The distribution of endothelin-1 (ET-1) and its receptors (ETA/ETB) has been studied in segments of femoral artery obtained from patients undergoing operation for peripheral vascular disease (PVD) using a combination of immunohistochemistry and autoradiography. Both receptor subtypes were located on the tunica media of vessel segments, with ETA-receptors predominating. Densitometric analysis showed that there was no difference in receptor binding to proximal/distal arterial segments from PVD patients. High-resolution autoradiography identified ETB binding to vascular nerves and vasa vasorum, mainly in distal portions of femoral arteries. Immunoreactive ET-1 was also identified that was associated with the vasa vasorum.
Departments of *Molecular Pathology & Clinical Biochemistry and †Surgery, Royal Free & University College Medical School, Pond Street, London, and ‡Division of Cardiology, Imperial College School of Medicine, Hammersmith Campus, Du Cane Road, London, U.K.
Address correspondence and reprint requests to Michael R Dashwood, Department of Molecular Pathology & Clinical Biochemistry, Royal Free & University College Medical School, Royal Free Campus, Pond Street, London NW3 2QG, U.K.
© 2000 Lippincott Williams & Wilkins, Inc.