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Miraflow, Soft Contact Lens Cleaner: Activity Against Acanthamoeba Spp

Kobayashi, Takeshi Ph.D.; Higuchi-Watanabe, Narumi M.S.; Shiraishi, Atsushi M.D., Ph.D.; Uno, Toshihiko M.D., Ph.D.; Ohashi, Yuichi M.D., Ph.D.

Eye & Contact Lens: Science & Clinical Practice: July 2015 - Volume 41 - Issue 4 - p 240–244
doi: 10.1097/ICL.0000000000000112

Objective: Miraflow is a cleaner for soft contact lens which contains 20% isopropyl alcohol. The purpose of this study was to determine the activity against Acanthamoeba trophozoites and cysts for Miraflow. In addition, to determine the activity of combined Miraflow and multipurpose solutions (MPSs) against Acanthamoeba cysts.

Methods: Two simulated-use studies were conducted. The significance in the log reduction in the number of trophozoites and cysts of A. castellanii strains ATCC 50514 and ATCC 50370 or A. polyphaga ATCC 30461 after exposure to Miraflow alone was determined by the Spearman–Karber method. To examine the activity against Acanthamoeba of combined Miraflow and an MPS, the log reduction in the number of cysts after a 1-min exposure to Miraflow followed by a 4-hr exposure to MPS (ReNu fresh) was also determined.

Results: Short-time exposure of 30 sec to Miraflow demonstrated activity against the Acanthamoeba trophozoites. However, a 1-min treatment was only relatively effective (1.1 log reduction) against the cysts of A. castellanii ATCC 50514, but no statistically significant reduction was observed for the cysts of the other 2 strains. The combined use with Miraflow and MPS demonstrated activity against the cysts, and a 3.0, 1.0, or 1.5 log reduction in the numbers was obtained for A. castellanii ATCC 50514, A. castellanii ATCC 50370, and A. polyphaga ATCC 30461, respectively.

Conclusions: Exposure to combined Miraflow and MPS resulted in reductions in the number of Acanthamoeba cysts.

Departments of Ophthalmology and Regenerative Medicine (T.K.), and Ophthalmology (N.H.-W., A.S., T.U., Y.O.), Ehime University School of Medicine, Toon, Japan.

Address correspondence to Takeshi Kobayashi, Ph.D., Department of Ophthalmology and Regenerative Medicine, Ehime University Graduate School of Medicine, Shitsukawa, Toon, Ehime 791-0295, Japan; e-mail:

The Department of Ophthalmology, Ehime University School of Medicine, has received research funding from CIBA Vision (Tokyo, Japan).

T. Kobayashi has received an honorarium from CIBA Vision for writing this article. The other authors have no conflicts of interest to disclose.

Accepted September 30, 2014

© 2015 Contact Lens Association of Ophthalmologists, Inc.