Original ArticlePuerarin Accelerates Re-Endothelialization in a Carotid Arterial Injury Model: Impact on Vasodilator Concentration and Vascular Cell FunctionsCheng, Min MD, PhD*; Li, Xin MD*; Guo, Zhiliang MD, PhD†; Cui, Xiaodong MD*; Li, Hong MD*; Jin, Chengwen MD*; Zhang, Xiaoyun MD*; Guan, Xiumei MD*Author Information *Medicine Research Center, Weifang Medical University, Weifang, Shandong, China; and †Department of Orthopedic, the 89th Hospital of Chinese PLA, Weifang, Shandong, China. Reprints: Min Cheng, MD, PhD, Medicine Research Center, Weifang Medical University, Weifang, Shandong 261053, China (e-mail: email@example.com). M. Cheng, X. Li, and Z.L. Guo contributed equally to this study. Supported by the National Natural Science Foundation of China (NO. 30900290, 31270993), the Shandong Provincial Natural Science Foundation (NO. ZR2009CQ027), the Program for New Century Excellent Talents in University (NO. NCET-10-0922), the Shandong Province Higher Educational Science and Technology Program (NO. J09LF06), and the Project sponsored by Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry. The authors have no conflicts of interest to disclose. Received April 29, 2013 Accepted May 28, 2013 Journal of Cardiovascular Pharmacology: October 2013 - Volume 62 - Issue 4 - p 361-368 doi: 10.1097/FJC.0b013e31829dd961 Buy Metrics Abstract Abstract: Puerarin, a main isoflavone glucoside derived from the Chinese medicine Radix puerariae, has been employed clinically to prevent and treat various cardiovascular disorders. However, little research has been performed to identify the in vivo effects of puerarin on the re-endothelialization and neointimal hyperplasia of injured vessels, and its detailed mechanisms of action remain to be elucidated. In this study, Sprague–Dawley rats were treated with puerarin at the dosages of 0, 50, and 100 mg·kg−1·day−1 i.p. after balloon carotid denudation for 2 weeks. The results showed that puerarin accelerated re-endothelialization after surgery, resulting in a significant reduction of neointima formation. Moreover, puerarin increased the serum levels of vasodilators, such as nitric oxide and prostaglandin I2, in a dose-dependent manner. In vitro, puerarin exhibited protective effects on late endothelial progenitor cells and mature endothelial cells, and inhibitory effects on the migration of vascular smooth muscle cells. Taken together, these data indicate that puerarin accelerates re-endothelialization, inhibits neointima formation, and attenuates vascular remodeling at sites of arterial injury, possibly due to the cytoprotective effects on endothelial lineage and the suppression of vascular smooth muscle cell migration. © 2013 by Lippincott Williams & Wilkins.