Original ArticleSimvastatin Modulates Interaction Between Vascular Smooth Muscle Cell/Macrophage and TNF-α–Activated Endothelial CellChu, Fujiang MD*; Wang, Min MA*; Ma, Hongyan MD†; Zhu, Jiayong MA*Author Information *School of Basic Courses, Guangdong Pharmaceutical University, Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances, Guangzhou Higher Education Mega Center, Guangzhou, China; and †School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, China. Reprints: Jiayong Zhu, MA, School of Basic Courses, Guangdong Pharmaceutical University, Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances, Guangzhou Higher Education Mega Center, Guangzhou 510006, China (e-mail: firstname.lastname@example.org). Supported by National Natural Science Foundation of China (No. 81373960), Science and Technology Planning Project of Guangdong Province, China (No. 2014A020212416) and Guangzhou Science and Technology Plan Project, China (No. 201707010132). The authors report no conflicts of interest. Journal of Cardiovascular Pharmacology: May 2018 - Volume 71 - Issue 5 - p 268-274 doi: 10.1097/FJC.0000000000000567 Buy Metrics Abstract Cellular interactions between endothelial cell (EC) and vascular smooth muscle cell (VSMC)/macrophages seem to be greatly changed under inflammatory conditions. Although simvastatin could regulate inflammatory transcription factors in EC and VSMC and also could inhibit leukocyte–endothelium interaction, whether it could modulate VSMC/macrophage functions that are induced by tumor necrosis factor-α (TNF-α)–activated EC remained unclear. The purpose of this study was to investigate the effects of simvastatin on VSMC/macrophage functions, which are induced by TNF-α–activated EC in coculture system in vitro. The results showed that under noncontacting conditions, simvastatin could reduce the proliferation, apoptosis, and TNF-α, IL-6, and vascular endothelial growth factor secretion both in VSMC and macrophage, which is induced by TNF-α–activated EC. And a hypothesis that simvastatin regulates the interactions and the soluble factors between EC and VSMC/macrophages could be drawn. And that might be a potential anti-atherosclerosis mechanism of simvastatin. Copyright © 2018 Wolters Kluwer Health, Inc. All rights reserved.