We sought to determine whether there were differences in lysozyme (quantity and conformation) and lipid deposition on in vivo worn conventional (etafilcon) and silicone hydrogel (balafilcon and lotrafilcon) contact lenses.
After extraction, lysozyme concentration in each extract was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. Lysozyme activity was determined by the rate of lysis of Micrococcus lysodeikticus cells. Lipid deposition was determined by high-performance liquid chromatography.
Lysozyme deposition on etafilcon lenses was significantly greater than that measured on silicone hydrogel (SH) lenses (985 μg per lens versus 10 and 3 μg per lens for balafilcon and lotrafilcon materials, respectively;P <0.001). The degree to which lysozyme was denatured was influenced by the lens material, with the lowest degree of denaturation (22%) seen on the conventional lens material, as compared with 50% for balafilcon and 80% for lotrafilcon (P <0.001). Lipid deposition was greatest on the SH materials, with up to 600 μg per lens of certain lipid classes being deposited on balafilcon, as compared with 20 μg per lens on etafilcon (P <0.001).
The quantity and conformation of lysozyme and the quantity of lipid deposited on hydrogel contact lenses is significantly influenced by the composition of the lens material. SH contact lens materials deposit low levels of lysozyme and high levels of lipid deposition compared with ionic contact lens materials. Although SH materials deposit only small amounts of lysozyme, the degree of lysozyme denaturation that occurs is higher relative to that seen on ionic lens materials.
Centre for Contact Lens Research, School of Optometry, University of Waterloo, Waterloo, Canada.
Accepted October 8, 2002.
Address correspondence to: Lyndon Jones, Ph.D., Centre for Contact Lens Research, School of Optometry, University of Waterloo, 200 University Avenue, Waterloo, Canada, N2L 3G1. Phone: (519) 888-4567 ext 5030; fax: (519) 884-8769; e-mail: email@example.com.
Supported in part by the National Sciences and Engineering Research Council of Canada and the Canada Foundation for Innovation.