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Evaluation of Contamination of Used Disposable Contact Lenses by Acanthamoeba

Sadiq S Ahmed MBBS DO MRCOphth FRCS; Azuara-BIanco, Augusto MD, PhD; Bennett, D MCOptom; Lloyd, John H. BSc, PhD; Dua, Harminder S. MD, PhD, FRCS.
Eye & Contact Lens: July 1998
Contact Lenses: PDF Only


The purpose of this study was to evaluate the contamination of used disposable soft contact lenses with Acanthamoeba.


We evaluated 51 consecutive disposable soft contact lens wearers in a prospective study. Before their check-up visits, patients were offered a free new contact lens in exchange for donating their old one. The used contact lenses (n=102) were then removed and placed in culture medium for Acanthamoeba. We subsequently investigated patterns of wear and hygiene habits.


The mean age of this population was 32.6±11.0 years. The average time of disposable soft contact lens wear was 13.7 hours per day. The contact lenses were disinfected daily with commercial solutions. Twenty-five (49.0%) subjects did not clean their lens cases properly. Acanthamoeba was not isolated from any of the 102 lenses.


There were no cases of Acanthamoeba contamination in a small population of disposable soft contact lenswearers who regularly disinfected their lenses with standard commercially available contact lens solutions.

© 1998 The Contact Lens Association of Ophthalmologists, Inc.