Original ArticleGinsenoside Rb1 Alleviates Oxidative Low-Density Lipoprotein–Induced Vascular Endothelium Senescence via the SIRT1/Beclin-1/Autophagy AxisShi, Guangyao*; Liu, Dinghui*; Zhou, Bin*; Liu, Yong*; Hao, Baoshun*; Yu, Shujie*; Wu, Lin*; Wang, Min*; Song, Zhiming†; Wu, Chaodong‡; Zhu, Jieming*; Qian, Xiaoxian PhD*,§Author Information *Department of Cardiology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China; †Department of Cardiology, The First Affiliated Hospital of Henan University, Kaifeng, Henan, China; ‡Department of Nutrition and Food Science, Texas A&M University, College Station, TX; and §Institute Integrated Traditional Chinese and Western Medicine, Sun Yat-sen University, Guangzhou, Guangdong, China. Reprints: Jieming Zhu or Xiaoxian Qian, PhD, Department of Cardiology, The Third Affiliated Hospital of Sun Yat-sen University, 600 Tianhe Road, Guangzhou, 510630, China (e-mail: firstname.lastname@example.org or email@example.com). Supported by the grants from the National Natural Science Foundation of China (Grant Number: 81370447); Science and Technology Planning Project of Guangdong Province (grant number: 2016A050502014). The authors report no conflicts of interest. Supplemental digital content is available for this article. Direct URL citations appear in the printed text and are provided in the HTML and PDF versions of this article on the journal's Web site (www.jcvp.org). G. Shi, D. Liu, and B. Zhou have contributed equally. Received January 09, 2019 Accepted October 07, 2019 Journal of Cardiovascular Pharmacology: February 2020 - Volume 75 - Issue 2 - p 155-167 doi: 10.1097/FJC.0000000000000775 Buy SDC Metrics Abstract Oxidative low-density lipoprotein (ox-LDL) induces endothelium senescence and promotes atherosclerosis. Ginsenoside Rb1 (gRb1) has been proved to protect human umbilical vein cells (HUVECs), but its effect on ox-LDL–induced endothelium senescence and the underlying mechanism remains unknown. This study is to explore the involvement of the SIRT1/Beclin-1/autophagy axis in the effect of gRb1 on protecting endothelium against ox-LDL–induced senescence. Hyperlipidemia of Sprague Dawley rats was induced by high-fat diet, and gRb1 was intraperitoneal injected. A senescence model of HUVECs induced by ox-LDL was also established. The results showed that gRb1 alleviated hyperlipidemia-induced endothelium senescence and ox-LDL-induced HUVECs senescence. GRb1 also restored the reductions in SIRT1 and autophagy, which were involved in the anti-senescence effects. Beclin-1 acetylation was reduced, and the correlation between SIRT1 and Beclin-1 was increased by gRb1. Results of our study demonstrated the anti-senescence function of gRb1 against hyperlipidemia in the endothelium, and the underlying mechanism involves the SIRT1/Beclin-1/autophagy axis. Copyright © 2020 Wolters Kluwer Health, Inc. All rights reserved.