ENDOTHELINS AND ENDOTHELIN-CONVERTING ENZYMES: EXPRESSION, MODULATION AND ACTIVITY: PDF OnlySaida Kaname; Uchide, Tsuyoshi; Usui, Akemi; Gao, Xiao-dong; Tomizuka, Noboru; Oka, Shu-ichi; Masuda, HiromiJournal of Cardiovascular Pharmacology: 2000 - p S9-S11 Free Abstract Summary: In order to understand the physiological roles of vasoactive intestinal contractor (VIC)/endothelin-2 (ET-2), we examined the expression of this peptide by specific reverse transcriptase polymerase chain reaction (RT-PCR) analysis and found that PC12 rat pheochromocytoma cells express the VIC gene. The 5'-flanking 1.0 kilo base pair (kb) region of the mouse VIC gene is sufficient to express a secreted alkaline phosphatase (SEAP) reporter gene in transiently transfected PC12 cells. The 1.0 kb promoter region may contain cis-acting elements that determine the rate of the VIC gene transcription in PC12 cells. Address correspondence and reprint requests to Kaname Saida, Biosignaling Department, National Institute of Bioscience and Human Technology, Agency of Industrial Science and Technology, 1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan. © 2000 Lippincott Williams & Wilkins, Inc.