Secondary Logo

Journal Logo

Medical progress

Osteostimulation of bioglass

HU, Yong-cheng; ZHONG, Ji-pin

Author Information
doi: 10.3760/cma.j.issn.0366-6999.2009.19.035
  • Free

Bioglass, with the code name 45S5, contains 45% silica, 24.5% CaO, 24.5% Na2O and 6% P2O5 in weight percent. All of these oxides are mixed together and melted at around 1350°C to form a homogeneous silica network glass.1 Bioglass possesses fast biological response and fast bioactivity, when implanted in living tissue. Since Hench et al1 reported over 35 years ago that the bioglass composition could chemically bind to bone, the research and development of bioactive glasses has been a very active field, involving many research groups worldwide and tens of thousands of studies on bioactive glasses have been published. Still, so far, no other bioactive glass composition has been reported that demonstrates a faster biological response than bioglass.

Bioglass has been used as a synthetic bone graft material, with two products developed and used clinically for over 10 years in the US, Europe and China: NovaBone, a bone graft product used in the orthopedic field, and PerioGlas, a bone graft product used in dental and maxilofacial surgery. In 2005, the bioglass products were cleared by the US Food and Drug Administration (FDA) for osteostimulation: “The stimulation of osteoblast proliferation and differentiation as evidenced during in vitro osteoblast cell culture studies by increased DNA content and elevated osteocalcin and alkaline phosphatase levels”. It has been over 35 years from the first report of chemical bonding to bone to the now recognized bone stimulation function of bioglass.

For a bone graft material, osteoinduction, osteostimulation and osteoconduction are 3 levels of biological response with osteoinduction being the highest level. The osteoinductive graft materials such as bone morphogenetic protein (BMP) and autogenous bone are able to induce stem cell to bone cell transition and are supposed to promote new bone growth in the bony defect area at a fast rate. Osteostimulation is an intermediate level. The osteostimulative graft material can enhance the production of growth factors and promote the proliferation and differentiation of bone cells, which stimulate new bone formation and growth, and new bone can be seen simultaneously at the edge and center of the defect area. Osteoconductive graft materials can serve as a scaffold and the native bone grows along their surface from the edge of the defect to the center. A number of bioceramics such as calcium phosphate and calcium sulfate belong to this group. The accumulated evidence demonstrates that bioglass is both osteoconductive and osteostimulative.

This article reviews more than 35 years of research from in vitro, cell culture to animal studies of bioglass and the present evidence indicating its osteostimulative property.


In their first published article in 1971, Hench et al1 reported that the bioglass composition could chemically bond to bone and its fast surface reaction was attributed to the bonding ability. The surface reaction takes place immediately upon bioglass contacting a physiological environment such as simulated body fluid (SBF)2 or body fluid. The surface reaction includes the ion exchange between Na+ from bioglass and H+ from the fluid, and the formation of a porous silica rich layer on the surface, then, followed by the formation of hydroxyl-carbonate-apatite (HCA), a similar mineral phase of bone, on the bioglass surface.3-11

For over 20 years from 1971, the research focused mainly on the correlation of the surface reaction of bioglass with bone bonding, and it was found that the formation of the HCA layer was essential for bone bonding. If no HCA formed on the bioactive glass compositions there would be no bone bonding for those compositions either.3,8 Bioglass showed fast HCA formation and also demonstrated fast and strong bone bonding.8 In one study,12 bioglass disks were reacted in Tris buffer solution, one kind of physiological solution, with type I collagen fibers up to 7 days. Figure 1 is the photograph of scanning electron microscopy (SEM) of the bioglass surface after day 1 and day 7 in the solution. Clearly, the collagen fibers attached to the bioglass surface and the HCA formed on the surface embedding the collagen fibers. This demonstrated the bonding process of the bioglass surface to bone, and also demonstrated a similar process of bone formation. The discovery was very encouraging because the process occured without the existence of bone cells. The result for the bone bonding and bone formation could be better if bioglass was placed under living conditions, and it was assumed that bioglass could have great osteoconductive ability.

Figure 1.
Figure 1.:
SEM of bioglass surface after reacting in Tris/collagen fibers solution (From the file of and permitted by NovaBone Products, LLC, USA. Original magnification). A: Day 1. B: Day 7.

In 2002, a study examined13 the microstructure of bone-like apatite formed on the bioglass surface and also the bone-like apatite that was found in the physiological solution, SBF, when reacted with bioglass particles. This study again demonstrated the potential promotion of the mineralization process of bone formation by bioglass.


Cell proliferation

Many articles have been published on bioglass cell culture.14-17 Osteoblast proliferation and high activity of DNA synthesis were always reported in those articles. In 1993, Vrouwenvelder et al16 reported that when bioglass was cultured with osteoblasts from 20-day-old fetal rats, it showed a better osteoblast character and higher mean proliferation rate than other materials such as hydroxylapatite (HA), titanium alloy and stainless steel. The alkaline phosphatase (ALP) activity was 3 times higher and DNA content was 35% more for bioglass cultures in 8 days compared to HA. Price et al17 reported a higher proliferation rate and higher concentration of osteocalcin (OC) when osteoblasts were cultured with bioglass. All of the results, the higher ALP and OC production and higher DNA content, showed higher bone formation activity of osteoblasts when cultured with bioglass.

Actual bone formation has been observed by SEM on a bioglass surface when cultured with osteoblasts. After 22 days of culture, SEM examination revealed the mineralized bone attached to the bioglass surface (Figure 2) and also, it can be clearly seen that bone with nodular structure bonds well with bioglass (Figure 3).

Figure 2.
Figure 2.:
Mineralized bone attached on the bioglass surface after 22 days osteoblast culture (Courtesy of Dr. JM Sautier, Laboratoire de Biologie, Univ Paris, France)
Figure 3.
Figure 3.:
Bone nodular formed on bioglass surface after 22 days osteoblast culture (Courtesy of Dr. JM Sautier, Laboratoire de Biologie, Univ Paris, France)

Activation of gene expression

In 2000, Xynos et al18 reported that the ionic products from bioglass degradation or dissolution not only increased the proliferation of human osteoblasts but also induced insulin-like growth factor II mRNA expression and protein synthesis. In his series of studies, Xynos et al19 also reported the effect of the ionic products of bioglass 45S5 dissolution on the gene-expression profile of human osteoblasts by cDNA microarray analysis of 1176 genes. A total of 190 out of the 1176 genes surveyed in this study were shown to be expressed in human osteoblasts at levels above background. The expression of the genes from his works is partially listed in Table. The activation of osteoblast gene expression by bioglass from this study provided direct evidence to support the osteostimulation of bioglass at the molecular level. The activation of genes such as cell surface receptors, signal transduction molecules, growth factors, cell cycle regulators, matrix component, DNA synthesis etc, would accelerate the proliferation or growth of bone cells, hence, stimulate new bone formation and growth.

Genes expression in human osteoblasts treated with the ionic products of bioglass dissolution 20

Change of cell growth cycle

As has been discussed above, bioglass can enhance the proliferation and differentiation of osteoblasts and produce a high content ALP and OC. Xynos et al20 also reported in 2000 that the cell populations in both S phase (DNA synthesis) and G2/M phase (mitosis) of the cell cycle were increased when osteoblasts were cultured with bioglass. As a continuous work, Sun et al21 reported the same result and in his quantitative analysis the populations of the osteoblasts in both S phase and G2/M phase that were cultured with a bioglass solution reached a maximum in 2 days. In their study, human osteoblasts were isolated from trabecular bone of femoral heads from total hip arthroplasty, and the second passage of the osteoblasts was used. Compared with the osteoblasts cultured with just culture medium, in which, the S phase and G2/M phase reached a maximum in 4 days (Figure 4), bioglass solution achieved maximum cell division 2 days early as a result of a shorter cell growth cycle, and faster cell turnover. This result is coincident with the activation of cell cycle regulators by bioglass as discussed above. The short cell growth cycle and fast cell turnover again provide direct evidence for the osteostimulation of bioglass.

Figure 4.
Figure 4.:
The distribution of osteoblasts in S phase (A) and G2/M phase (B)


The osteostimulation of bioglass, observed in cell culture studies in vitro, can be directly reflected by fast and early bone formation in animal models. Although there have been several animal models, a typical model used for bioglass studies is the distal femur defect model in rabbits (Figure 5). In 1997, Oonishi et al22 reported that, beginning at one week, bioglass generated new bone faster than HA; and in 6 weeks, new bone formed in the entire defect area with 100% new bone penetration into the defect by bioglass (Figure 6), while there was only 30% new bone penetration into the defect by HA.

Figure 5.
Figure 5.:
Transfemoral defect, 6 mm in diameter, in rabbit distal condoyle.
Figure 6.
Figure 6.:
Percentage of new bone penetration into defect: bioglass vs HA.

Chou et al23 reported their study on the rabbit model using bioglass that new bone formation activity was found within 2 weeks of bioglass implantation, at this early stage of implantation bioglass stimulated osteogenic cells at the interface to generate a significantly higher level of osteocalcin expression, resulting in a remarkably increased bone formation.

In 2000, Wheeler et al24 reported her rabbit study using bioglass and the results can be seen in the histological pictures in Figure 7. In Figure 7 B, the red color zone represents bone while G represents bioglass particles. Bioglass particles are distributed in the defect by implantation (Figure 7 A), and in 4 weeks (Figure 7 B), new bone (B) formed around the graft particles (G). In 12 weeks (Figure 7 C), significant new bone formed around the graft particles and in the whole defect area.

Figure 7.
Figure 7.:
New bone formation stimulated by bioglass in rabbit model (Courtesy of Dr. D.L. Wheeler, Department of Orthopaedics, University of Florida, Gainesville, USA).

As a recent and future direction, the research with bioglass has already turned from direct osteoblast and bone tissue growth to its resorption,25 antimicrobial,26 antibacterial,27 and anti-inflammatory,28 potential and its application for tissue engineering.29 Reports of more of this work can be expected in publications in the coming years.


1. Hench LL, Splinter RJ, Allen WC, Greenlee TK. Bonding mechanisms at the interface of ceramic prosthetic materials. J Biomed Mater Res 1971; 5: 117-141.
2. Kokubo T, Kushitani H, Sakka S, Kitsugi T, Yamamuro T. Solution able to reproduce in vivo surface-structure changes in bioactive glass-ceramic A-W. J Biomed Mater Res 1990; 24: 721-734.
3. Hench LL, Paschall HA. Direct chemical bond of bioactive glass-ceramic materials to bone and muscle. J Biomed Mater Res 1973; 7: 25-42.
4. Pantano CG Jr, Clark AE Jr, Hench LL. Multi-layer corrosion films on Bioglass. J Am Ceram Soc 1974; 57: 412-413.
5. Clark AE, Pantano CG, Hench LL. Auger spectroscopic analysis of bioglass corrosion films. J Am Ceram Soc 1976; 59: 37-39.
6. Ogino M, Ohuchi F, Hench LL. Compositional dependence of the formation of calcium phosphate films on bioglass. J Biomed Mater Res 1980; 14: 55-64.
7. Kim CY, Clark AE, Hench LL. Early stages of calcium-phosphate layer formation on bioglasses. J Non Crystal Solids 1988; 113: 195-202.
8. Hench LL. Bioceramics: From concept to clinic. J Am Ceram Soc 1991; 74: 1487.
9. Filgueiras MR, La Torre G, Hench LL. Solution effects on the surface reactions of a bioactive glass. J Biomed Mater Res 1993; 27: 445-453.
10. Hench LL, West JK. Biological applications of bioactive glasses. Life Chemistry Reports 1996; 13: 187-241.
11. Zhong JP, Greenspan DC. Bioglass surface reactivity: from in vitro to in vivo. Bioceramics 1998; 11: 415-418.
12. Internal Research Report, In file, NovaBone Products, LLC.
13. Zhong JP, Greenspan DC, Feng JW. A microstructural examination of apatite induced by Bioglass in vitro. J Mater Sci Mater Med 2002; 13: 321-326.
14. Matsuda T, Davies JE. The in vitro response of osteoblasts to bioactive glass. Biomaterials 1987; 8: 275-284.
15. Vrouwenvelder WC, Groot CG, de Groot K. Behaviour of fetal rat osteoblasts cultured in vitro on bioactive glass and nonreactive glasses. Biomaterials 1992; 13: 382-392.
16. Vrouwenvelder WC, Groot CG, de Groot K. Histological and biochemical evaluation of osteoblasts cultured on bioactive glass, hydroxylapatite, titanium alloy, and stainless steel. J Biomed Mater Res 1993; 27: 465-475.
17. Price N, Bendall SP, Frondoza C, Jinnah RH, Hungerford DS. Human osteoblast-like cells (MG63) proliferate on a bioactive glass surface. J Biomed Mater Res 1997; 37: 394-400.
18. Xynos ID, Edgar AJ, Buttery LD, Hench LL, Polak JM. Ionic products of bioactive glass dissolution increase proliferation of human osteoblasts and induce insulin-like growth factor II mRNA expression and protein synthesis. Biochem Biophys Res Commun. 2000; 276: 461-465.
19. Xynos ID, Edgar AJ, Buttery LD, Hench LL, Polak JM. Gene-expression profiling of human osteoblasts following treatment with the ionic products of Bioglass 45S5 dissolution. J Biomed Mater Res 2000; 55: 151-157.
20. Xynos ID, Hukkanen MV, Batten JJ, Buttery LD, Hench LL, Polak JM. Bioglass 45S5 stimulates osteoblast turnover and enhances bone formation in vitro: implications and applications for bone tissue engineering. Calcif Tissue Int 2000; 67: 321-329.
21. Sun JY, Yang YS, Zhong J, Greenspan DC. The effect of the ionic products of Bioglass dissolution on human osteoblasts growth cycle in vitro. J Tissue Eng Regen Med 2007; 1: 281-286.
22. Oonishi H, Kushitani S, Yasukawa E, Iwaki H, Hench LL, Wilson J, et al. Particulate bioglass compared with hydroxyapatite as a bone graft substitute. Clin Orthop Relat Res 1997; (334): 316-325.
23. Chou L, Al-Bazie S, Cottrell D. Atomic and molecular mechanisms underlying the osteogenic effects of bioglass materials. Bioceramics 1998; 11: 265.
24. Wheeler DL, Eschbach EJ, Hoellrich RG, Montfort MJ, Chamberland DL. Assessment of resorbable bioactive material for grafting of critical-size cancellous defects. J Orthop Res 2000; 18: 140-148.
25. Gaisser DM, Wheeler DL, Greenspan DC. In vivo absorbability of 45S5 Bioglass® bioactive glass - A histologic analysis. Trans. 28th Annual Meeting Society for Biomaterials. 2002; 25: 400.
26. Gaisser DM, Greenspan DC. Antimicrobial properties of 45S5 bioactive glass particulate. Presented at the Society for Biomaterials 30th Annual Meeting and Exposition, Memphis, TN, Transaction. 2005: 558.
27. Allan I, Newman H, Wilson M. Antibacterial activity of particulate bioglass against supra- and subgingival bacteria. Biomaterials 2001; 22: 1683-1687.
28. Rectenwald JE, Minter RM, Rosenberg JJ, Gaines GC, Lee S, Moldawer LL. Bioglass attenuates a proinflammatory response in mouse peritoneal endotoxicosis. Shock 2002; 17: 135-138.
29. Hench LL, Xynos ID, Polak JM. Bioactive glasses for in situ tissue regeneration. J Biomater Sci Polym Ed 2004; 15: 543-562.

bioglass; osteogenesis; bone substitutes

© 2009 Chinese Medical Association