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Immune Checkpoint Inhibition in Sepsis

A Phase 1b Randomized, Placebo-Controlled, Single Ascending Dose Study of Antiprogrammed Cell Death-Ligand 1 Antibody (BMS-936559)*

Hotchkiss, Richard S., MD1; Colston, Elizabeth, MD, PhD2; Yende, Sachin, MD3,4; Angus, Derek C., MD, MPH4; Moldawer, Lyle L., PhD5; Crouser, Elliott D., MD6; Martin, Greg S., MD, MSc, FCCM7; Coopersmith, Craig M., MD8; Brakenridge, Scott, MD, MSCS5; Mayr, Florian B., MD, MPH3,4; Park, Pauline K., MD9; Ye, June, PhD2; Catlett, Ian M., PhD2; Girgis, Ihab G., PhD2; Grasela, Dennis M., PharmD, PhD2

doi: 10.1097/CCM.0000000000003685
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Objectives: To assess for the first time the safety and pharmacokinetics of an antiprogrammed cell death-ligand 1 immune checkpoint inhibitor (BMS-936559; Bristol-Myers Squibb, Princeton, NJ) and its effect on immune biomarkers in participants with sepsis-associated immunosuppression.

Design: Randomized, placebo-controlled, dose-escalation.

Setting: Seven U.S. hospital ICUs.

Study Population: Twenty-four participants with sepsis, organ dysfunction (hypotension, acute respiratory failure, and/or acute renal injury), and absolute lymphocyte count less than or equal to 1,100 cells/μL.

Interventions: Participants received single-dose BMS-936559 (10–900 mg; n = 20) or placebo (n = 4) infusions. Primary endpoints were death and adverse events; key secondary endpoints included receptor occupancy and monocyte human leukocyte antigen-DR levels.

Measurements and Main Results: The treated group was older (median 62 yr treated pooled vs 46 yr placebo), and a greater percentage had more than 2 organ dysfunctions (55% treated pooled vs 25% placebo); other baseline characteristics were comparable. Overall mortality was 25% (10 mg dose: 2/4; 30 mg: 2/4; 100 mg: 1/4; 300 mg: 1/4; 900 mg: 0/4; placebo: 0/4). All participants had adverse events (75% grade 1–2). Seventeen percent had a serious adverse event (3/20 treated pooled, 1/4 placebo), with none deemed drug-related. Adverse events that were potentially immune-related occurred in 54% of participants; most were grade 1–2, none required corticosteroids, and none were deemed drug-related. No significant changes in cytokine levels were observed. Full receptor occupancy was achieved for 28 days after BMS-936559 (900 mg). At the two highest doses, an apparent increase in monocyte human leukocyte antigen-DR expression (> 5,000 monoclonal antibodies/cell) was observed and persisted beyond 28 days.

Conclusions: In this first clinical evaluation of programmed cell death protein-1/programmed cell death-ligand 1 pathway inhibition in sepsis, BMS-936559 was well tolerated, with no evidence of drug-induced hypercytokinemia or cytokine storm, and at higher doses, some indication of restored immune status over 28 days. Further randomized trials on programmed cell death protein-1/programmed cell death-ligand 1 pathway inhibition are needed to evaluate its clinical safety and efficacy in patients with sepsis.

1Department of Anesthesiology, Washington University School of Medicine, St Louis, MO.

2Department of Anesthesiology, Innovative Medicines Development, Bristol-Myers Squibb, Princeton, NJ.

3Veterans Affairs Pittsburgh Healthcare System, Pittsburgh, PA.

4The CRISMA Center, Department of Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PA.

5Department of Surgery, University of Florida College of Medicine, Gainesville, FL.

6Department of Medicine, The Ohio State University, Columbus, OH.

7Department of Medicine, Division of Pulmonary, Allergy, Critical Care & Sleep Medicine, Emory University, Atlanta, GA.

8Department of Surgery and Emory Critical Care Center, Emory University, Atlanta, GA.

9Department of Surgery, University of Michigan, Ann Arbor, MI.

*See also p. 733.

Supplemental digital content is available for this article. Direct URL citations appear in the printed text and are provided in the HTML and PDF versions of this article on the journal’s website (

Supported by Bristol-Myers Squibb.

Drs. Hotchkiss’s, Yende’s, Angus’s, Moldawer’s, Crouser’s, Martin’s, Coopersmith’s, Brakenridge’s, Mayr’s, and Park’s institution received funding from Bristol-Myers Squibb. Drs. Hotchkiss, Colston, Yende, Angus, and Moldawer received support for article research from Bristol-Myers Squibb. Drs. Hotchkiss, Colston, Moldawer, Crouser, Martin, Coopersmith, Brakenridge, Mayr, Park, Catlett, and Grasela disclosed off-label product use of antiprogrammed cell death-ligand 1 inhibitor (BMS-936559) for the treatment of immune suppression in the context of severe sepsis. Drs. Colston and Grasela disclosed they are shareholders of Bristol-Myers Squibb. Dr. Hotchkiss receives research grant support and serves on advisory boards to Bristol-Myers Squibb. Dr. Yende received grant support from Bristol-Myers Squibb for the design of this study. Dr. Angus received consulting fees from Bristol-Myers Squibb for advice on study design. Dr. Martin’s institution received funding from the National Institutes of Health. Dr. Coopersmith’s institution received funding from the Society of Critical Care Medicine (president in 2015). Dr. Park’s institution received funding from the National Institutes of Health, and she received other support from the U.S. Food and Drug Administration/Biomedical Advanced Research and Development Authority and Atox Bio. Drs. Colston, Ye, Catlett, Girgis, and Grasela disclosed that they are employees of Bristol-Myers Squibb.

Study registration number ( NCT02576457.

Data sharing: BMS policy on data sharing may be found at

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Sepsis is life-threatening organ dysfunction caused by a dysregulated host response to infection (1, 2), with high mortality rates worldwide (3, 4).

For years it was presumed that sepsis morbidity and mortality were secondary to an exaggerated systemic inflammatory response, but therapies intended to dampen this response failed to improve survival (5, 6). Although the causes of these failures are multifactorial, sepsis-associated immune dysregulation may play an important role (7, 8). Studies suggest that sepsis-associated immune dysregulation increases the risk of secondary infections and mortality (8–11).

Immune checkpoint pathways are endogenous components of the immune system that keep the immune response “in check” under normal physiologic conditions; tumor cells exploit these pathways to avoid recognition by the host. One of these immune checkpoint pathways is the programmed cell death protein-1 (PD-1)/programmed cell death-ligand 1 (PD-L1) pathway (12). PD-1 is a receptor that is inducibly expressed on T cells and functions as a negative regulator of T-cell function (12). Tumor cells express its primary ligand, PD-L1, which binds to PD-1 and triggers T-cell “inactivation”; PD-L1 expression on tumor cells is associated with poor prognosis (12, 13). Monoclonal antibodies (mAb) that block PD-1 and PD-L1 activity have proved highly successful at reducing tumor burden and are licensed for therapeutic use in patients with certain cancer types (14–18).

Immune dysregulation in sepsis bears similarities to that seen in certain cancers, particularly the up-regulation of the PD-1/PD-L1 pathway (19–21), and PD-1 and PD-L1 may be important in sepsis-associated immunosuppression. PD-1 and PD-L1 are also key mediators of T-cell exhaustion in infections (12, 22, 23); blocking their interaction prevents T-cell death, modulates cytokine production, and is associated with reduced organ dysfunction and fewer deaths in mice with cecal ligation and puncture-induced sepsis (24–27). PD-1 knockout mice also have marked protection against sepsis lethality versus wild-type mice (28). PD-1 and PD-L1 are also up-regulated on immune cells of patients with sepsis, and higher expression of these proteins is associated with increased mortality (9, 27–34). Furthermore, ex vivo studies using blood samples from patients with sepsis have reported decreased apoptosis and improved immune cell function with antibodies against PD-1 and PD-L1 (31, 33, 34). Therefore, anti–PD-L1 could be a promising approach for patients with sepsis-associated immunosuppression.

As with any agent that inhibits immune checkpoint pathways, there is a theoretical risk that this approach could induce an unbridled pro-inflammatory response or “cytokine storm.” Therefore, any novel agent under investigation in the oncology or sepsis fields should be monitored carefully for such a response. However, although animal and ex vivo sepsis studies have reported some cytokine changes with anti–PD-1 and anti–PD-L1 antibodies (e.g., increased interferon [INF]–γ, interleukin [IL]–6, and tumor necrosis factor-α), no excessive pro-inflammatory changes were reported (24–26, 31, 33). Furthermore, to the authors’ knowledge, no clinical finding of cytokine storm in patients with cancer receiving anti–PD-1 or anti–PD-L1 therapy has been reported.

BMS-936559 (Bristol-Myers Squibb, Princeton, NJ), an investigational, fully human immunoglobulin G4 mAb that inhibits binding of PD-L1 to both PD-1 and CD80, has been explored in phase 1 studies in individuals with cancer (35) and those with HIV-1 infection on suppressive antiviral therapy (36). Anti–PD-L1 was effective in augmenting host immunity, as indicated by its ability to induce tumor regression and prolong disease stabilization in patients with cancer, and to enhance HIV-1 Gag-specific CD8+ T-cell function, respectively (35, 36). The present study is the first clinical trial of checkpoint inhibitors in sepsis and represents a unique new immunotherapeutic approach to sepsis by targeting T-cell exhaustion and defective host adaptive immunity.

This study was undertaken primarily to evaluate the safety, tolerability, pharmacokinetics, and pharmacodynamics of anti–PD-L1 (BMS-936559) in patients with sepsis-associated immunosuppression. The study also explored biologic efficacy by examining the effects on immune system biomarkers.

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Ethics Statement

Written informed consent was obtained from all participants. Institutional Review Boards/Independent Ethics Committees approved the protocol and amendments. The study was conducted in accordance with Good Clinical Practice, as defined by the International Conference on Harmonisation, and in accordance with the ethical principles underlying European Union Directive 2001/20/EC and the United States Code of Federal Regulations, Title 21, Part 50 (21CFR50).

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Study Design and Population

This was a phase 1b, prospective, randomized, double-blind, placebo-controlled, multicenter study of BMS-936559 in adults with sepsis-associated immunosuppression ( identifier: NCT02576457), and was a sequential, single ascending-dose assessment at seven U.S. sites (December 2015–March 2017; supplementary information, Supplemental Digital Content 1,

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Inclusion Criteria.

Eligible participants were at least 18 years old with documented/suspected infection and sepsis onset at least 24 hours prior to study treatment administration based on one of three organ dysfunction criteria: hypotension (defined as treatment with any vasopressor[s] for at least 6 hr to maintain systolic pressure ≥ 90 mm Hg or mean arterial pressure ≥ 70 mm Hg), acute respiratory failure (mechanical ventilation for ≥ 24 hr), or acute kidney injury (creatinine > 2.0 mg/dL [from a normal pre-sepsis value] or urine output < 0.5 mL/kg/hr for > 2 hr despite adequate fluid resuscitation). Preexisting renal impairment required the participant to meet another organ dysfunction criterion. Participants were required to have sepsis-associated immunosuppression (in this study, operationally defined as absolute lymphocyte count [ALC] ≤ 1,100 cells/μL within the 96 hr before study treatment administration) (8, 11), and needed to be receiving treatment in an ICU.

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Exclusion Criteria.

Key criteria were a previous episode of sepsis with ICU admission during the current hospitalization, an advanced directive for withholding/withdrawing life-sustaining treatment/do not resuscitate order/comfort measures-only order, active autoimmune disease, history of transplantation, or cancer diagnosis or treatment in the preceding 6 months (supplementary information, Supplemental Digital Content 1,

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Treatment Assignment and Study Procedures.

Participants were assigned to a single dose of BMS-936559 or placebo; study treatment assignment was to one of five sequential groups (BMS-936559 10, 30, 100, 300, 900 mg). BMS-936559 was given as an IV infusion on day 1 (maximum infusion time: 180 min) (Fig. 1). The decision to wait at least 24 hours after the onset of organ dysfunction before starting treatment was taken in order to account for resolution of the peak pro-inflammatory response associated with sepsis (37).

Figure 1

Figure 1

All participants received standard-of-care therapy (38) and were followed for 90 days after dosing, unless they died or were lost to follow-up, or access to the participant was denied. After completing dosing in any group, subsequent group dosing was not initiated until blinded safety data through day 14 (or earlier for dosed participants who discontinued or died before day 14) in the earlier dose group(s) were reviewed and deemed acceptable by the sponsor’s Medical Monitor in consultation with the investigators. Doses in groups 4 (300 mg) and 5 (900 mg) were selected based on a review of safety, receptor occupancy (RO), and pharmacokinetic data available through day 14 from earlier groups. Study stopping rules are described in the supplementary information (Supplemental Digital Content 1,

Participants were randomized 4:1 (BMS-936559 to placebo) using a computer-generated randomization scheme, provided by the sponsor. The site pharmacist (who prepared the infusion) was notified of a participant’s treatment assignment. All other site staff and the sponsor remained blinded.

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Endpoints and Assessments

Key Objectives and Endpoints.

The primary objective was to assess safety and tolerability over 90 days following single-dose administration of BMS-936559 10–900 mg to participants with sepsis. Safety was assessed based on review of physical examination findings, vital sign measurements, electrocardiogram, adverse event (AE) reports, ophthalmoscopic examinations, and laboratory tests (for definitions of AEs and serious AEs [SAEs], see supplementary information, Supplemental Digital Content 1,

Key secondary objectives were to assess pharmacokinetics and RO of BMS-936559 following single-dose administration, and the effect of a single dose of BMS-936559 on immune function.

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Serial blood samples for pharmacokinetics/pharmacodynamics and biomarker analyses were collected at pre-dose and selected post-dose time points.

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BMS-936559 serum concentrations were measured using a validated enzyme-linked immunosorbent assay method (39). Pharmacokinetic variables (maximum observed serum concentration [Cmax], time of maximum observed serum concentration, area under the curve [AUC] from time 0 to time of the last measurable concentration after drug administration/extrapolated to infinity [AUC(0–T)/AUC(INF)], total body clearance, volume of distribution, and terminal half-life were derived by noncompartmental analysis using Phoenix WinNonlin, v6.3 or higher [Pharsight Corporation, Phoenix, Mountain View, CA]). Dose proportionality, based on Cmax, AUC(0–T), and AUC(INF), was assessed using the power model approach (40).

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BMS-936559 RO and Immune System Status.

PD-L1 RO on CD3+ T cells and immune system status (monocyte human leukocyte antigen [mHLA]-DR expression and ALC) up to day 90 were measured. In vitro data suggest that T-cell function (as assessed by INF-γ production [BMS data on file] or T-cell proliferation [41]), is dose-dependently enhanced by PD-1/PD-L1 blockade. PD-L1 RO on T cells and T-cell activity saturate/plateau similarly; at saturating RO, there is a plateau in INF-γ production (BMS data on file). BMS-936559 doses that achieved at least 80% RO were expected to restore or enhance T-cell function, and greater than or equal to 80% was thus regarded as a relevant RO level.

The mHLA-DR levels were assessed using whole blood in flow cytometry-based assays; an mHLA-DR level of less than 5,000 mAb/cell has been generally considered indicative of immunoparalysis (42). ALC was determined using a standard hematology analyzer. Cytokine levels (IL-6, IL-8 [CXCL8], and IL-10) were also measured up to day 90. IL-6 and IL-8 are markers of generalized immune system activation and inflammation, and IL-10 expression is considered an appropriate anti-inflammatory marker.

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Statistical Methods

The study employed a single ascending-dose design of four participants dosed with BMS-936559 and one with placebo at each dose level. Sample size was not based on statistical power considerations. Rather, if the incidence of an AE was 10%, then the sample size provided a 34.4% probability to observe at least one event in a given dose group.

Safety, pharmacokinetics, RO, and immunologic outcomes analyses were conducted on a modified intent-to-treat (ITT) population (ITT-exposed), comprising all participants who received at least a partial dose of study treatment. Descriptive statistics were used to summarize safety, pharmacokinetics, RO, and immunologic data.

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Participant Disposition and Baseline Characteristics

Thirty-five participants were enrolled, of whom 25 were randomized. Ten participants were excluded for the following reasons (in some cases, participants were excluded for more than one reason): one participant did not have documented or suspected infection; one participant had active autoimmune disease or documented history of autoimmune disease; three participants did not meet organ dysfunction criteria; six participants did not have ALC less than or equal to 1,100 cells/μL; five participants were not in the ICU at the time of study drug administration; and for one participant, the enrollment milestone had already been reached. One randomized participant’s status changed to “do not resuscitate” after randomization but prior to dosing, and so did not receive study treatment. Therefore, 24 participants received BMS-936559 (n = 20 [n = 4 per dose group]) or placebo (n = 4). Fourteen participants (58.3%) completed the 90-day study period (Supplementary Fig. S1, Supplemental Digital Content 1,

Baseline characteristics were comparable across groups, except for age and number of organ dysfunctions (Table 1).



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Six deaths occurred 2–52 days following BMS-936559 administration (Table 2); these were considered unrelated to study treatment by the investigator. Deaths occurred in two of four participants (50%) receiving BMS-936559 10 mg, two of four (50%) receiving 30 mg, one of four (25%) receiving 100 mg, and one of four (25%) receiving 300 mg (Table 2). The causes of death were not unexpected for these severely ill patients (Supplementary Table S1, Supplemental Digital Content 1,



SAEs occurred in four participants (16.7%; BMS-936559, n = 3/20 [15.0%]; placebo, n = 1/4 [25.0%]); all were considered unrelated to study treatment (Table 2; and Supplementary Table S2, Supplemental Digital Content 1, The most frequent (≥ 20%) on-treatment AEs (pooled BMS-936559 doses) were hypotension (n = 11; 55%), diarrhea and delirium (n = 7; 35% each), anemia (n = 6; 30%), increased lipase and pleural effusion (n = 5; 25% each), and decreased weight, hypokalemia, and malnutrition (n = 4; 20% each) (Table 2). Most AEs were mild to moderate (grade 1–2), with similar frequency and intensity across dose groups. The observed AEs were not unexpected for this population. One participant (BMS-936559 30 mg) had AEs considered related to study treatment: increased amylase (grade 2) and lipase (grade 1), and increased blood lactate dehydrogenase (LDH) (grade 1). The increased amylase and lipase events began approximately 24 hours after infusion and resolved after 5 days, with neither event requiring treatment. The increased blood LDH event began 6 days after infusion, resolved after approximately 14 hours, did not require treatment, and was not associated with hemolysis or anemia. No AEs led to discontinuation from the study.

AEs of special interest (AEOSIs, i.e., AEs with potential immune-related causes as identified in the anti–PD-L1 study in cancer patients [35]) occurred in 13 of 24 participants (54.2%; BMS-936559, n = 11/20 [55.0%]; placebo, n = 2/4 [50.0%]) (Table 3). Most were grade 1–2; none were deemed related to study treatment or were suggestive of a drug-induced exaggerated inflammatory response. Two participants (10%) had grade 3 AEOSIs of diarrhea: in one, the event began on day 7, resolved on day 12, was treated with loperamide, and did not require corticosteroids; in the other, the event began on day 6, resolved on day 23, was treated with diphenoxylate atropine, and did not require corticosteroids. One participant (5%) had a grade 3 AEOSI of lung infiltration, beginning 18 hours after study treatment infusion and resolving by 33 hours. It was treated with antibiotics and did not require corticosteroids. No cases of pneumonitis were reported.



Post-dose ophthalmoscopic data (at index hospitalization discharge and/or day 90) were available for 15 participants. No cases of focal retinal lesions were reported.

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The pharmacokinetic findings are presented and discussed in more detail in the supplementary information (see Supplementary Tables S3–S5 and Supplementary Fig. S2, Supplemental Digital Content 1, BMS-936559 mean terminal half-life ranged from 29 hours (10 mg) to 189 hours (300 mg). BMS-936559 exhibited nonlinear pharmacokinetics and target-mediated drug disposition kinetics, with faster elimination rates and higher volumes of distribution observed compared with patients with cancer. After dose-normalization, anti–PD-L1 observed concentrations were generally higher and decreased more slowly in participants with solid tumors than in participants with sepsis.

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A dose-dependent increase in RO duration was observed (Fig. 2A). There was also a dose-dependent increase in the time period over which greater than or equal to 80% RO was achieved; full RO was achieved for 28 days following the 900-mg single dose (Fig. 2A).

Figure 2

Figure 2

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Increased mHLA-DR expression over time was demonstrated, with the greatest increase observed at the two highest doses (Fig. 2B). From days 15 to 90, median mHLA-DR levels with BMS-936559 300 and 900 mg were greater than 5,000 mAb/cell (between ~6,000 and ~18,000 mAb/cell) (Fig. 2B).

A post hoc analysis combined the 10, 30, and 100 mg dose groups (“low-dose”) and 300 and 900 mg groups (“high-dose”) for RO and mHLA-DR to day 29 (Fig. 2C). Full RO was maintained until day 8 (low-dose) and until day 29 (high-dose). mHLA-DR expression rose above 5,000 mAb/cell at day 8 in the low-dose group and at day 4 in the high-dose group; beyond day 8, mHLA-DR expression was consistently higher at each time point in the high-dose versus low-dose group.

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Other Biomarkers.

No clear dose-related changes or trends were observed in ALC or cytokine levels (Supplementary Figs. S3–S5, Supplemental Digital Content 1,

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These data describe the first clinical evaluation of an anti–PD-L1 mAb in patients with sepsis-associated immunosuppression. Single doses of BMS-936559 (10–900 mg) were well tolerated. Most AEOSIs were of mild-to-moderate intensity, consistent with BMS-936559 mechanism of action, and generally similar to those reported in patients with cancer (rash, hypothyroidism, and diarrhea) (35). No cases of pneumonitis (a potentially life-threatening condition that has been recorded with anti–PD-1/PD-L1 mAb in the oncology setting [14–18]) were reported. As documented in the AE profile and cytokine measurements, there was no clinical or biomarker evidence of a drug-induced cytokine release syndrome. Specifically, there was no indication that administration of anti–PD-L1 was temporally associated with worsening fever or hemodynamic instability, and no observed clinically significant changes in cytokine concentrations. This is important because, although PD-1/PD-L1 pathway inhibition is effective (14–18), studies of therapies to boost immune activity in sepsis have not been performed, partly because of the theoretical risk of an excessive pro-inflammatory response (see introduction).

In BMS-936559 multiple-dose, 3-month primate toxicology studies, focal retinal lesions were reported, and monitoring was performed in the current clinical study. No focal retinal lesions were detected in any participant. This is consistent with the phase 1 BMS-936559 HIV-1 study, which did not reveal focal retinal findings similar to those seen in monkeys (36).

The pharmacokinetic findings of this study are interesting because the faster elimination rates and higher volumes of distribution seen compared with patients with cancer suggest that higher doses or more frequent dosing might be warranted for patients with sepsis. The duration of full RO increased dose-dependently, and the prolonged (28 d) RO at the 900-mg dose level could be advantageous in preventing new secondary infections that cause additional morbidity and mortality.

The mHLA-DR expression also appeared to increase over time, consistent with a restoration of immune function. The post hoc analysis highlighted the sustained, high degree of PD-L1 target engagement (RO) and implied a more rapid recovery of immune function (mHLA-DR expression) at the two higher doses than at the three lower doses. Due to small participant numbers, these data should be interpreted with caution. However, similar associations between immunotherapy and increased mHLA-DR expression (and improved outcome) have been reported elsewhere. Two INF-γ (multiple-dose) studies in patients with sepsis/trauma and immunoparalysis reported improved mHLA-DR expression and associated clinical improvements (43, 44). A study of granulocyte-macrophage colony-stimulating factor (multiple doses) versus placebo in patients with sepsis-associated immunosuppression reported mHLA-DR normalization, along with improved patient outcomes (45).

There was no clear trend in ALC levels after dosing with BMS-936559. Reduced ALC is a frequent and easily measured feature of sepsis-associated immunosuppression (8, 21), but may not be the optimal pharmacodynamic marker to assess the effect of PD-1/PD-L1 pathway inhibition in a short-term study. Lymphocyte counts may increase through homeostatic proliferation or de novo lymphocyte production; however, both processes would be expected to take a significant amount of time (46, 47). Thus, ALC may not fully recover over 90 days.

There were also no clear or dose-related trends in IL-6, IL-8, and IL-10 levels observed. Although animal/ex vivo studies have shown some changes in cytokine levels (24–26, 31, 33), there may be several reasons for a lack of a detectable change in markers in the current study. For example, the sampling times chosen may not have detected a rapid or transient change in cytokine level. Another reason may be that any substantial cytokine change was highly localized and not detectable systemically. The small sample size may also be a factor. However, there was an absence of any systemic change in cytokine levels indicative of a cytokine storm, which is an important “goal” from a first-in-human study perspective. For comparison, it is worth noting one study from the literature in which 39 human cytokines and chemokines (including IL-6, IL-8, and IL-10) were simultaneously quantified in pre- and post-dose plasma samples from 24 patients with cancer who were undergoing anti–PD-1 therapy. Only significant increases in IL-1 alpha and CXCL10 plasma levels were reported with anti–PD-1 alone post- versus pre-dose (48).

There are some limitations to the study that should be discussed. As already alluded to, the small sample size results in a lack of power to detect issues associated with a pro-inflammatory response. Larger studies may reveal other AEs not reported here; however, the broad similarity between the AEs reported here and those in the larger study in patients with cancer (35) is encouraging. There is also the question of the definition of immunosuppression used in the study; for practical purposes, an ALC of less than or equal to 1,100 cells/μL within the 96 hours before study treatment administration was employed. Reduced ALC correlates with worse outcomes in patients with sepsis (8, 10, 11). However, it is not a definitive marker of sepsis-associated immunosuppression. It is important to note that, in the current study, pre-dose mHLA-DR levels were very low (< 5,000 mAb/cell) (Fig. 2B), which would indicate that these participants were immunosuppressed and at increased risk of mortality (42, 49). Recently, an integrated genomics study of patients with sepsis admitted to the ICU also identified two distinct sepsis phenotypes: Sepsis Response Signature (SRS) 1 and SRS2, with SRS1 being an immunosuppressed phenotype (50). This immunosuppressed phenotype had lower ALC levels than the SRS2 phenotype (J. C. Knight, personal communication, 2017). Together, these findings provide a strong indication that lower ALC levels are indeed associated with immunosuppression in patients with sepsis.

These data suggest that PD-1/PD-L1 pathway inhibition in patients with sepsis-associated immunosuppression is well tolerated. BMS-936559, particularly with the higher doses studied, results in an apparent increase in mHLA-DR expression; further studies are needed to determine whether it safely restores immune functional status and protects against secondary infections and related clinical consequences (readmissions, morbidity, and mortality).

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This was the first clinical evaluation of an anti–PD-L1 (BMS-936559) in patients with sepsis-associated immunosuppression.

PD-1/PD-L1 pathway inhibition represents an evolution in conceptual understanding of approaches to sepsis treatment. Rather than seeking to inhibit the host response, checkpoint inhibitor therapies that enhance host immunity may represent a new way forward against this highly lethal syndrome. Further study of PD-1/PD-L1 pathway inhibition is warranted.

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Medical writing support was provided by Geraint Owens, PhD, of Chameleon Communications International, with funding from Bristol-Myers Squibb.

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1. Angus DC, van der Poll T. Severe sepsis and septic shock. N Engl J Med 2013; 369:840–851
2. Singer M, Deutschman CS, Seymour CW, et al. The third international consensus definitions for sepsis and septic shock (sepsis-3). JAMA 2016; 315:801–810
3. Fleischmann C, Scherag A, Adhikari NK, et al; International Forum of Acute Care Trialists: Assessment of global incidence and mortality of hospital-treated sepsis. Current estimates and limitations. Am J Respir Crit Care Med 2016; 193:259–272
4. Rhee C, Dantes R, Epstein L, et al; CDC Prevention Epicenter Program: Incidence and trends of sepsis in US hospitals using clinical vs claims data, 2009-2014. JAMA 2017; 318:1241–1249
5. Abraham E. Why immunomodulatory therapies have not worked in sepsis. Intensive Care Med 1999; 25:556–566
6. Marshall JC. Why have clinical trials in sepsis failed? Trends Mol Med 2014; 20:195–203
7. Hotchkiss RS, Tinsley KW, Swanson PE, et al. Sepsis-induced apoptosis causes progressive profound depletion of B and CD4+ T lymphocytes in humans. J Immunol 2001; 166:6952–6963
8. Inoue S, Suzuki-Utsunomiya K, Okada Y, et al. Reduction of immunocompetent T cells followed by prolonged lymphopenia in severe sepsis in the elderly. Crit Care Med 2013; 41:810–819
9. Boomer JS, To K, Chang KC, et al. Immunosuppression in patients who die of sepsis and multiple organ failure. JAMA 2011; 306:2594–2605
10. Stortz JA, Murphy TJ, Raymond SL, et al. Evidence for persistent immune suppression in patients who develop chronic critical illness after sepsis. Shock 2018; 49:249–258
11. Drewry AM, Samra N, Skrupky LP, et al. Persistent lymphopenia after diagnosis of sepsis predicts mortality. Shock 2014; 42:383–391
12. Keir ME, Francisco LM, Sharpe AH. PD-1 and its ligands in T-cell immunity. Curr Opin Immunol 2007; 19:309–314
13. Dong H, Strome SE, Salomao DR, et al. Tumor-associated B7-H1 promotes T-cell apoptosis: A potential mechanism of immune evasion. Nat Med 2002; 8:793–800
14. Bristol-Myers Squibb: Nivolumab (Opdivo) Prescribing Information. 2018. Available at: Accessed May 25, 2018
15. Genentech: Atezolizumab (Tecentriq) Prescribing Information. 2018. Available at: Accessed May 25, 2018
16. EMD Serono: Avelumab (Bavencio) Prescribing Information. 2017. Available at: Accessed May 25, 2018
17. AstraZeneca: Durvalumab (Imfinzi) Prescribing Information. 2018. Available at: Accessed May 25, 2018
18. Merck Sharp & Dohme: Pembrolizumab (Keytruda) Prescribing Information. 2017. Available at: Accessed May 25, 2018
19. Hotchkiss RS, Opal S. Immunotherapy for sepsis–a new approach against an ancient foe. N Engl J Med 2010; 363:87–89
20. Mansur A, Hinz J, Hillebrecht B, et al. Ninety-day survival rate of patients with sepsis relates to programmed cell death 1 genetic polymorphism rs11568821. J Investig Med 2014; 62:638–643
21. Venet F, Monneret G. Advances in the understanding and treatment of sepsis-induced immunosuppression. Nat Rev Nephrol 2018; 14:121–137
22. Sharpe AH, Wherry EJ, Ahmed R, et al. The function of programmed cell death 1 and its ligands in regulating autoimmunity and infection. Nat Immunol 2007; 8:239–245
23. Brown KE, Freeman GJ, Wherry EJ, et al. Role of PD-1 in regulating acute infections. Curr Opin Immunol 2010; 22:397–401
24. Brahmamdam P, Inoue S, Unsinger J, et al. Delayed administration of anti-PD-1 antibody reverses immune dysfunction and improves survival during sepsis. J Leukoc Biol 2010; 88:233–240
25. Zhang Y, Zhou Y, Lou J, et al. PD-L1 blockade improves survival in experimental sepsis by inhibiting lymphocyte apoptosis and reversing monocyte dysfunction. Crit Care 2010; 14:R220
26. Chang KC, Burnham CA, Compton SM, et al. Blockade of the negative co-stimulatory molecules PD-1 and CTLA-4 improves survival in primary and secondary fungal sepsis. Crit Care 2013; 17:R85
27. Wang JF, Li JB, Zhao YJ, et al. Up-regulation of programmed cell death 1 ligand 1 on neutrophils may be involved in sepsis-induced immunosuppression: An animal study and a prospective case-control study. Anesthesiology 2015; 122:852–863
28. Huang X, Venet F, Wang YL, et al. PD-1 expression by macrophages plays a pathologic role in altering microbial clearance and the innate inflammatory response to sepsis. Proc Natl Acad Sci U S A 2009; 106:6303–6308
29. Bankey PE, Banerjee S, Zucchiatti A, et al. Cytokine induced expression of programmed death ligands in human neutrophils. Immunol Lett 2010; 129:100–107
30. Guignant C, Lepape A, Huang X, et al. Programmed death-1 levels correlate with increased mortality, nosocomial infection and immune dysfunctions in septic shock patients. Crit Care 2011; 15:R99
31. Zhang Y, Li J, Lou J, et al. Upregulation of programmed death-1 on T cells and programmed death ligand-1 on monocytes in septic shock patients. Crit Care 2011; 15:R70
32. de Kleijn S, Langereis JD, Leentjens J, et al. IFN-γ-stimulated neutrophils suppress lymphocyte proliferation through expression of PD-L1. PLoS One 2013; 8:e72249
33. Chang K, Svabek C, Vazquez-Guillamet C, et al. Targeting the programmed cell death 1: Programmed cell death ligand 1 pathway reverses T cell exhaustion in patients with sepsis. Crit Care 2014; 18:R3
34. Patera AC, Drewry AM, Chang K, et al. Frontline science: Defects in immune function in patients with sepsis are associated with PD-1 or PD-L1 expression and can be restored by antibodies targeting PD-1 or PD-L1. J Leukoc Biol 2016; 100:1239–1254
35. Brahmer JR, Tykodi SS, Chow LQ, et al. Safety and activity of anti-PD-L1 antibody in patients with advanced cancer. N Engl J Med 2012; 366:2455–2465
36. Gay CL, Bosch RJ, Ritz J, et al; AIDS Clinical Trials 5326 Study Team: Clinical trial of the anti-PD-L1 antibody BMS-936559 in HIV-1 infected participants on suppressive antiretroviral therapy. J Infect Dis 2017; 215:1725–1733
37. Rivers EP, Jaehne AK, Nguyen HB, et al. Early biomarker activity in severe sepsis and septic shock and a contemporary review of immunotherapy trials: Not a time to give up, but to give it earlier. Shock 2013; 39:127–137
38. Dellinger RP, Levy MM, Rhodes A, et al; Surviving Sepsis Campaign Guidelines Committee including The Pediatric Subgroup: Surviving Sepsis Campaign: International guidelines for management of severe sepsis and septic shock, 2012. Intensive Care Med 2013; 39:165–228
39. Bristol-Myers Squibb: Sample analysis report for the quantification of BMS-936559 in human serum by Enzyme Linked Immunosorbent Assay [ELISA] in support of AI471049. Version No. 1.0. 2018, Document Control Number 930123383
40. Smith BP, Vandenhende FR, DeSante KA, et al. Confidence interval criteria for assessment of dose proportionality. Pharm Res 2000; 17:1278–1283
41. Bennett F, Luxenberg D, Ling V, et al. Program death-1 engagement upon TCR activation has distinct effects on costimulation and cytokine-driven proliferation: Attenuation of ICOS, IL-4, and IL-21, but not CD28, IL-7, and IL-15 responses. J Immunol 2003; 170:711–718
42. Pfortmueller CA, Meisel C, Fux M, et al. Assessment of immune organ dysfunction in critical illness: Utility of innate immune response markers. Intensive Care Med Exp 2017; 5:49
43. Döcke WD, Randow F, Syrbe U, et al. Monocyte deactivation in septic patients: Restoration by IFN-gamma treatment. Nat Med 1997; 3:678–681
44. Nakos G, Malamou-Mitsi VD, Lachana A, et al. Immunoparalysis in patients with severe trauma and the effect of inhaled interferon-gamma. Crit Care Med 2002; 30:1488–1494
45. Meisel C, Schefold JC, Pschowski R, et al. Granulocyte-macrophage colony-stimulating factor to reverse sepsis-associated immunosuppression: A double-blind, randomized, placebo-controlled multicenter trial. Am J Respir Crit Care Med 2009; 180:640–648
46. Francois B, Jeannet R, Daix T, et al. Interleukin-7 restores lymphocytes in septic shock: The IRIS-7 randomized clinical trial. JCI Insight 2018; 3:98960
47. Hakim FT, Memon SA, Cepeda R, et al. Age-dependent incidence, time course, and consequences of thymic renewal in adults. J Clin Invest 2005; 115:930–939
48. Das R, Verma R, Sznol M, et al. Combination therapy with anti-CTLA-4 and anti-PD-1 leads to distinct immunologic changes in vivo. J Immunol 2015; 194:950–959
49. Monneret G, Lepape A, Voirin N, et al. Persisting low monocyte human leukocyte antigen-DR expression predicts mortality in septic shock. Intensive Care Med 2006; 32:1175–1183
50. Davenport EE, Burnham KL, Radhakrishnan J, et al. Genomic landscape of the individual host response and outcomes in sepsis: A prospective cohort study. Lancet Respir Med 2016; 4:259–271

    antiprogrammed cell death-ligand 1 BMS-936559; immune checkpoint inhibition; immunotherapy; sepsis; sepsis-associated immunosuppression

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