Original Article: PDF OnlyRenin-Angiotensin System in Cultured Human Arterial Smooth Muscle CellsLarrue, J.; Demond-Henri, J.; Daret, D.Author Information INSERM U8. Pessac, France Journal of Cardiovascular Pharmacology: Volume 14 - Issue - p S43-S45 Free Abstract Angiotensin converting enzyme (ACE) inhibitors were evaluated from their effects on human arterial smooth muscle cells in culture. [3H]ramipril binding has been determined in two different cell lines exhibiting ACE( + ) and ACE(-) phenotypes. Specific binding occurred only in ACE(+) cells, was saturable, and displayed a Kd of 1 nM with a β max value of 3.5 fmol/106 cells. Ramipril specific binding did not significantly modulate PGI2 synthesis in ACE( + ) cells. By contrast, ramipril and, to a lesser extent, captopril appeared as weak inhibitors of PGI2 synthesis in ACE(-) cells. These data indicate that human arterial SMCs may express several phenotypes of the reninangiotensin system under culture conditions and that ACE inhibitors may exert a non-renin-angiotensinmediated pharmacological effect on eicosanoid synthesis. Copyright © 1989 Wolters Kluwer Health, Inc. All rights reserved.