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White Wine Consumption Influences Inflammatory Phase of Repair After Myocardial Infarction in Rats

Ključević, Nikola, MD*; Milat, Ana, Marija, MPhram; Grga, Mia, ChE; Mudnić, Ivana, PhD; Boban, Mladen, PhD; Grković, Ivica, PhD*

Journal of Cardiovascular Pharmacology: November 2017 - Volume 70 - Issue 5 - p 293–299
doi: 10.1097/FJC.0000000000000519
Original Article

Abstract: Effects of white wine (WW) consumption on the expression of inflammatory markers/mediators (MMP-2, MMP-9, NF-ĸB p65 and TGF-β1) in myocardial tissue after experimentally induced permanent myocardial ischemia was investigated. Male Sprague-Dawley rats were given either a combination of WW and water or only water, for 28 days. After coronary ligation, animals were left to survive for 24 hours. Three representative areas: infarct/ischemic, peri-infarct/border zone, and control/non-ischemic zones were analyzed for expression of immunoreactivity by measuring the threshold area % of signal density. For MMP-9, significantly smaller expression was found in all 3 zones of wine drinking animals (P < 0.001). There was no difference in MMP-2 immunoreactivity between the 2 groups, except in peri-infarct zones, where the signal was significantly decreased (P < 0.001). The same pattern of expression was found for the NF-κB p65 signal, although no differences between experimental groups were observed for TGF-β1. White wine consumption decreases the expression of the 3 investigated inflammatory markers/mediators in the peri-infarct zone, suggesting its significant modulatory effect. For MMP-9 and MMP-2, expression was similar to the effect of postischemic reperfusion. No effect on TGF-β1 was observed, highlighting its role in being the master-switch, changing from the inflammatory to the proliferative stage of infarct healing.

Departments of *Anatomy, Histology and Embryology; and

Pharmacology, University of Split School of Medicine, Split, Croatia.

Reprints: Ivica Grković, PhD, Department of Anatomy, Histology and Embryology, School of Medicine in Split, Šoltanska 2, 21000 Split, Croatia (e-mail:

This study was funded by the Croatian Science Foundation (Project no. 8652), whose support is gratefully acknowledged.

The authors report no conflicts of interest.

Received April 24, 2017

Accepted July 05, 2017

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