbations in hematological malignancies, highlighting an important impact of splicing regulation in hematopoietic development. However, compared to our understanding of splicing factor mutations in hematological malignancies, studies of splicing components and alternative splicing in normal hematopoiesis have been less well investigated. Here, we outline the most recent findings on splicing regulation in normal hematopoiesis and discuss the important questions in the field. Recent findings Recent studies have highlighted the critical role of splicing regulation in hematopoiesis, including characterization of splicing components in normal hematopoiesis, investigation of transcriptional alterations on splicing, and identification of stage-specific alternative splicing events during hematopoietic development. Summary These interesting findings provide insights on hematopoietic regulation at a co-transcriptional level. More high-throughput RNA ribonucleic acid (RNA) sequencing and functional genomic screens are needed to advance our knowledge of critical alternative splicing patterns in shaping hematopoiesis. Copyright © 2021 Wolters Kluwer Health, Inc. All rights reserved.