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Increased Capsaicin Receptor TRPV1 in the Peritoneum of Women With Chronic Pelvic Pain

Poli-Neto, Omero Benedicto MD* †; Filho, Antonio Adnor MS; Rosa e Silva, Júlio César MD§; Barbosa, Hermes de Freitas MD§; Candido Dos Reis, Francisco José MD; Nogueira, Antonio Alberto MD

doi: 10.1097/AJP.0b013e318188378a
Original Articles

Objective To investigate the expression of capsaicin receptor [transient receptor potential vanilloid type-1 (TRPV1)] in the peritoneum of women with chronic pelvic pain (CPP).

Methods A case-control study was conducted on 25 women with CPP and 10 controls. Samples of the rectouterine excavation (2 cm2) were obtained by laparoscopy, fixed in 4% formaldehyde, and underwent immunohistochemistry analysis using rabbit anti-TRPV1 (1:400) polyclonal antibodies and anti-protein gene product 9.5 (PGP 9.5) (1:2000) as a neuronal marker. Ten sequential images of high magnification fields (×40) were captured from each slide and the area identified with the antibody was calculated with Kontron V2.0 software.

Results Immunoreactivity to TRPV1 was sparsely detected in the nervous tissue and epithelium of endometriotic lesions. The percent area of immunoreactivity for TRPV1 [expressed as median (range)] was greater in specimens from women with CPP, 1.02% (0.54 to 2.93), than from women without the disease, 0.14% (0.07 to 1.12) (P<0.0001). This greater expression was not secondary to an increase in neuronal fibers because there was also a significant difference in the percent area TRPV1:PGP 9.5 ratio between women with CPP, 1.18 (0.26 to 4.63), and controls, 0.15 (0.06 to 0.95) (P=0.0003).

Discussion TRPV1 may play an important role in the maintenance and perpetuation of symptoms in women with CPP. In view of the immunoreactivity detected for TRPV1, the endometriotic lesion may have the ability to interfere with nociception or with the inflammatory peritoneal environment in women with CPP. Further studies are needed to elucidate the participation of TRPV1 in CPP and its association with endometriosis.

Departments of *Surgery and Anatomy

Gynecology and Obstetrics

Faculty of Medicine of Ribeirão Preto

§University Hospital, University of São Paulo, SP, Brazil

Reprints: Omero Benedicto Poli-Neto, MD, Avenida Bandeirantes, 3900, Campus Universitário, Monte Alegre, 14049-900 Ribeirão Preto, SP, Brazil (e-mail:

Received for publication April 14, 2008; revised July 24, 2008; accepted July 28, 2008

© 2009 Lippincott Williams & Wilkins, Inc.