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Erythropoietin Improves Cardiovascular Function in Adult Rats After Acute Hemorrhage

Puchulu, María B., PhD*; Arreche, Noelia, PhD*; Zotta, Elsa, PhD*,†; Donato, Martin, PhD; Ogonowski, Natalia, PhD*; Fellet, Andrea, PhD*; Balaszczuk, Ana M., PhD*

Journal of Cardiovascular Pharmacology: May 2019 - Volume 73 - Issue 5 - p 290–300
doi: 10.1097/FJC.0000000000000666
Original Article

Abstract: Erythropoietin (EPO) has been linked to cardioprotective effects. However, its effects during the aging process are little known. We investigated the effect of EPO administration on hemodynamic parameters, cardiac function, oxidative damage, and erythropoietin receptor (EPOR) expression pattern in the hypovolemic state. EPO was administered (1000 IU/kg/3 days) and then acute hemorrhage (20% blood loss) was induced in young and adult rats. There was no difference in plasmatic EPO in either age group. The hemodynamic basal condition was similar, without alterations in renal function and hematocrit, in both age groups. After bleeding, both EPO-treated age groups had increased blood pressure at the end of the experimental protocol, being greater in adult animals. EPO attenuated the tachycardic effect. Ejection fraction and fractional shortening were higher in adult EPO-treated rats subjected to hemorrhage. In the left ventricle, young and adult EPO-treated rats subjected to bleeding showed an increased EPOR expression. A different EPOR expression pattern was observed in the adult right atrial tissue, compared with young animals. EPO treatment decreased oxidative damage to lipids in both age groups. EPO treatment before acute hemorrhage improves cardiovascular function during the aging process, which is mediated by different EPOR pattern expression in the heart tissue.

*Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Departamento de Ciencias Biológicas, Cátedra de Fisiología, Instituto de Química y Metabolismo del Fármaco, CONICET, Ciudad Autónoma de Buenos Aires, Argentina;

Universidad de Buenos Aires, Facultad de Medicina, Departamento de Fisiología, Instituto de Fisiología y Biofísica, CONICET, Ciudad Autónoma de Buenos Aires, Argentina; and

Universidad de Buenos Aires, Facultad de Medicina, Departamento de Patología, Instituto de Fisiolopatología Cardiovascular, CONICET, Ciudad Autónoma de Buenos Aires, Argentina.

Reprints: María B. Puchulu, PhD, Department of Physiology, School of Pharmacy and Biochemistry, University of Buenos Aires, IQUIMEFA-CONICET, Junín 956 7° floor, Buenos Aires 1113, Argentina (e-mail:

Supported by the University of Buenos Aires (UBACYT 20020130100045BA) and IQUIMEFA-National Scientific and Technical Research Council (CONICET).

The authors report no conflicts of interest.

Received October 16, 2018

Accepted February 05, 2019

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