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Catechol-O-Methyltransferase (COMT) Gene Polymorphisms as Risk Factor in Temporomandibular Disorders Patients From Southern Italy

Michelotti, Ambrosina DDS*; Liguori, Rosario MSc; Toriello, Mario MSc; D’Antò, Vincenzo DDS, PhD*; Vitale, Dino MD; Castaldo, Giuseppe MD, PhD; Sacchetti, Lucia MSc, PhD

doi: 10.1097/AJP.0b013e318287a358
Original Articles

Objectives: To evaluate the role of COMT gene variants as potential risk factors in a group of patients affected with chronic temporomandibular disorder (TMD) pain.

Methods: We sequenced COMT gene in 182 Italian subjects (50 affected by TMD and 132 controls). The study population consisted of patients affected by myogenous and/or arthrogenous pain (RDC/TMD: Ia, Ib, IIIa, IIIb diagnostic categories).

Results: We detected 40 single nucleotide polymorphisms (SNPs) variants (18 novel). Three SNPs, all located in the promoter regions, were more frequently present in cases than in controls (rs 4646310 P=0.018, rs165656 P=0.001, rs 165722 P=0.007). After the False Discovery Rate (FDR) correction rs165656 remained significantly associated with TMD (P=0.049). In addition, the rs 4646310 (AG vs GG, P=0.015) and rs 165656 (GG vs CC, P=0.001) were at binary logistic regression analysis independently associated with TMD, conferring a risk disease of 2.6 (CI= 1.2-5.6) and of 5.3 (CI= 2.0-13.7) respectively.

Discussion: Our data extend the number of SNPs present in the promoter region that could play a regulatory role in COMT gene and suggest that the genetic polymorphisms rs 165656 and rs 4646310 exert a role in TMD susceptibility.

Supplemental Digital Content is available in the text.

*Department of Oral and Maxillo-Facial Sciences, Section of Orthodontics and Clinical Gnathology

CEINGE Biotecnologie Avanzate S.C.a R.L., Department of Biochemistry and Medical Biotechnology, University of Naples “Federico II,” Naples

Salvatore Maugeri Foundation, IRCCS, Benevento, Campania, Italy

Supplemental Digital Content is available for this article. Direct URL citations appear in the printed text and are provided in the HTML and PDF versions of this article on the journal's Website,

A.M. and R.L. contributed equally.

The authors declare no conflict of interest. Supported by Grants from Ceinge-Regione Campania, DGRC 1901/2009, and MIUR P.S. 35-126/IND, Napoli, Italy.

Reprints: Ambrosina Michelotti, DDS, University of Naples “Federico II,” Via Pansini 5-0131, Naples, Italy (e-mail:

Received June 6, 2011

Accepted January 12, 2013

© 2014 by Lippincott Williams & Wilkins