Secondary Logo

Journal Logo

Institutional members access full text with Ovid®

Oxidative Stress and Genotoxicity in Pterygium

A Systemic Investigation

Kilic-Toprak, Emine Ph.D.; Toprak, Ibrahim M.D.; Caliskan, Sadettin Ph.D.; Ozdemir, Yasin M.D.; Demirtas, Onder M.D.; Altintas, Fatih M.D.; Kucukatay, Vural Ph.D.

doi: 10.1097/ICL.0000000000000620

Objectives: To perform a systemic investigation on oxidative stress and DNA damage in patients with primary pterygium.

Methods: This prospective cross-sectional study included 32 patients with primary pterygium (60.1±2.0 years of age) and 33 age- and sex-matched (58.8±2.2 years of age) control subjects (P>0.05). A commercial kit was used for measuring serum total oxidant status (TOS) and total antioxidant status (TAS). The comet assay was performed after lymphocyte isolation from venous blood to quantitate DNA damage. Tail length (TL), tail intensity (TI), and tail moment (TM) were used for statistical analysis as parameters of DNA damage.

Results: In the pterygium group, TOS and TAS were significantly higher when compared with those of the control group (P=0.019 and P=0.005, respectively). In terms of DNA damage, patients with pterygium had higher TL, TI, and TM than in the control subjects (P<0.0001 for all).

Conclusions: Although current literature focuses on local factors in pterygium pathogenesis, patients with pterygium seem to have increased systemic oxidative status (and compensatory antioxidant response) and genotoxicity, which might create a predisposition for pterygium development.

Departments of Physiology (E.K.-T, S.C., Y.O., F.A., V.K.), and Ophthalmology (I.T., O.D.), Faculty of Medicine, Pamukkale University, Denizli, Turkey.

Address correspondence to Ibrahim Toprak, M.D., Department of Ophthalmology, Faculty of Medicine, Pamukkale University, Kinikli 20125, Denizli, Turkey; e-mail:

The authors have no funding or conflicts of interest to disclose.

Supported by Pamukkale University Scientific Research Projects Coordination Unit (Project number: 2018HZDP048).

The preliminary data from this study were presented at the 44th National Congress of Turkish Society of Physiological Sciences as a poster presentation, November 01–04, 2018, Antalya, Turkey.

Accepted March 31, 2019

© 2019 Contact Lens Association of Ophthalmologists, Inc.