Original ArticleA Simple Modification Results in Greater Success in the Model of Coronary Artery Ligation and Myocardial Ischemia in MiceLiao, Xiao-Long MD*,†,‡; Hu, Xiao-Xia BS*,†,‡; Chang, Feng-Jun BS*,†,‡; Yuan, Hai-Yun MS*,†,‡; Ci, Hong-Bo MS*,†,‡; Wu, Jia-Yun BS*,†,‡; Xu, Zhe MS*,†,‡; Wang, Zhi-Ping MD, PhD*,†,‡; Zhang, Xi MD*,†,‡; Xia, Zhengyuan MD, PhD§; Ou, Jing-Song MD, PhD, FCCP*,†,‡Author Information *Division of Cardiac Surgery; †The Key Laboratory of Assisted Circulation, Ministry of Health; ‡Guangdong Engineering Laboratory for Diagnosis and Treatment of Vascular Diseases, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China; and §Department of Anesthesiology, The University of Hong Kong, Hong Kong SAR, China. Reprints: Jing-Song Ou, MD, PhD, FCCP, Division of Cardiac Surgery, The First Affiliated Hospital, Sun Yat-sen University, 58 Zhong Shan Er Road, Guangzhou 510080, China (e-mail: firstname.lastname@example.org). Supported by grants from the National Natural Science Foundation of China (30971261, 81170271), Guangdong Pearl River Scholars Program, the Ministry of Education of China (20100171110057), the Science and Technology Research for the Returned Overseas Chinese Scholars from the Ministry of Human Resources and Social Security of China, Guangdong Natural Science Fund Committee, China (9251008901000003), and the Bureau of Science and Information Technology of Guangzhou, China (2010GN-E00221). The authors report no conflicts of interest. Received December 02, 2012 Accepted January 18, 2013 Journal of Cardiovascular Pharmacology: May 2013 - Volume 61 - Issue 5 - p 430-436 doi: 10.1097/FJC.0b013e318288a68d Buy Metrics Abstract Abstract: Mouse models of myocardial ischemic preconditioning (IPC) and ischemic postconditioning (IPD) have proven to be very useful models of cardiovascular diseases. In 2010, Gao described a novel procedure without the aid of mechanical ventilation. However, the technique of heart externalization could not be applied to mouse models of IPC or IPD due to the limited time frame of the technique. We proposed a modified simple and safe method using lung recruitment and short-term ventilation to perform the procedure in mice with IPC or IPD. The mice were randomly divided into 4 groups: the modified groups, M-IPC and M-IPD, and the conventional groups, C-IPC and C-IPD. In the 2 modified groups, the mice were removed from the ventilator and allowed to resume breathing spontaneously upon completion of the lung recruitment and the rapid closure of the thorax. Our study demonstrated that the postoperative recovery time was significantly reduced for the modified groups compared with the 2 conventional groups. Moreover, the inflammatory damages were attenuated by the modified method compared with the conventional method. In addition, the modified method significantly increased the survival rates of mice with IPC or IPD. The modified method improved the survival rates of mouse models of myocardial ischemia. © 2013 Lippincott Williams & Wilkins, Inc.