Procalcitonin (ProCT), the precursor to the calcitonin hormone, is abnormally increased in experimental and clinical systemic inflammation, including sepsis. Initially, we investigated the effects of supraphysiologic amounts of ProCT administered to animals with septic peritonitis. Subsequently, we evaluated the efficacy of prophylactic and therapeutic immune blockade of ProCT in this lethal model of sepsis.
Prospective, experimental, controlled study.
Animal research laboratory approved by the American Association for the Accreditation of Laboratory Animal Care at a Veterans Affairs Medical Center.
Young male Golden Syrian hamsters, weighing 90 to 120 g.
In the first study, serum ProCT concentrations were measured in animals at 0, 3, 6, 12, and 24 hrs after induction of sepsis by intraperitoneal implantation of pellets containing Escherichia coli (5 x 108 colony-forming units/pellet). In the second study, with mortality as the end point, 30 [micro sign]g/kg of isolated, purified human ProCT in 10% hamster serum (experimental) or an equal volume of 10% hamster serum (control) were administered intravenously at the time of the E. coli peritoneal implantation. In the third study, experimental animals received intraperitoneal injections of a multiregion-specific goat antiserum reactive to hamster ProCT 1 hr before and 24 hrs after E. coli implantation, while control animals received nonimmune goat serum at the same time points. In the final study, the same antiserum was administered in five divided doses during the 24 hrs after the insertion of E. coli.
In the initial study, ProCT concentrations were increased shortly after induction of sepsis and peaked at 12 hrs. Administration of exogenous ProCT to septic animals significantly increased mortality compared with control animals (93% vs. 43%, p = .02). Prophylactic blockade of ProCT almost completely protected the animals from the lethal effects of sepsis: the 102-hr mortality rate in the experimental group was 6% compared with 62% in the control group (p < .003). In the therapeutic trial, the 102-hr mortality rate was 54% in experimental animals compared with 82% in control animals (p < .045).
These results demonstrate that increased ProCT exacerbates mortality in experimental sepsis, whereas neutralization of ProCT increases survival. Thus, ProCT, in addition to being an important marker of severity of systemic inflammation and mortality, is an integral part of the inflammatory process and directly affects the outcome. (Crit Care Med 1998; 26:1001-1006)
From the Section of Endocrinology, Department of Medicine (Drs. Nylen, Snider, and Becker), VA Medical Center, Washington, DC; Section of Endocrinology, Department of Medicine (Drs. Nylen and Becker), George Washington University Medical Center, Washington, DC; Department of Surgery (Dr. White), VA Medical Center, Washington, DC; Department of Surgery (Drs. Whang and White), Georgetown University, Washington, DC; Department of Surgery (Drs. Steinwald and White), George Washington University Medical Center, Washington, DC.
Supported, in part, by funds from the Department of Veterans Affairs.
Address requests for reprints to: Eric S. Nylen, MD, VAMC, 50 Irving Street, NW, Room GE 246, Washington, DC 20422.