Secondary Logo

Share this article on:

Australasian College of Sports Physicians—Position Statement: The Place of Mesenchymal Stem/Stromal Cell Therapies in Sport and Exercise Medicine

Osborne, Hamish MMedSci; Anderson, Lynley PhD; Burt, Peter MHealSci; Young, Mark MBBS; Gerrard, David MBChB

Clinical Journal of Sport Medicine: March 2016 - Volume 26 - Issue 2 - p 87–95
doi: 10.1097/JSM.0000000000000298
Position Statement
Podcast

*Department of Medicine, Dunedin School of Medicine, University of Otago, Dunedin, New Zealand;

Bioethics Centre, University of Otago, Dunedin, New Zealand; and

Mater Medical Research University of Queensland and Queensland Institute of Technology, Brisbane, Australia.

Corresponding Author: Hamish Osborne, MMedSci, Dunedin School of Medicine, University of Otago, PO Box 56, Dunedin, New Zealand (hamish.osborne@otago.ac.nz).

The authors report no conflicts of interest.

The Australasian College of Sports Physicians has granted the Publisher permission for the reproduction of this article.

Received November 10, 2015

Accepted December 05, 2015

Back to Top | Article Outline

PURPOSE AND SCOPE

This position statement has been written expressly for members of the Australasian College of Sports Physicians (ACSP); however, it may also be of interest to the wider medical community, sporting organizations, athletes, and the general community.

It has been informed by a comprehensive review of the scientific literature and the opinions of kindred organizations.

This statement outlines the use of mesenchymal stem cell (MSC) therapies in the broad context of Sport and Exercise Medicine, recognizing that every medical practitioner should respect:

  1. The evidence for the therapeutic use of MSCs.
  2. The priority for patient health and welfare.
Back to Top | Article Outline

INTRODUCTION/BACKGROUND

Mesenchymal Stem Cells

Although many types of stem cell exist, they all possess 2 distinct properties. First, the ability to self-replicate (proliferate), and second, their potency or ability to differentiate (transform into mature adult tissue). Stem cells derived from and returned to the donor are termed “autologous,” whereas those administered to a non–tissue-matched recipient are called as “allogeneic” stem cells.

Mesenchymal stem cells are derived from stromal tissue and described as multipotent, having the capacity to differentiate into mesodermal and endodermal types of cells, depending on the tissue matrix.1 Their multipotency, specific surface antigen expression, and their plastic-adherence properties when cultured are required for the definition of MSCs.2

This position statement only applies to MSC therapies and not to embryonic, hematopoietic, or induced pluripotent stem cells; cellular therapies involving autologous tenocytes, autologous chondrocytes, or platelet therapies.

Back to Top | Article Outline

Expanding Mesenchymal Stem Cell Populations

Mesenchymal stem cells can be extracted from adipose and synovial tissue, peripheral blood, skeletal muscle, umbilical cord blood, placenta, and bone marrow. Generating high numbers of MSCs requires several steps. First, harvesting, commonly from bone marrow or adipose tissue, then a stage of isolation and finally encouragement to proliferate. This MSC expansion process takes several weeks, is costly, highly technical but devoid of a “gold standard.” The resulting manufactured cells are called mesenchymal stromal cells or mesenchymal progenitor cells. The MSC expansion processes result in variable cell populations with different cellular properties.3 The expanded cell populations also display reduced migration differentiation with consequences on their capacity for graft formation.4–8 The lack of standardization between the commercially available MSC expansion protocols makes comparisons between products and studies relating to those products difficult.9

A contemporary development in manufacturing techniques uses far shorter preparation times (often less than 2 hours) at far less cost, producing a nonexpanded mixed cell population. However, MSC numbers are low in these preparations and the presence of multiple cell types raises questions about the efficacy of these techniques.10 Although the progress in biotechnology has resulted in commercially attractive outcomes, the science substantiating their effectiveness has lagged behind. This has been called an “unhealthy system.”11 No published randomized controlled trials for “same-day bone marrow stem cell concentrates” are available as of 2015.10,12

Back to Top | Article Outline

Mesenchymal Stem Cells and Their Therapeutic Utility

Mesenchymal stem cells secrete growth factors and cytokines with trophic, chemotactic, and immunosuppressive properties.2,7,8,13,14 Experimental studies in animals show their potential for influencing musculoskeletal tissue regeneration and enhancement rather than fundamental repair, as would occur in simple scar formation.15–17 Therefore, MSCs have a potential regenerative influence on tissues damaged by repetitive overuse or through age-related degeneration. The preprogramming of MSCs to form a specific tissue has intriguing theoretical consequences for tissue regeneration and potential enhancement.

Given their low numbers, MSC populations for therapeutic use need to be expanded as outlined earlier. The ideal prerequisites for a satisfactory graft are large numbers of viable MSCs that do not age prematurely, and with the capacity to differentiate accurately into the correct cell type of the target site. Some consider that before clinical trials proceed it is important to determine that the success of the graft as the consequence of MSC activity rather than the paracrine effects of the grafted material.18 There are still many unresolved challenges to the therapeutic use of MSC's.19

Back to Top | Article Outline

Clinical Trials and Mesenchymal Stem Cell Therapies

There is no evidence in the contemporary literature of any MSC therapy for use in musculoskeletal conditions having successfully completed a full clinical trial in accordance with the steps mandated by the International Society for Stem Cell Research.20 This protocol strictly adheres to a 4-phase model of clinical trial necessary for any novel therapeutic intervention. Consequently, current variants of MSC therapy advanced by clinicians throughout Australasia have limited scientific credibility, may not meet the requirements for evidence-based clinical practice nor satisfy the criteria for patient safety.21

Back to Top | Article Outline

The Fundamental Steps of the Widely Accepted Clinical Trial Process Include

Phase I

Researchers test the safety and assess adverse effects of an intervention in a small cohort of participants.22,23

Back to Top | Article Outline

Phase II

The intervention is administered to a larger group to assess efficacy and to further evaluate safety.

Back to Top | Article Outline

Phase III

The treatment is administered to large identified groups of participants to confirm effectiveness, monitor side effects, provide comparisons with commonly used treatments, and to collect information that will inform future safe treatment.

Back to Top | Article Outline

Phase IV

Post market surveillance is undertaken (after the introduction of the therapy to clinical practice) to assess any adverse effects associated with long-term use.

There have been a number of concerns raised about the use of MSCs in clinical practice in the absence of evidence of clinical efficacy. This article will address the scientific and ethical concerns.

Back to Top | Article Outline

SAFETY

Mesenchymal stem cells by definition have the ability to transform/differentiate into a limited number of mature cell types (multipotency), making them different from embryonic stem cells that have pluripotent differentiation potential. This makes MSCs a safer option. However, questions remain including:

  1. Do MSCs have a tumorigenic effect?
  2. What is the mechanism of action of the therapeutic effect?
  3. Is greater therapeutic effect associated with greater risk?
  4. Do MSCs stimulate currently dormant tumor cells within the recipient?

Before phase I research can commence, often preclinical animal models are used to better understand safety and adverse effects of innovative biological therapies. Suitable models for comparison to humans may be available,24 but sometimes artificial biological models need to be developed to mimic human disease state to assess biological response.24

Safety studies need to be rigorous in design, including strong institutional framework of peer review in the development phase, stringent ethical approval, implementation and follow-up to predetermined measurable and clinically relevant endpoints.25

A systematic review and meta-analysis (2012) of the intravenous use of MSCs14 in 321 patients with a diverse group of medical conditions, including Crohn Disease, stroke, myocardial infarction, and cardiomyopathy, found their use to be safe. However, there have been 3 subsequent case reports of similar interventions that describe significant side effects–ventricular fibrillation, pulmonary emboli, and acute disseminated encephalomyelitis.26–28

Malignancy associated with pluripotent stem cells in preclinical studies is of concern. In the case of MSCs, quality evidence of the risk of malignant transformation in humans is lacking.13,14 There is a case report of transplanted autologous cells, presumed to be MSCs, causing a tumor 8 years later.29 The literature continues to question the potential for stem cell use to unmask dormant malignancy.13,30 A study of bone marrow-derived stem cells cultured for 4 weeks showed 2 of 5 cultures formed sarcoma-like tumors in vitro.31 A 4-week culture time is longer than MSCs currently available from commercial techniques; however, their tumor-forming potential remains a concern. If research suggests that larger numbers of stem cells are required for efficacy, then techniques of in vitro culture deserve further investigation. Only then can their in vivo application be considered to have no potential for tumorogenesis.

To date, research into the safe use of MSCs has not demonstrated tumor-forming potential or other significant side effects in phase I and II musculoskeletal research.32–34 However long-term safety cannot be assured. By way of example, agents later withdrawn for reasons of safety include the COX-2 Inhibitors Rofecoxib35,36 (Vioxx; Merck, Kenilworth) and Lumiracoxib37 (Prexige; Novartis, Basel, Switzerland). Both were withdrawn from the Australasian market several years after their introduction because of significant morbidity and mortality not evident in phase I and II research38 but discovered through vigilant postmarketing research (phase IV) after thousands of patients had been treated.

Back to Top | Article Outline

Safety in Osteoarthritis

A systematic review32 of the safety of autologous bone marrow derived MSCs in the treatment of osteoarthritis reviewed 3039 articles and found 8 articles that met their inclusion criteria.39–46 Eight hundred forty-four procedures were followed for a mean of 21 months. Increased pain and swelling after the injection were the only adverse events reported as being related to the stem cell product. Importantly the authors concluded that it is safe to continue development of stem cell therapies, provided there is “continuous caution for potential side effects.”32

A phase I/II double-blind, randomized, controlled trial of 59 patients injected post arthroscopic meniscal surgery showed no difference between minor and major side effects between the low dose (50 × 106 cells), high dose (150 × 106 cells), and control groups and no ectopic tissue formation. The MSCs were from bone marrow aspirates of unrelated donors (allograft).47

A further study of 91 patients in whom 100 joints were injected with autologous adipose MSCs were followed for an average of 26 months. No tumors were reported.48 However, swelling of the injected joints was reported as a common, but self-limiting side effect.

Several small studies have used MSCs as an adjunct to surgical intervention for the treatment of osteochondral defects in the ankle and knee.49–54 A total of 101 patients received MSCs compared with 51 control patients who had surgery without MSCs. There were no reported differences in significant adverse effects between groups.

Three, small phase I/II studies comparing high tibial osteotomy (n = 66) with high tibial osteotomy plus MSCs (n = 74) showed no significant side effects between groups after a 2-year follow-up.55–57 There are other studies involving orthopedic intervention, where MSCs were used as an adjunct, without any reported significant side effects.42,58–62

Back to Top | Article Outline

Safety in Tendinopathy

Few studies have examined the treatment of tendinopathy in humans with MSCs.24,30,33,34,63

Two case series treating patellar tendinopathy33 and 30 patients with lateral epicondylosis34 with bone marrow-derived nonexpanded MSCs followed for 5 years and 12 weeks, respectively, reported no significant adverse effects. Another small series of 14 patients who had bone marrow-derived nonexpanded MSCs injected around the surgical repair margins of full-thickness rotator cuff repairs followed for 12 months showed no adverse outcomes.63

One pilot study reported using cultured human tenocytes in the treatment of tendinopathy without any adverse effects.30 However, the use of tenocytes is not within the scope of this report.

Back to Top | Article Outline

Safety in Muscle Injury

There are no data in the literature that addresses the safety of MSCs in human muscle injury.24 Questions have been raised about the possibility of heterotopic bone formation in injured muscle treated with MSCs.64–66

Back to Top | Article Outline

EFFICACY

There are promising studies that MSCs enhance tendon repair in rabbit,15 rat,67 and horse68 models.

Mesenchymal stem cells have been demonstrated both in vitro69 and in vivo70 to be able to differentiate into articular cartilage, thus suggesting that they may be useful for treating articular cartilage injuries.

There is biological plausibility that MSCs should be therapeutic in the management of osteoarthritis. Laboratory-based evidence demonstrates MSC mediated catabolic and inflammatory effects that should be chondroprotective.71,72

Osteoarthritis differs from an osteochondral defect. The former is a dynamic process where mechanical stressors and catabolic processes overwhelm the joints capacity for repair, whereas the latter can be an acute injury or metabolic condition. Mesenchymal stem cell research outcomes must be carefully considered before extrapolating from one pathology to the other.

Introducing standards to MSC treatment, for example, how many cells should be injected, will make it easier to analyze treatment outcomes but may lead to debate when companies are already marketing MSC products that have different processes and cell concentrates based on inadequate science.73–75

Animal models suggest that where MSCs are derived from tissue that is similar to the target organ, the better the outcomes. Human research suggests this is also a consideration. Adipose tissue offers a readily available source of MSCs, however, these tissues are dissimilar to musculoskeletal tissue. How adipose-derived MSCs compare with those derived from tissue similar to the target organ requires further research. Whether cells need culture or can be injected in same-day procedures also needs further investigation. How many MSCs are required to achieve any therapeutic outcome remains unanswered.76,77

Back to Top | Article Outline

Efficacy in Osteoarthritis

The most appropriate method to determine whether an intervention has a placebo effect is to undertake a double-blind placebo randomized control trial (level 2 evidence). To date, there are no published studies of this caliber.

There are several small39–41,43,47,78–86 and 1 large87 cohort study where injected MSCs showed improved pain and functional scores. Some reported evidence of arthroscopic or radiographic improvements to the articular cartilage at follow-up.41,43,47,78–82,85,86

There are several small studies comparing MSCs to hyaluronic acid—one 7 to 10 days post arthroscopic debridement,47 the others not related to surgery.47,88 Although the MSCs were better than hyaluronic acid in the surgical group and equivalent to hyaluronic acid in the nonsurgical group, it is difficult to fully interpret these results because of the nature of the comparison groups. Arthroscopic debridement surgery has been shown to worsen patient outcomes in knee arthritis,89,90 and it remains unproven whether hyaluronic acid is therapeutic.91 It is not known whether MSCs ameliorate the detrimental effect of surgery and allow more acceptable surgical outcomes. Likewise, comparing MSCs to a treatment with unproven outcomes is challenging to interpret.

The Osteoarthritis Stem Cell Advanced Research Study remains the only double-blind randomized placebo-controlled trial using injected MSCs for knee osteoarthritis. It demonstrates no difference in efficacy between the treatment group (receiving nonexpanded adipose-derived MSCs) and those who had MSCs harvested but received a saline control injection at any point out to 12 months follow-up.92,93 Although published as a conference abstract,93 the full study remains unpublished in a peer reviewed journal.

Back to Top | Article Outline

Efficacy in Tendinopathy

Although there are a large number of articles looking at animal tendinopathies, many use collagenase-induced injuries rather than the clinical scenario seen in humans of overuse or age-related tendon degeneration.24 Two studies examining the equine equivalent of Achilles tendinopathy demonstrated a decrease in tendon reinjury rates compared with historical veterinary data.24 Animal models suggest that MSCs alone have less efficacy without the presence of a biological “scaffold” and/or growth factors. This would suggest that without the scaffold and cytokine influence the MSC effect is predominantly paracrine rather than the MSCs themselves integrating into the repair.16,24,94–99 A review article (2013) was unable to find any completed clinical studies using autologous MSCs in tendon repair.24

Back to Top | Article Outline

Efficacy in Muscle Injury

Mesenchymal stem cells exist in skeletal muscle. Homeostasis and healing/repair of muscle is complex.100 To date, no literature has examined autologous MSC use in muscle healing.24

Back to Top | Article Outline

ETHICAL CONCERNS REGARDING MESENCHYMAL STEM CELL USE IN MUSCULOSKELETAL CONDITIONS

The underlying ethical values important to the everyday work of sports physicians are also pertinent when considering the use of MSCs for musculoskeletal conditions. Respect for patient autonomy while promoting benefit and avoiding harm, and acting in a fair and just manner are some of the important ethical concepts that can be used to usefully reflect on this relatively new area of work.101–107

New developments in health care have the potential to benefit many. However, patients can be harmed as a result of the introduction of new therapies with insufficient evidence to support their widespread use.35–38,89,90,108 Therefore, good quality research is needed to ensure that innovations are safe and efficacious before wider clinical use.106,108,109 A balance must be struck that allows for a level of innovation, yet ensures that patient safety is paramount through the use of robust research to ensure safety and efficacy.

Mesenchymal stem cell therapy is an area of collaboration between the medical profession, and commercial and noncommercial interdisciplinary groups in an area where the science is preliminary, not all the evidence is in the public domain and the regulations are evolving.66,92,93 Other countries have developed regulations to protect the consumer110–114 but adherence to those regulations have varied.75,110,112,115–117 On some occasions, clinicians have ignored practice constraints and others have exploited regulatory “loopholes”21,116 allowing treatments to be marketed to the public bypassing standards for innovative therapies coming to market.

The use of autologous MSCs for the treatment of musculoskeletal conditions is currently unproven and therefore has not yet been determined to be safe or efficacious for clinical use, and there is a lack of credible long-term data to support clinical application.21,114,115,118,119 Some of the risks and burdens associated with MSC use are well recognized because they arise by virtue of their route of administration (including injection-site infection and wrong-site administration).21,112 Other aspects of MSC use where there are, as of yet, no clear treatment guidelines may pose risks and potential harms that are yet to be determined, including cell preparation, dose, and frequency,12 and any long-term harms that may eventuate.21 There is therefore, a need to protect the patient from known and as yet unknown harms arising from new and unproven interventions. Not only must we consider the potential harms to an individual patient or research participant, but harms to the integrity of the medical profession and erosion of public trust in medical research116 through the unchecked practices of some clinics undertaking MSC therapy. Yet, demand exists for new treatments in musculoskeletal care, particularly where the patient has few clinical options remaining.91,106,120 Athletes and physically active members of the community seek rapid resolution of their musculoskeletal problems. Consequently, they may be willing to accept treatment options that have not been subjected to full clinical scrutiny, in an endeavor to obtain accelerated “return to play.” These factors place pressure on doctors to consider new therapies where options are limited, but they also potentially leave patients vulnerable to exploitation.74

In recent years, there has been a proliferation of clinics offering,116 and sometimes actively marketing, MSCs for treatment of several musculoskeletal problems. Some clinics have overstated claims about the efficacy and safety of MSCs given the current knowledge.75,115,116,119,121 Concern has also been raised about the high cost of care, the potential conflicts of interest clinicians might have, and the claims made in advertising given the absence of proven therapeutic benefit. Calls have been made for this area to be more stringently regulated.21,115,116

So far, research in this area has revolved around autologous MSCs. Where research moves toward MSC allografts, the ethical considerations are more complicated and attract regulations to protect patients.122

There are also concerns that patients may travel outside their home jurisdiction to receive MSC therapy where regulatory requirements are less rigorous. Australasian College of Sports Physicians members need to be cognizant of these concerns and to the pressures this activity may place on them.74,101

Back to Top | Article Outline

Advertising

As stated earlier, patients seeking MSC therapy have frequently reached the end of conventional treatment options; this may leave them particularly vulnerable to exploitation through the use of unwarranted claims of efficacy beyond what is currently known, or through testimonials from celebrity patients.112,116,123

In both Australia and New Zealand, there is a clear expectation that advertisements or claims made about treatment must meet particular expectations. The Medical Board of Australia Code of Conduct104 states that:

Good Medical Practice Involves:

  • 8.6.1 Making sure that any information you publish about your medical services is factual and verifiable.
  • 8.6.2 Making only justifiable claims about the quality or outcomes of your services in any information you provide to patients.
  • 8.6.3 Not guaranteeing cures, exploiting patients' vulnerability or fears about their future health, or raising unrealistic expectations.
  • 8.6.4 Not offering inducements or using testimonials.

The New Zealand Medical Council statement on advertising states makes similar demands, requiring that claims about services are factual and verifiable, and that people are not encouraged to have unrealistic expectations.124 The New Zealand Medical Association Code of Ethics specifically states that testimonials and endorsements not be used.125

Back to Top | Article Outline

THE ACSP POSITION

The position adopted by the ACSP promotes patient safety and autonomy, endeavors to reduce potential harm to the patient, provides a level of clarity in an area of uncertainty, and takes responsibility for the advancement of knowledge.101,106,122 The ACSP position protects patients and the profession in the absence of unequivocal evidence regarding effectiveness and safety of these techniques, and yet responds to a recognized need to actively contribute to the development of knowledge in this area.101,126 The ACSP believes that robust knowledge generation is vital to improving patient care.106

The ACSP believes that any use of MSCs for musculoskeletal conditions must fit within either of the following pathways:

  1. As part of a rigorous clinical research trial.
  2. As an individualized innovative therapy where there is a commitment to the transparent collection of data for analysis by a research body external to the clinic.
Back to Top | Article Outline

As Part of a Rigorous Clinical Research Trial

The ACSP encourages the establishment of research studies to determine the safety and efficacy of MSC for the treatment of musculoskeletal conditions. Mesenchymal stem cell use for musculoskeletal conditions as part of a clinical research trial must be registered with an appropriate national or international clinical research trial registry.107,114,127,128 Any research trial must be subject to rigorous and independent scientific peer review before start of the study, and receive ethical approval from an accredited human research ethics committee.106,107,129 It is expected that any and all research findings are shared within the scientific and medical community including adverse outcomes.106,112,129

Back to Top | Article Outline

As An Individualized Innovative Therapy Where There Is a Commitment to the Transparent Collection of Data for Analysis by a Research Body External to the Clinic

Innovation carries an expectation of improved patient care, and duties to the medical profession, and society at large. Duties include contributions to knowledge, collaboration with peers, and information sharing.130

As the state of knowledge currently held about MSC for musculoskeletal conditions is limited, offering this intervention beyond a clinical research trial would be considered to be innovative treatment.21,106,115,116 Innovative therapies are new or modified treatments undertaken in the best interests of the patient but with, as of yet, unproven outcomes. Innovative practices are essential for medical progress,130 but should be subject to formal evaluation through rigorous peer-reviewed research.109 In the absence of robust research, innovative practices may become widely adopted without adequate data to determine risks and benefit; physicians are unable to determine safety and efficacy; and informed consent will be compromised.130

The clinical use of MSCs as innovative therapy must be recorded, including indications for treatment, clinical and demographic data, and clinical outcomes. These should be open to scrutiny and auditable as part of a commitment to knowledge generation and improving care.131 Australasian College of Sports Physicians members should be willing to engage in practices that permit open and transparent scientific evaluation of such services by an independent external research body (analogous to the Australian and New Zealand Orthopedic joint replacement registries132,133). The research body will perform scientific analysis of data. It would be expected that only small numbers of patients would go through this pathway, and would receive MSC as part of individualized care decided on a case-by-case basis.21,130 Clinicians using MSCs as an innovative therapy should move to clinical trials in a timely fashion after experience with at most a few patients.21,130

Back to Top | Article Outline

Informed Consent

In the interests of respecting patient autonomy, ACSP members will be required to inform patients about all aspects of their care,106 including whether they are part of a research trial, or are receiving innovative therapy.103

Any patients receiving MSC therapy under either pathway need to be made aware of and presented in writing the following:

  1. That MSCs are experimental and have not yet been proven to be safe or effective for clinical use.
  2. The long-term harms from the use of MSCs have not been determined.
  3. That the patient is being offered a therapy that has not been validated through reliable research methods.
  4. That identifiable personal patient or participant information and treatment will be entered in a database and accessed by researchers.
  5. That patients may be contacted at a later date for research purposes.
  6. That ethical approval will be sought before accessing patient data.
  7. Any conflicts of interest (including financial) held by the researcher or clinician providing innovative therapy will be declared.
  8. The full cost of the procedure, including a full breakdown of costs will be provided to the patient.
  9. Costs involved in MSC interventions used within research will not be passed onto participants as this has the potential to distort the research process.21,134
  10. Informed consent to the procedure or to participation in research will be obtained in writing.

All advertising for services must meet the standards of the Australian Medical Council and the Medical Council of New Zealand.

This position statement will be reviewed and updated if necessary in 2017.

Back to Top | Article Outline

AUSTRALASIAN COLLEGE OF SPORTS PHYSICIANS POSITION STATEMENT EXECUTIVE SUMMARY—MESENCHYMAL STEM CELLS IN SPORT AND EXERCISE MEDICINE

  1. Mesenchymal stem cell therapies are still under investigation.
  2. Research evidence to date suggests MSCs may be safe in the treatment of osteoarthritis and tendinopathies so that it is reasonable to proceed to further robust clinical trials with rigorous long-term follow-up.
  3. There is limited evidence that suggests that nonexpanded MSC therapies do not work. Further research is required to determine the safety and efficacy of expanded MSCs with and without biological scaffolds/growth factors.
  4. There is currently insufficient evidence from high-quality clinical trials to recommend the clinical use of MSC therapies for joint or tendon regeneration.
  5. The ACSP believes that robust knowledge generation is vital to improving patient care.
  6. The ACSP encourages the establishment of research studies to determine the safety and efficacy of MSCs for the treatment of musculoskeletal conditions.
    • Clinical research trials must be registered with an appropriate clinical research trial registry.
    • Any research trial must be subjected to peer review, and receive human research ethics committee approval.
    • Any and all research findings will be shared within the scientific and medical community including adverse outcomes.
  7. The ACSP position promotes patient safety and autonomy, endeavors to reduce potential harm to the patient, provides a level of clarity in an area of uncertainty, and takes responsibility for the advancement of knowledge.
  8. The ACSP position protects patients and the profession in the absence of unequivocal evidence regarding effectiveness and safety of these techniques, and yet responds to a recognized need to actively contribute to the development of knowledge in this area.
  9. The ACSP believes that any use of MSCs for musculoskeletal conditions must fit within either of the following pathways:
    • As part of a rigorous clinical research trial.
    • As an individualized innovative therapy. It is expected that only small numbers of patients would go through this pathway.
  10. The use of MSCs must only be undertaken within the expectations of the relevant medical regulatory organizations.
  11. Australasian College of Sports Physicians members should engage in practices that permit open and transparent scientific evaluation of such services by an independent external research body.
  12. All indications for treatment, clinical, and demographic data, and clinical outcomes should be open to scrutiny as part of a commitment to increasing knowledge and improving care.
  13. Australasian College of Sports Physicians members must inform all patients receiving MSC therapy that:
    • They are part of a research trial, or are receiving innovative therapy.
    • Mesenchymal stem cells are experimental and have not yet been proven to be safe or effective for clinical use.
    • The long-term harms from the use of MSCs have not been determined.
    • Identifiable personal patient or participant information and treatment will be entered to a database and accessed by researchers.
    • They may be contacted at a later date for research purposes.
    • Ethical approval will be sought before accessing patient data.
    • Any conflicts of interest held by the researcher or clinician providing innovative therapy will be declared.
    • The full cost of the procedure, including a full breakdown will be provided to the patient. Costs involved in MSC interventions used within research will not be passed onto participants.
    • Informed consent to the procedure will be obtained in writing.
Back to Top | Article Outline

ACKNOWLEDGMENTS

Thanks to Alison K. McLean, BSc (Hons) LLB (Hons), Sydney Medical School, University of Sydney and Grant R. Gillett, Professor of Biomedical Ethics, University of Otago for their invaluable contributions to this article.

Back to Top | Article Outline

REFERENCES

1. Dimarino AM, Caplan AI, Bonfield TL. Mesenchymal stem cells in tissue repair. Front Immunol. 2013;4:201.
2. Dominici M, Le Blanc K, Mueller I, et al.. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy. 2006;8:315–317.
3. Perez-Ilzarbe M, Diez-Campelo M, Aranda P, et al.. Comparison of ex vivo expansion culture conditions of mesenchymal stem cells for human cell therapy. Transfusion. 2009;49:1901–1910.
4. Ankrum J, Karp JM. Mesenchymal stem cell therapy: two steps forward, one step back. Trends Mol Med. 2010;16:203–209.
5. Banfi A, Muraglia A, Dozin B, et al.. Proliferation kinetics and differentiation potential of ex vivo expanded human bone marrow stromal cells: implications for their use in cell therapy. Exp Hematol. 2000;28:707–715.
6. Bonab MM, Alimoghaddam K, Talebian F, et al.. Aging of mesenchymal stem cell in vitro. BMC Cell Biol. 2006;7:14.
7. LaBarge MA, Blau HM. Biological progression from adult bone marrow to mononucleate muscle stem cell to multinucleate muscle fiber in response to injury. Cell. 2002;111:589–601.
8. Phinney DG, Prockop DJ. Concise review: mesenchymal stem/multipotent stromal cells: the state of transdifferentiation and modes of tissue repair–current views. Stem Cells. 2007;25:2896–2902.
9. Veronesi F, Giavaresi G, Tschon M, et al.. Clinical use of bone marrow, bone marrow concentrate, and expanded bone marrow mesenchymal stem cells in cartilage disease. Stem Cells Dev. 2013;22:181–192.
10. Sampson S, Botto-van Bemden A, Aufiero D. Autologous bone marrow concentrate: review and application of a novel intra-articular orthobiologic for cartilage disease. Phys Sportsmed. 2013;41:7–18.
11. Eaves FF III. Got evidence? Stem cells, bias, and the level of evidence ladder: commentary on: “ASAPS/ASPS position statement on stem cells and fat grafting”. Aesthet Surg J. 2011;31:718–722.
12. Yu SP, Hunter DJ. Emerging drugs for the treatment of knee osteoarthritis. Expert Opin Emerg Drugs. 2015;20:1–18.
13. Casiraghi F, Remuzzi G, Abbate M, et al.. Multipotent mesenchymal stromal cell therapy and risk of malignancies. Stem Cell Rev. 2013;9:65–79.
14. Lalu MM, McIntyre L, Pugliese C, et al.. Safety of cell therapy with mesenchymal stromal cells (SafeCell): a systematic review and meta-analysis of clinical trials. PLoS One. 2012;7:e47559.
15. Chong AK, Ang AD, Goh JC, et al.. Bone marrow-derived mesenchymal stem cells influence early tendon-healing in a rabbit achilles tendon model. J Bone Joint Surg Am. 2007;89:74–81.
16. Longo UG, Lamberti A, Maffulli N, et al.. Tissue engineered biological augmentation for tendon healing: a systematic review. Br Med Bull. 2011;98:31–59.
17. Young RG, Butler DL, Weber W, et al.. Use of mesenchymal stem cells in a collagen matrix for Achilles tendon repair. J Orthop Res. 1998;16:406–413.
18. Wei CC, Lin AB, Hung SC. Mesenchymal stem cells in regenerative medicine for musculoskeletal diseases: bench, bedside, and industry. Cell Transplant. 2014;23:505–512.
19. Bongso A, Fong CY, Gauthaman K. Taking stem cells to the clinic: major challenges. J Cell Biochem. 2008;105:1352–1360.
20. International Society for Stem Cell Research. The Guidelines for the Clinical Translation of Stem Cells. 2008. http://www.isscr.org/docs/guidelines/isscrglclinicaltrans.pdf. Accessed July 31, 2015.
21. International Society for Stem Cell Research. Statement on Delivery of Unproven Autologous Cell-based Interventions to Patients. 2013. http://www.isscr.org/docs/default-source/isscr-statements/isscr-acbistatement-091113-fl.pdf. Accessed July 22, 2015.
22. National Institue of Health. Glossary of Terms. 2001. http://grants.nih.gov/grants/peer/tree_glossary.pdf. Accessed August 3, 2015.
23. National Health and Medical Research Council. Phases of Clinical Trials. 2015. http://www.australianclinicaltrials.gov.au/what-clinical-trial/phases-clinical-trials. Accessed August 5, 2015.
24. Davies BM, Morrey ME, Mouthuy PA, et al.. Repairing damaged tendon and muscle: are mesenchymal stem cells and scaffolds the answer? Regen Med. 2013;8:613–630.
25. Gomez-Barrena E, Sola CA, Bunu CP. Regulatory authorities and orthopaedic clinical trials on expanded mesenchymal stem cells. Int Orthop. 2014;38:1803–1809.
26. Jung JW, Kwon M, Choi JC, et al.. Familial occurrence of pulmonary embolism after intravenous, adipose tissue-derived stem cell therapy. Yonsei Med J. 2013;54:1293–1296.
27. Pytel P, Husain A, Moskowitz I, et al.. Ventricular fibrillation following autologous intramyocardial cell therapy for inherited cardiomyopathy. Cardiovasc Pathol. 2010;19:e33–e36.
28. Kishk NA, Abokrysha NT, Gabr H. Possible induction of acute disseminated encephalomyelitis (ADEM)-like demyelinating illness by intrathecal mesenchymal stem cell injection. J Clin Neurosci. 2013;20:310–312.
29. Dlouhy BJ, Awe O, Rao RC, et al.. Autograft-derived spinal cord mass following olfactory mucosal cell transplantation in a spinal cord injury patient. J Neurosurg Spine. 2014;21:618–622.
30. Wang A, Breidahl W, Mackie KE, et al.. Autologous tenocyte injection for the treatment of severe, chronic resistant lateral epicondylitis: a pilot study. Am J Sports Med. 2013;41:2925–2932.
31. Pan Q, Fouraschen SM, de Ruiter PE, et al.. Detection of spontaneous tumorigenic transformation during culture expansion of human mesenchymal stromal cells. Exp Biol Med (Maywood). 2014;239:105–115.
32. Peeters CM, Leijs MJ, Reijman M, et al.. Safety of intra-articular cell-therapy with culture-expanded stem cells in humans: a systematic literature review. Osteoarthritis Cartilage. 2013;21:1465–1473.
33. Pascual-Garrido C, Rolo[Combining Acute Accent]n A, Makino A. Treatment of chronic patellar tendinopathy with autologous bone marrow stem cells: a 5-year-followup. Stem Cells Int. 2012;2012:953510.
34. Singh A, Gangwar DS, Singh S. Bone marrow injection: a novel treatment for tennis elbow. J Nat Sci Biol Med. 2014;5:389–391.
35. Curfman GD, Morrissey S, Drazen JM. Expression of concern reaffirmed. N Engl J Med. 2006;354:1193.
36. Bombardier C, Laine L, Reicin A, et al.. Comparison of upper gastrointestinal toxicity of rofecoxib and naproxen in patients with rheumatoid arthritis. VIGOR Study Group. N Engl J Med. 2000;343:1520–1528, 1522 p following 1528.
37. Unzueta A, Vargas HE. Nonsteroidal anti-inflammatory drug-induced hepatoxicity. Clin Liver Dis. 2013;17:643–656, ix.
38. Hawkey CJ, Weinstein WM, Stricker K, et al.. Clinical trial: comparison of the gastrointestinal safety of lumiracoxib with traditional nonselective nonsteroidal anti-inflammatory drugs early after the initiation of treatment–findings from the Therapeutic Arthritis Research and Gastrointestinal Event Trial. Aliment Pharmacol Ther. 2008;27:838–845.
39. Centeno CJ, Schultz JR, Cheever M, et al.. Safety and complications reporting update on the re-implantation of culture-expanded mesenchymal stem cells using autologous platelet lysate technique. Curr Stem Cell Res Ther. 2011;6:368–378.
40. Davatchi F, Abdollahi BS, Mohyeddin M, et al.. Mesenchymal stem cell therapy for knee osteoarthritis. Preliminary report of four patients. Int J Rheum Dis. 2011;14:211–215.
41. Emadedin M, Aghdami N, Taghiyar L, et al.. Intra-articular injection of autologous mesenchymal stem cells in six patients with knee osteoarthritis. Arch Iran Med. 2012;15:422–428.
42. Haleem AM, Singergy AA, Sabry D, et al.. The clinical use of human culture-expanded autologous bone marrow mesenchymal stem cells transplanted on platelet-rich fibrin glue in the treatment of articular cartilage defects: a pilot study and preliminary results. Cartilage. 2010;1:253–261.
43. Lee KB, Wang VT, Chan YH, et al.. A novel, minimally-invasive technique of cartilage repair in the human knee using arthroscopic microfracture and injections of mesenchymal stem cells and hyaluronic acid—a prospective comparative study on safety and short-term efficacy. Ann Acad Med Singapore. 2012;41:511–517.
44. Wakitani S, Okabe T, Horibe S, et al.. Safety of autologous bone marrow-derived mesenchymal stem cell transplantation for cartilage repair in 41 patients with 45 joints followed for up to 11 years and 5 months. J Tissue Eng Regen Med. 2011;5:146–150.
45. Teo BJ, Buhary K, Tai BC, et al.. Cell-based therapy improves function in adolescents and young adults with patellar osteochondritis dissecans. Clin Orthop Relat Res. 2013;471:1152–1158.
46. Kasemkijwattana C, Hongeng S, Kesprayura S, et al.. Autologous bone marrow mesen- chymal stem cells implantation for cartilage defects: two cases report. J Med Assoc Thai. 2011;94:395–400.
47. Vangsness CT Jr, Farr J II, Boyd J, et al.. Adult human mesenchymal stem cells delivered via intra-articular injection to the knee following partial medial meniscectomy: a randomized, double-blind, controlled study. J Bone Joint Surg Am. 2014;96:90–98.
48. Pak J, Chang JJ, Lee JH, et al.. Safety reporting on implantation of autologous adipose tissue-derived stem cells with platelet-rich plasma into human articular joints. BMC Musculoskelet Disord. 2013;14:337.
49. Kim YS, Park EH, Kim YC, et al.. Clinical outcomes of mesenchymal stem cell injection with arthroscopic treatment in older patients with osteochondral lesions of the talus. Am J Sports Med. 2013;41:1090–1099.
50. Kim YS, Lee HJ, Choi YJ, et al.. Does an injection of a stromal vascular fraction containing adipose-derived mesenchymal stem cells influence the outcomes of marrow stimulation in osteochondral lesions of the talus? A clinical and magnetic resonance imaging study. Am J Sports Med. 2014;42:2424–2434.
51. Giannini S, Buda R, Vannini F, et al.. One-step bone marrow-derived cell transplantation in talar osteochondral lesions. Clin Orthop Relat Res. 2009;467:3307–3320.
52. Giannini S, Buda R, Battaglia M, et al.. One-step repair in talar osteochondral lesions: 4-year clinical results and t2-mapping capability in outcome prediction. Am J Sports Med. 2013;41:511–518.
53. Wakitani S, Nawata M, Tensho K, et al.. Repair of articular cartilage defects in the patello-femoral joint with autologous bone marrow mesenchymal cell transplantation: three case reports involving nine defects in five knees. J Tissue Eng Regen Med. 2007;1:74–79.
54. Saw KY, Anz A, Siew-Yoke Jee C, et al.. Articular cartilage regeneration with autologous peripheral blood stem cells versus hyaluronic acid: a randomized controlled trial. Arthroscopy. 2013;29:684–694.
55. Koh YG, Kwon OR, Kim YS, et al.. Comparative outcomes of open-wedge high tibial osteotomy with platelet-rich plasma alone or in combination with mesenchymal stem cell treatment: a prospective study. Arthroscopy. 2014;30:1453–1460.
56. Wakitani S, Imoto K, Yamamoto T, et al.. Human autologous culture expanded bone marrow mesenchymal cell transplantation for repair of cartilage defects in osteoarthritic knees. Osteoarthritis Cartilage. 2002;10:199–206.
57. Wong KL, Lee KB, Tai BC, et al.. Injectable cultured bone marrow-derived mesenchymal stem cells in varus knees with cartilage defects undergoing high tibial osteotomy: a prospective, randomized controlled clinical trial with 2 years' follow-up. Arthroscopy. 2013;29:2020–2028.
58. Silva A, Sampaio R, Fernandes R, et al.. Is there a role for adult non-cultivated bone marrow stem cells in ACL reconstruction? Knee Surg Sports Traumatol Arthrosc. 2014;22:66–71.
59. Koh YG, Choi YJ, Kwon OR, et al.. Second-Look arthroscopic evaluation of cartilage lesions after mesenchymal stem cell implantation in osteoarthritic knees. Am J Sports Med. 2014;42:1628–1637.
60. Filardo G, Madry H, Jelic M, et al.. Mesenchymal stem cells for the treatment of cartilage lesions: from preclinical findings to clinical application in orthopaedics. Knee Surg Sports Traumatol Arthrosc. 2013;21:1717–1729.
61. Nejadnik H, Hui JH, Feng Choong EP, et al.. Autologous bone marrow–derived mesenchymal stem cells versus autologous chondrocyte implantation: an observational cohort study. Am J Sports Med. 2010;38:1110–1116.
62. Buda R, Vannini F, Cavallo M, et al.. Osteochondral lesions of the knee: a new one-step repair technique with bone-marrow-derived cells. J Bone Joint Surg Am. 2010;92(suppl 2):2–11.
63. Ellera Gomes JL, da Silva RC, Silla LM, et al.. Conventional rotator cuff repair complemented by the aid of mononuclear autologous stem cells. Knee Surg Sports Traumatol Arthrosc. 2012;20:373–377.
64. Rothrauff BB, Tuan RS. Cellular therapy in bone-tendon interface regeneration. Organogenesis. 2014;10:13–28.
65. Steinert AF, Rackwitz L, Gilbert F, et al.. Concise review: the clinical application of mesenchymal stem cells for musculoskeletal regeneration: current status and perspectives. Stem Cells Transl Med. 2012;1:237–247.
66. Grange S. Current issues and regulations in tendon regeneration and musculoskeletal repair with mesenchymal stem cells. Curr Stem Cell Res Ther. 2012;7:110–114.
67. Huang TF, Yew TL, Chiang ER, et al.. Mesenchymal stem cells from a hypoxic culture improve and engraft Achilles tendon repair. Am J Sports Med. 2013;41:1117–1125.
68. Godwin EE, Young NJ, Dudhia J, et al.. Implantation of bone marrow-derived mesenchymal stem cells demonstrates improved outcome in horses with overstrain injury of the superficial digital flexor tendon. Equine Vet J. 2012;44:25–32.
69. Ma HL, Hung SC, Lin SY, et al.. Chondrogenesis of human mesenchymal stem cells encapsulated in alginate beads. J Biomed Mater Res A. 2003;64:273–281.
70. Ma HL, Chen TH, Low-Tone Ho L, et al.. Neocartilage from human mesenchymal stem cells in alginate: implied timing of transplantation. J Biomed Mater Res A. 2005;74:439–446.
71. Platas J, Guillen MI, del Caz MD, et al.. Conditioned media from adipose-tissue-derived mesenchymal stem cells downregulate degradative mediators induced by interleukin-1beta in osteoarthritic chondrocytes. Mediators Inflamm. 2013;2013:357014.
72. Manferdini C, Maumus M, Gabusi E, et al.. Adipose-derived mesenchymal stem cells exert antiinflammatory effects on chondrocytes and synoviocytes from osteoarthritis patients through prostaglandin E2. Arthritis Rheum. 2013;65:1271–1281.
73. Shen H. Stricter standards sought to curb stem-cell confusion. Nature. 2013;499:389.
74. Zarzeczny A, Clark M. Unproven stem cell-based interventions & physicians' professional obligations; a qualitative study with medical regulatory authorities in Canada. BMC Med Ethics. 2014;15:75.
75. Munsie M, Hyun I. A question of ethics: selling autologous stem cell therapies flaunts professional standards. Stem Cell Res. 2014;13:647–653.
76. Centeno CJ. Clinical challenges and opportunities of mesenchymal stem cells in musculoskeletal medicine. PM R. 2014;6:70–77.
77. Bashir J, Sherman A, Lee H, et al.. Mesenchymal stem cell therapies in the treatment of musculoskeletal diseases. PM R. 2014;6:61–69.
78. Pak J, Lee JH, Lee SH. A novel biological approach to treat chondromalacia patellae. PLoS One. 2013;8:e64569.
79. Orozco L, Munar A, Soler R, et al.. Treatment of knee osteoarthritis with autologous mesenchymal stem cells: a pilot study. Transplantation. 2013;95:1535–1541.
80. Jo CH, Lee YG, Shin WH, et al.. Intra-articular injection of mesenchymal stem cells for the treatment of osteoarthritis of the knee: a proof-of-concept clinical trial. Stem Cells. 2014;32:1254–1266.
81. Saw KY, Anz A, Merican S, et al.. Articular cartilage regeneration with autologous peripheral blood progenitor cells and hyaluronic acid after arthroscopic subchondral drilling: a report of 5 cases with histology. Arthroscopy. 2011;27:493–506.
82. Orozco L, Munar A, Soler R, et al.. Treatment of knee osteoarthritis with autologous mesenchymal stem cells: two-year follow-up results. Transplantation. 2014;97:e66–68.
83. Centeno CJ, Schultz JR, Cheever M, et al.. Safety and complications reporting on the re-implantation of culture-expanded mesenchymal stem cells using autologous platelet lysate technique. Curr Stem Cell Res Ther. 2010;5:81–93.
84. Centeno CJ, Freeman MD. Percutaneous injection of autologous, culture-expanded mesenchymal stem cells into carpometacarpal hand joints: a case series with an untreated comparison group. Wien Med Wochenschr. 2014;164:83–87.
85. Koh YG, Jo SB, Kwon OR, et al.. Mesenchymal stem cell injections improve symptoms of knee osteoarthritis. Arthroscopy. 2013;29:748–755.
86. Koh YG, Choi YJ, Kwon SK, et al.. Clinical results and second-look arthroscopic findings after treatment with adipose-derived stem cells for knee osteoarthritis. Knee Surg Sports Traumatol Arthrosc. 2015;23:1308–1316.
87. Michalek J, Moster R, Lukac L, et al.. Autologous adipose tissue-derived stromal vascular fraction cells application in patients with osteoarthritis [published online ahead of print January 20, 2015]. Cell Transplant.
88. Vega A, Martin-Ferrero MA, Del Canto F, et al.. Treatment of knee osteoarthritis with allogeneic bone marrow mesenchymal stem cells: a randomized controlled trial. Transplantation. 2015;99:1681–1690.
89. Roos H, Lauren M, Adalberth T, et al.. Knee osteoarthritis after meniscectomy: prevalence of radiographic changes after twenty-one years, compared with matched controls. Arthritis Rheum. 1998;41:687–693.
90. Englund M, Lohmander LS. Risk factors for symptomatic knee osteoarthritis fifteen to twenty-two years after meniscectomy. Arthritis Rheum. 2004;50:2811–2819.
91. Hunter DJ. Viscosupplementation for osteoarthritis of the knee. N Engl J Med. 2015;372:1040–1047.
92. Osteoarthritis Stem Cell Advanced Research Study (OSCARS) Interim Report: A Randomised Double-blind, Placebo-controlled Trial of the Efficacy and Safety of Autologous Non-expanded Adipose-derived Stem Cells in the Treatment of Knee Osteoarthritis. Sydney, Australia: Regenius Ltd; 2013:1–6.
93. March L, Hunter DJ, Fedorova T, et al.. ARA oral abstracts—a randomised placebo controlled pilot study of autologous non-expanded adipose-derived mesenchymal stem cells in the treatment of knee osteoarthritis. Intern Med J. 2013;43:1–6.
94. Nixon AJ, Watts AE, Schnabel LV. Cell- and gene-based approaches to tendon regeneration. J Shoulder Elbow Surg. 2012;21:278–294.
95. Ahmad Z, Wardale J, Brooks R, et al.. Exploring the application of stem cells in tendon repair and regeneration. Arthroscopy. 2012;28:1018–1029.
96. Lui PP, Rui YF, Ni M, et al.. Tenogenic differentiation of stem cells for tendon repair-what is the current evidence? J Tissue Eng Regen Med. 2011;5:e144–e163.
97. Hogan MV, Bagayoko N, James R, et al.. Tissue engineering solutions for tendon repair. J Am Acad Orthop Surg. 2011;19:134–142.
98. Gott M, Ast M, Lane LB, et al.. Tendon phenotype should dictate tissue engineering modality in tendon repair: a review. Discov Med. 2011;12:75–84.
99. Tucker BA, Karamsadkar SS, Khan WS, et al.. The role of bone marrow derived mesenchymal stem cells in sports injuries. J Stem Cells. 2010;5:155–166.
100. Paylor B, Natarajan A, Zhang RH, et al.. Nonmyogenic cells in skeletal muscle regeneration. Curr Top Dev Biol. 2011;96:139–165.
101. Australasian College of Sports Physicians Code of Ethics and Professional Behaviour. 2008. http://acsp.org.au/acsp-policies/. Accessed July 31, 2015.
102. Australian Medical Association Code of Ethics. 2006. https://ama.com.au/position-statement/ama-code-ethics-2004-editorially-revised-2006. Accessed July 31, 2015.
103. New Zealand Medical Council. Good Medical Practice. 2013. https://www.mcnz.org.nz/news-and-publications/good-medical-practice/. Accessed July 31, 2015.
104. Medical Board of Australia. Good Medical Practice: A Code of Conduct for Docotrs in Australia 2014. http://www.medicalboard.gov.au/Codes-Guidelines-Policies/Code-of-conduct.aspx. Accessed July 31, 2015.
105. Beauchamp TL, Childress JF. Principles of Biomedical Ethics. 7th ed. Oxford, UK: Oxford University Press; 2012.
106. National Health and Medical Research Council National Statement on Ethical Conduct in Human Research. 2015. https://www.nhmrc.gov.au/guidelines-publications/e72. Accessed August 13, 2015.
107. Therapeutic Goods Administration. Note for Guidance on Good Clinical Practice CPMP/ICH/135/95. 2014. https://www.tga.gov.au/publication/note-guidance-good-clinical-practice. Accessed August 13, 2015.
108. McKinlay JB. From “Promising report” to “Standard procedure”: seven stages in the career of a medical innovation. Milbank Mem Fund Q Health Soc. 1981;59:374–411.
109. Lindvall O, Hyun I. Medical innovation versus stem cell tourism. Science. 2009;324:1664–1665.
110. Abbott A. Leaked files slam stem-cell therapy. Nature. 2014;505:139–140.
111. Anz AW, Hackel JG, Nilssen EC, et al.. Application of biologics in the treatment of the rotator cuff, meniscus, cartilage, and osteoarthritis. J Am Acad Orthop Surg. 2014;22:68–79.
112. Notarangelo LD. Into the wild–the use and abuse of stem cells in clinical practice. Am J Hematol. 2013;88:447–448.
113. Australian Government Therapeutic Goods Administration Legislation. 2015. http://www.tga.gov.au/excluded-goods-order-no-1-2011-guideline-items-4o-4p-4q-and-4r. Accessed August 5, 2015.
114. National Health and Medical Research Council. Stem Cell Treatments—A Quick Guide for Medical Practitioners Stem Cell Treatments. 2013. https://www.nhmrc.gov.au/guidelines-publications/rm001. Accessed July 31, 2015.
115. McLean AK, Stewart C, Kerridge I. Untested, unproven, and unethical: the promotion and provision of autologous stem cell therapies in Australia. Stem Cell Res Ther. 2015;6:33.
116. McLean AK, Stewart C, Kerridge I. The emergence and popularisation of autologous somatic cellular therapies in Australia: therapeutic innovation or regulatory failure? J Law Med. 2014;22:65–89.
117. Cyranoski D. Stem cells in Texas: cowboy culture. Nature. 2013;494:166–168.
118. The National Stem Cell Foundation of Australia and Stem Cells Australia. The Australian Stem Cell Handbook. 2015. http://www.stemcellfoundation.net.au/stem-cell-treatments-information/handbook. Accessed July 31, 2015.
119. National Health and Medical Research Council. Stem Cell Treatments—Frequently Asked Questions. 2013. http://www.nhmrc.gov.au/_files_nhmrc/publications/attachments/rm001a_stem_cell_treatments_faq_131220.pdf. Accessed August 13, 2015.
120. Messier SP, Mihalko SL, Legault C, et al.. Effects of intensive diet and exercise on knee joint loads, inflammation, and clinical outcomes among overweight and obese adults with knee osteoarthritis: the idea randomized clinical trial. JAMA. 2013;310:1263–1273.
121. Lau D, Ogbogu U, Taylor B, et al.. Stem cell clinics online: the direct-to-consumer portrayal of stem cell medicine. Cell Stem Cell. 2008;3:591–594.
122. Kirkpatrick B, Hercher L, Facio F, et al.. Stem cell research and therapy: the position of the National Society of Genetic Counselors. J Genet Couns. 2013;22:407–410.
123. Banja JD. Ethical considerations in stem cell research on neurologic and orthopedic conditions. PM R. 2015;7(4 suppl):S66–S75.
124. New Zealand Medical Council. Statement on Advertising. 2015. https://www.mcnz.org.nz/assets/News-and-Publications/Statements/Statement-on-advertising.pdf. Accessed July 31, 2015.
125. New Zealand Medical Association. Code of Ethics. 2013. https://www.nzma.org.nz/publications/code-of-ethics. Accessed July 22, 2015.
126. Eaves FF III, Haeck PC, Rohrich RJ. ASAPS/ASPS Position statement on stem cells and fat grafting. Plast Reconstr Surg. 2012;129:285–287.
127. Australian New Zealand Clinical Trials Registry. 2015. http://www.anzctr.org.au. Accessed August 6, 2015.
128. World Health Organisation International Clinical Trials Registry Platform. 2015. http://www.who.int/ictrp/en/. Accessed August 6, 2015.
129. National Health and Medical Research Council. Australian Code for the Responsible Conduct of Research. 2007. http://www.nhmrc.gov.au/guidelines-publications/r39. Accessed August 13, 2015.
130. American College of Obstetricians and Gynecologists Committee Opinion No. 352. Innovative Practice: Ethical Guidelines. Obstet Gynecol. 2006;108:1589–1595.
131. World Medical Association Declaration of Helsinki. Ethical Prinicples for Medical Research Involving Human Subjects. 2008. http://www.wma.net/en/30publications/10policies/b3/. Accessed August 20, 2015.
132. AOA National Joint Registry. 2015. https://aoanjrr.dmac.adelaide.edu.au. Accessed July 31, 2015.
133. NZOA Joint Registry. 2015. http://www.nzoa.org.nz/nz-joint-registry. Accessed July 31, 2015.
134. New Zealand Ministry of Health National Ethics Advisory Committee. Ethical Guidelines of Intervention Studies: Revised Edition. 2012. http://neac.health.govt.nz/system/files/documents/publications/ethical-guidelines-for-intervention-studies-2012v2_0.pdf. Accessed August 26, 2015.
    Copyright © 2016 Wolters Kluwer Health, Inc. All rights reserved.